Daylight-activated fumigant detoxifying nanofibrous membrane based on thiol-ene click chemistry

Daylight-activated detoxifying nanofibrous membranes (LDNMs) are fabricated by grafting benzophenone-3,3',4,4'-tetracarboxylic dianhydride (BD) and biological thiols successively on poly(vinyl alcohol-co-ethylene) (EVOH) nanofibrous membrane. Taking the merits of photoactivity of BD, high-reactivity of biological thiols, and high specific surface area and porosity of the nanofibrous membrane, 1,3-dichloropropene (1,3-D) can be efficiently detoxified on the LDNMs under daylight irradiation via a thiol-ene click reaction. The detoxification function of the LDNMs is "switched on" by light irradiation and continues by following a cascade of chemical attacks of thiyl radicals formed during the photoexcitation process. The resultant LDNMs present rapid detoxification rate (i.e., t1/2 =~30 min) and massive detoxification amount (i.e., ~12 mg/g) against 1,3-D vapor under ambient conditions. More importantly, the LDNMs perform a detoxification tailing effect after moving the light-irradiated membrane to a dark environment, thus ensuring the protective function in the absence of sufficient light sources. The detoxification property of the LDNMs in an outdoor environment with sunlight irradiation shows comparable results to the lab-scale outcomes, enabling them to serve as innovative materials for personal protective equipment in practical applications. The successful fabrication of LDNMs may inspire new insights into the design of protective materials providing aggressive protection

Loss-of-function mutations with circadian rhythm regulator Per1/Per2 lead to premature ovarian insufficiency

The mechanism underlying premature ovarian insufficiency remains incompletely understood. Here we report that mice with Per1m/m; Per2m/m double mutations display a decrease in female fertility starting approximately at 20 weeks old, with significantly less pups born from 32 weeks old onwards. Histological analysis revealed that a significant reduction of ovarian follicles was observed in the Per1/Per2 mutants compared with the littermate controls examined at 26 and 52 weeks old, while the difference was not statistically significant between the two groups at 3 and 8 weeks old. We further showed that vascular development including the ovarian follicle associated vascular growth appeared normal in the Per1/Per2 mutant mice, although clock genes were reported to regulate angiogenesis in zebrafish. The findings imply that loss-of-function mutations with Per1/Per2 result in a premature depletion of ovarian follicle reserve leading to the decline of reproductive capacity.Peer reviewe

Correction : Control of PD-L1 expression by miR-140/142/340/383 and oncogenic activation of the OCT4-miR-18a pathway in cervical cancer.

This research was supported by a grant from the Department of Women’s Health Educational System, JSPS Grant-in-Aid for Scientific Research (C) (15K10697 and 16K11123) and the Science and Technology Planning Project of Guangdong Province, China (2014A020212124). We thank Dr. Zhujie Xu for experimental assistance. The authors declare that they have no conflict of interest.Peer reviewedPublisher PD

Identification of Colletotrichum horii Isolated from Postharvest Fig Anthracnose and Its Control by Bacillus velezensis

Anthracnose, a common postharvest fungal disease of fruits, can cause severe economic losses. A pathogenic strain named FC.006 was isolated from postharvest anthracnose of ‘Browns Wick’ figs by the traditional fungal isolation method. FC.006 was identified as Colletotrichum horii by morphological observation, multigene sequence identification and phylogenetic tree analysis. In order to investigate a biological control method for FC.006, the control effect of the biocontrol agent Bacillus velezensis RD.006 on FC.006 in vitro and infected figs was evaluated. The results showed that the inhibition rate of FC.006 by RD.006 was 85% when co-cultured in vitro for 8 days. RD.006 had good disease control effect on figs infected with FC.006, and significantly increased the expression of the FcPAL, Fc4CL, FcC4H, FcCAT, FcAPX, and FcPOD genes at the early stage after inoculation (P < 0.05). To sum up, C. horii FC.006 can cause fig anthracnose, and Bacillus velezensis RD.006 can effectively control postharvest anthracnose in figs through direct antibiosis and induction of fruit disease resistance

SMAD2/3 Signaling in the Uterine Epithelium Controls Endometrial Cell Homeostasis and Regeneration

The regenerative potential of the endometrium is attributed to endometrial stem cells; however, the signaling pathways controlling its regenerative potential remain obscure. In this study, genetic mouse models and endometrial organoids are used to demonstrate that SMAD2/3 signaling controls endometrial regeneration and differentiation. Mice with conditional deletion of SMAD2/3 in the uterine epithelium using Lactoferrin-iCre develop endometrial hyperplasia at 12-weeks and metastatic uterine tumors by 9-months of age. Mechanistic studies in endometrial organoids determine that genetic or pharmacological inhibition of SMAD2/3 signaling disrupts organoid morphology, increases the glandular and secretory cell markers, FOXA2 and MUC1, and alters the genome-wide distribution of SMAD4. Transcriptomic profiling of the organoids reveals elevated pathways involved in stem cell regeneration and differentiation such as the bone morphogenetic protein (BMP) and retinoic acid signaling (RA) pathways. Therefore, TGFβ family signaling via SMAD2/3 controls signaling networks which are integral for endometrial cell regeneration and differentiation

Lipopolysaccharide-induced depression-like model in mice: meta-analysis and systematic evaluation

Depression is a complex and biologically heterogeneous disorder. Recent studies have shown that central nervous system (CNS) inflammation plays a key role in the development of depression. Lipopolysaccharide (LPS)-induced depression-like model in mice is commonly used to studying the mechanisms of inflammation-associated depression and the therapeutic effects of drugs. Numerous LPS-induced depression-like models in mice exist and differ widely in animal characteristics and methodological parameters. Here, we systematically reviewed studies on PubMed from January 2017 to July 2022 and performed cardinal of 170 studies and meta-analyses of 61 studies to support finding suitable animal models for future experimental studies on inflammation-associated depression. Mouse strains, LPS administration, and behavioral outcomes of these models have been assessed. In the meta-analysis, forced swimming test (FST) was used to evaluate the effect size of different mouse strains and LPS doses. The results revealed large effect sizes in ICR and Swiss mice, but less heterogeneity in C57BL/6 mice. For LPS intraperitoneal dose, the difference did not affect behavioral outcomes in C57BL/6 mice. However, in ICR mice, the most significant effect on behavioral outcomes was observed after the injection of 0.5 mg/kg LPS. Our results suggests that mice strains and LPS administration play a key role in the evaluation of behavioral outcomes in such models

FXR Acts as a Metastasis Suppressor in Intrahepatic Cholangiocarcinoma by Inhibiting IL-6-Induced Epithelial-Mesenchymal Transition

Background/Aims: Intrahepatic cholangiocarcinoma (ICC) is a complicated condition, with difficult diagnosis and poor prognosis. The expression and clinical significance of the farnesoid X receptor (FXR), an endogenous receptor of bile acids, in ICC is not well understood. Methods: Western blotting and immunochemical analyses were used to determine the levels of FXR in 4 cholangiocarcinoma cell lines, a human intrahepatic biliary epithelial cell line (HIBEpic) and 322 ICC specimens, respectively, while quantitative reverse transcription polymerase chain reaction was used to detect the mRNA levels of FXR in cholangiocarcinoma cell lines. We evaluated the prognostic value of FXR expression and its association with clinical parameters. We determined the biological significance of FXR in ICC cell lines by agonist-mediated activation and lentivirus-mediated silence. IL-6 expression was tested by an enzyme-linked immunosorbent assay and flow cytometry. In vitro, cell proliferation was examined by Cell Counting Kit-8, migration and invasion were examined by wound healing and transwell assays; in vivo, tumor migration and invasion were explored in NOD-SCID mice. Results: FXR was downregulated in ICC cell lines and clinical ICC specimens. Loss of FXR was markedly correlated with aggressive tumor phenotypes and poor prognosis in patients with ICC. Moreover, FXR expression also had significant prognostic value in carbohydrate antigen 19-9 (CA19-9) negative patients. The expression of FXR was negatively correlated with IL-6 levels in clinical ICC tissues. FXR inhibited the proliferation, migration, invasion and epithelial mesenchymal transition (EMT) of ICC cells via suppression of IL-6 in vitro. Obeticholic acid, an agonist of FXR, inhibited IL-6 production, tumor growth and lung metastasis of ICC in vivo. Conclusions: FXR could be a promising ICC prognostic biomarker, especially in CA19-9 negative patients with ICC. FXR inhibits the tumor growth and metastasis of ICC via IL-6 suppression

What We Are Learning from COVID-19 for Respiratory Protection: Contemporary and Emerging Issues

Infectious respiratory diseases such as the current COVID-19 have caused public health crises and interfered with social activity. Given the complexity of these novel infectious diseases, their dynamic nature, along with rapid changes in social and occupational environments, technology, and means of interpersonal interaction, respiratory protective devices (RPDs) play a crucial role in controlling infection, particularly for viruses like SARS-CoV-2 that have a high transmission rate, strong viability, multiple infection routes and mechanisms, and emerging new variants that could reduce the efficacy of existing vaccines. Evidence of asymptomatic and pre-symptomatic transmissions further highlights the importance of a universal adoption of RPDs. RPDs have substantially improved over the past 100 years due to advances in technology, materials, and medical knowledge. However, several issues still need to be addressed such as engineering performance, comfort, testing standards, compliance monitoring, and regulations, especially considering the recent emergence of pathogens with novel transmission characteristics. In this review, we summarize existing knowledge and understanding on respiratory infectious diseases and their protection, discuss the emerging issues that influence the resulting protective and comfort performance of the RPDs, and provide insights in the identified knowledge gaps and future directions with diverse perspectives.This article is published as Li, Rui, Mengying Zhang, Yulin Wu, Peixin Tang, Gang Sun, Liwen Wang, Sumit Mandal et al. "What We Are Learning from COVID-19 for Respiratory Protection: Contemporary and Emerging Issues." Polymers 13, no. 23 (2021): 4165. doi: https://doi.org/10.3390/polym13234165. © 2021 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (https://creativecommons.org/licenses/by/4.0/)

Immunogenomic Intertumor Heterogeneity Across Primary and Metastatic Sites in a Patient With Lung Adenocarcinoma

Background: Lung cancer is the leading cause of cancer death, partially owing to its extensive heterogeneity. The analysis of intertumor heterogeneity has been limited by an inability to concurrently obtain tissue from synchronous metastases unaltered by multiple prior lines of therapy. Methods: In order to study the relationship between genomic, epigenomic and T cell repertoire heterogeneity in a rare autopsy case from a 32-year-old female never-smoker with left lung primary late-stage lung adenocarcinoma (LUAD), we did whole-exome sequencing (WES), DNA methylation and T cell receptor (TCR) sequencing to characterize the immunogenomic landscape of one primary and 19 synchronous metastatic tumors. Results: We observed heterogeneous mutation, methylation, and T cell patterns across distinct metastases. Only TP53 mutation was detected in all tumors suggesting an early event while other cancer gene mutations were later events which may have followed subclonal diversification. A set of prevalent T cell clonotypes were completely excluded from left-side thoracic tumors indicating distinct T cell repertoire profiles between left-side and non left-side thoracic tumors. Though a limited number of predicted neoantigens were shared, these were associated with homology of the T cell repertoire across metastases. Lastly, ratio of methylated neoantigen coding mutations was negatively associated with T-cell density, richness and clonality, suggesting neoantigen methylation may partially drive immunosuppression. Conclusions: Our study demonstrates heterogeneous genomic and T cell profiles across synchronous metastases and how restriction of unique T cell clonotypes within an individual may differentially shape the genomic and epigenomic landscapes of synchronous lung metastases

Pliensbachian (early Jurassic) deep-time peatland evolution in Northwest China driven by climate change

As peat deposits represent significant terrestrial carbon sinks, the processes of peatland initiation, evolution and termination are important components of the global carbon cycle. Research on recent peatlands leaves many questions unanswered concerning peatland evolution and the driving mechanisms for changes in peat forming environments in deep-time. Using a combination of sedimentology, coal petrology and palynology, this study investigates coal seams #B, #C and #D from the Pliensbachian (Early Jurassic) Qaidam Basin in China to elucidate the evolution of mire types and vegetation in their precursor peats, and to evaluate paleoenvironmental conditions during peat formation. Based on ash yield, total sulfur contents and coal maceral proxies, coals #B and #C record rheotrophic swamp forest mires that gradually transitioned to ombrotrophic bog forest mire conditions. Coal #D has a more complex evolution, initially forming under the ombrotrophic bog forest mire conditions that characterized coal #C before transitioning to a rheotrophic mire with initially fen and then wet swamp forest conditions. Finally, coal #D records a reversion to ombrotrophic mire conditions dominated by bog forest. Palynological assemblages and the ratios of hygrophytic (H) to xerophytic (X) plants reveal a remarkable change in peat-forming vegetation and paleoclimate. Coals #B and #C are dominated by woody gymnosperms, while deposition of the precursor peats of coal #D gradually evolved into a mix of woody and herbaceous plants. This floristic transition coincided with intensified climatic oscillations, establishing cyclic dry-wet conditions during the later stages of coal #D deposition. Our results reveal that during the Pliensbachian in the Qaidam Basin, climate forcing on peatland environments was manifested primarily through changes in precipitation and water availability brought on by intensification of seasonality. Peatland evolution was a multi-phase process of changing mire types and an overall vegetation succession from woody to herbaceous and woody plants