The Proteome of Biologically Active Membrane Vesicles from Piscirickettsia salmonis LF-89 Type Strain Identifies Plasmid-Encoded Putative Toxins

Indexación: Scopus.Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs) released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology. Initially, the cytotoxicity of different MV concentration purified from P. salmonis LF-89 was confirmed in an in vivo adult zebrafish infection model. The cumulative mortality of zebrafish injected with MVs showed a dose-dependent pattern. Analyses identified 452 proteins of different subcellular origins; most of them were associated with the cytoplasmic compartment and were mainly related to key functions for pathogen survival. Interestingly, previously unidentified putative virulence-related proteins were identified in P. salmonis MVs, such as outer membrane porin F and hemolysin. Additionally, five amino acid sequences corresponding to the Bordetella pertussis toxin subunit 1 and two amino acid sequences corresponding to the heat-labile enterotoxin alpha chain of Escherichia coli were located in the P. salmonis MV proteome. Curiously, these putative toxins were located in a plasmid region of P. salmonis LF-89. Based on the identified proteins, we propose that the protein composition of P. salmonis LF-89 MVs could reflect total protein characteristics of this P. salmonis type strain. © 2017 Oliver, Hernández, Tandberg, Valenzuela, Lagos, Haro, Sánchez, Ruiz, Sanhueza-Oyarzún, Cortés, Villar, Artigues, Winther-Larsen, Avendaño-Herrera and Yáñez.https://www.frontiersin.org/articles/10.3389/fcimb.2017.00420/ful

Present and Future Observing Trends in Atmospheric Magnetoseismology

With modern imaging and spectral instruments observing in the visible, EUV, X-ray, and radio wavelengths, the detection of oscillations in the solar outer atmosphere has become a routine event. These oscillations are considered to be the signatures of a wave phenomenon and are generally interpreted in terms of magnetohydrodynamic (MHD) waves. With multiwavelength observations from ground- and space-based instruments, it has been possible to detect waves in a number of different wavelengths simultaneously and, consequently, to study their propagation properties. Observed MHD waves propagating from the lower solar atmosphere into the higher regions of the magnetized corona have the potential to provide excellent insight into the physical processes at work at the coupling point between these different regions of the Sun. High-resolution wave observations combined with forward MHD modeling can give an unprecedented insight into the connectivity of the magnetized solar atmosphere, which further provides us with a realistic chance to reconstruct the structure of the magnetic field in the solar atmosphere. This type of solar exploration has been termed atmospheric magnetoseismology. In this review we will summarize some new trends in the observational study of waves and oscillations, discussing their origin and their propagation through the atmosphere. In particular, we will focus on waves and oscillations in open magnetic structures ( e.g., solar plumes) and closed magnetic structures ( e.g., loops and prominences), where there have been a number of observational highlights in the past few years. Furthermore, we will address observations of waves in filament fibrils allied with a better characterization of their propagating and damping properties, the detection of prominence oscillations in UV lines, and the renewed interest in large-amplitude, quickly attenuated, prominence oscillations, caused by flare or explosive phenomena