Modulation of micro-rheological and hematological parameters in the presence of artificial carotid-jugular fistula in rats

Search term Clear input Advanced Help Result Filters Format: Abstract Send to Clin Hemorheol Microcirc. 2018 Jun 9. doi: 10.3233/CH-180411. [Epub ahead of print] Modulation of micro-rheological and hematological parameters in the presence of artificial carotid-jugular fistula in rats. Ghanem S1, Somogyi V1, Tanczos B1, Szabo B1, Deak A1, Nemeth N1. Author information Abstract BACKGROUND: Arteriovenous fistula (AVF) may affect erythrocytes through many pathways (e.g., mechanical, inflammatory). However, these effects haven't been elucidated completely yet. OBJECTIVE: To follow-up the hemorheological and the hematological changes in the presence of artificial carotid-jugular fistula in rats. METHODS: Female Wistar rats were subjected to sham-operated group (SG, n = 6) and to fistula group (FG, n = 10). Under general anesthesia, the right carotid artery and jugular vein were isolated via a neck incision, and in the FG carotid-jugular fistula was performed by microsurgical techniques Hematological variables, red blood cell (RBC) deformability and membrane (mechanical) stability parameters were determined before operation and on the 1st and 6th postoperative weeks. Density separated samples ('young' and 'old' RBCs) were also tested. RESULTS: In FG group hematocrit, RBC and platelet counts increased gradually to reach highly significant level of increment on the 6th postoperative week. RBC deformability significantly was impaired. The membrane stability test showed lower deformability values after applying mechanical shearing. No significant differences were observed between density separated RBC subpopulations. CONCLUSIONS: The presence of arterio-venous fistula may lead to an increment of RBC mass and impairment of RBC deformability. These changes could be one of the pathways through which the fistula influences the microcirculation

Hemorheological factors can be informative in comparing treatment possibilities of abdominal compartment syndrome

BACKGROUND: Abdominal compartment syndrome (ACS) is a life-threatening condition, of which pathomechanism hasn’t been completely clarified, yet. Furthermore, surgical therapy still needs optimization. OBJECTIVE: We aimed to investigate microcirculatory and micro-rheological alterations in ACS, using various temporary abdominal closure methods, including three settings of vacuum-assisted closure technique (negative pressure wound therapy, NPWT). METHODS: On anesthetized pigs, by intraabdominally placed and filled-up silicone bags, intraabdominal pressure at 30 mmHg was maintained for 3 hours, and afterwards, decompressive laparotomy happened. In different experimental groups Bogota-bag or Vivano-sets were applied (–50, –100, –150 mmHg) for 2 hours. Pressure monitoring was done by implanted sensors, hemorheological parameters were determined, and laser Doppler flowmetry tests were performed on the surface of intraabdominal organs. RESULTS: Treatment with Bogota-bag and –150 mmHg vacuum increased erythrocyte aggregation, while deformability declined. Blood viscosity increased after treatment with –150 mmHg vacuum. The microcirculatory parameters of the NPWT groups were better in case of the small intestine. CONCLUSIONS: ACS resulted in impairment of macro- and micro-rheological parameters and abdominal organs’ microcirculation. All of the used techniques improved the results, however, applying Bogota-bag or –150 mmHg vacuum set showed worse microcirculatory and micro-rheological data than the settings at –100 or –50 mmHg