Design of reinforced concrete frame structure of exhibition hall

Import 23/08/2017Předmětem bakalářské práce je železobetonová dvoupatrová rámová konstrukce, která má sloužit pro občanskou vybavenost jako výstavní síň. Cílem je navrhnout a posoudit nosné prvky rámové konstrukce, konstrukce schodiště a stropů a založení na základových patkách podle metody mezních stavů, platných norem a konstrukčních zásad. Pro posouzené prvky byly vypracovány výkresy výztuže a stavební výkresy.The subject of Bachelor thesis is two storey reinforced concrete frame structure, which has to serve for civil amenitied as exhibition hall. The aim is to design and assess the support elements of frame structure, the structure of staircase and the ceilings and the foundation on foundation plinths according to the method of limit states, applicable standards and design principles. For assess elements have been created reinforcement drawings and constuction drawings.221 - Katedra konstrukcívýborn

Growth curves of <i>S</i>. <i>aureus</i> at sub-inhibitory concentrations of baicalein.

<p>Baicalein at 16, 32 and 64 μg/mL had no effect on bacterial growth.</p

Relative expression of the <i>ica</i>(using 16S rRNA / <i>gyrA</i> / <i>gmk</i> as the internal parametric gene).

<p>Comparative measurement of relative expression shown as an average of 3 experiments (★ compared to the control group, P < 0.05).</p

SEA detected by western blot.

<p>Upper: Western blot analysis of SEA from control group and groups treated with 64, 32, and 16 μg/ml baicalein for 5 h. Lower: Band intensities were quantitated by densitometry. Each lane was divided by the logarithmic number of cells in order to evaluate the amount of SEA per cell. (<b>*</b> P < 0.05, compared to the control group with one-way ANOVA). Data shown are the average of 3 experiments.</p

Impact of baicalein as assessed by fluorescence microscopy(200×).

<p>Static biofilms after exposure to baicalein for 3 days (top) and 7 days (bottom) were assessed by SYTO9. <i>S</i>. <i>aureus</i> within biofilms on polystyrene slices diplay green fluorescence. Controls group (A, E); 16 μg/ml baicalein group(B, F); 32 μg/ml baicalein group (C,G); 64 μg/ml baicalein group (D,H) A, E: control group; B, F: 16 μg/mL baicalein group; C, G: 32 μg/mL baicalein group; D, H: 64 μg/mL baicalein group.</p

Relative expression of the <i>hla</i> (using 16sRNA as the internal parametric gene).

<p>Comparative measurement of the relative expression shown as average of 3 experiments. (★ compared to the control group, P < 0.001).</p

Relative expression of the RNAIII (using 16S rRNA / <i>gyrA</i> / <i>gmk</i> as the internal parametric gene).

<p>Comparative measurement of relative expression shown as an average of 3 experiments (★ compared to the control group, P < 0.05).</p

Baicalein Inhibits <i>Staphylococcus aureus</i> Biofilm Formation and the Quorum Sensing System <i>In Vitro</i> - Fig 5

<p><b>(a) Use of baicalein as adjunct therapy with conventional antibiotics for 3-day biofilms.</b> Biofilms were formed on polystyrene carriers for 3 days by growing of <i>S</i>. <i>aureus</i> in TSB-G, then treating with VCM alone or combination with baicalein or CLR for 12 h (* compared to the control group, P < 0.001; # compared to the CLR+VCM group, P < 0.05; average of 3 experiments). <b>(b) Three-day biofilm scanning by SEM (×15000)</b> A: control group; B: 4 μg/mL VCM; C: 16 μg/mL CLR; D: 16 μg/mL CLR + 4 μg/mL VCM; E: 32 μg/mL baicalein; F: 32 μg/mL baicalein + 4 μg/mL VCM. <b>(c) Three-day biofilm scanning by CLSM (×400)</b> A: control group; B: 4 μg/mL VCM C: 32 μg/mL baicalein; D: 32 μg/mL baicalein + 4 μg/mL VCM.</p

Relative expression of the <i>agrA</i> (using 16S rRNA / <i>gyrA</i> / <i>gmk</i> as the internal parametric gene).

<p>Comparative measurement of the relative expression shown as average of 3 experiments. (★ compared to the control group, P < 0.05).</p

Relative expression of the <i>sarA</i> (using 16S rRNA / <i>gyrA</i> / <i>gmk</i> as the internal parametric gene).

<p>Comparative measurement of relative expression shown as an average of 3 experiments (★ compared to the control group, P < 0.05).</p