786 research outputs found
The isotropic-nematic interface in suspensions of hard rods: Mean-field properties and capillary waves
We present a study of the isotropic-nematic interface in a system of hard
spherocylinders. First we compare results from Monte Carlo simulations and
Onsager density functional theory for the interfacial profiles of the
orientational order parameter and the density. Those interfacial properties
that are not affected by capillary waves are in good agreement, despite the
fact that Onsager theory overestimates the coexistence densities. Then we show
results of a Monte Carlo study of the capillary waves of the interface. In
agreement with recent theoretical investigations (Eur.Phys.J. E {\bf 18} 407
(2005)) we find a strongly anistropic capillary wave spectrum. For the
wave-numbers accessed in our simulations, the spectrum is quadratic,
i.e.elasticity does not play a role. We conjecture that this effect is due to
the strong bending rigidity of the director field in suspensions of
spherocylinders.Comment: 8 pages, 10 figure
Targeted protein delivery: carbodiimide crosslinking influences protein release from microparticles incorporated within collagen scaffolds
open access articleTissue engineering response may be tailored via controlled, sustained release of active agents from protein-loaded degradable microparticles incorporated directly within three-dimensional (3D) ice-templated collagen scaffolds. However, the effects of covalent crosslinking during scaffold preparation on the availability and release of protein from the incorporated microparticles have not been explored. Here, we load 3D ice-templated collagen scaffolds with controlled additions of poly-(DL-lactide-co-glycolide) microparticles. We probe the effects of subsequent N-(3-dimethylaminopropyl)- N0-ethylcarbodiimide hydrochloride crosslinking on protein release, using microparticles with different internal protein distributions. Fluorescein isothiocyanate labelled bovine serum albumin is used as a model protein drug. The scaffolds display a homogeneous microparticle distribution,
and a reduction in pore size and percolation diameter with increased microparticle addition, although these values did not fall below those reported as necessary for cell invasion. The protein distribution within the microparticles, near the surface or more deeply located within the microparticles, was important in determining the release profile and effect of crosslinking, as the surface
was affected by the carbodiimide crosslinking reaction applied to the scaffold. Crosslinking of microparticles with a high proportion of protein at the surface caused both a reduction and delay in protein release. Protein located within the bulk of the microparticles, was protected from the crosslinking reaction and no delay in the overall release profile was seen
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Extracellular free water and glutathione in first-episode psychosis-a multimodal investigation of an inflammatory model for psychosis.
Evidence has been accumulating for an immune-based component to the etiology of psychotic disorders. Advancements in diffusion magnetic resonance imaging (MRI) have enabled estimation of extracellular free water (FW), a putative biomarker of neuroinflammation. Furthermore, inflammatory processes may be associated with altered brain levels of metabolites, such as glutathione (GSH). Consequently, we sought to test the hypotheses that FW is increased and associated with decreased GSH in patients with first-episode schizophrenia (SZ) compared with healthy controls (HC). SZ (n = 36) and HC (n = 40) subjects underwent a multi-shell diffusion MRI scan on a Siemens 3T scanner. 1H-MR spectroscopy data were acquired using a GSH-optimized MEGA-PRESS editing sequence and GSH/creatine ratios were calculated for DLPFC (SZ: n = 33, HC: n = 37) and visual cortex (SZ: n = 29, HC: n = 35) voxels. Symptoms and functioning were measured using the SANS, SAPS, BPRS, and GSF/GRF. SZ demonstrated significantly elevated FW in whole-brain gray (p = .001) but not white matter (p = .060). There was no significant difference between groups in GSH in either voxel. However, there was a significant negative correlation between DLPFC GSH and both whole-brain and DLPFC-specific gray matter FW in SZ (r = -.48 and -.47, respectively; both p < .05), while this relationship was nonsignificant in HC and in both groups in the visual cortex. These data illustrate an important relationship between a metabolite known to be important for immune function-GSH-and the diffusion extracellular FW measure, which provides additional support for these measures as neuroinflammatory biomarkers that could potentially provide tractable treatment targets to guide pharmacological intervention
Direct Probing of Vibrational Interactions in UiO-66 Polycrystalline Membranes with Femtosecond Two-Dimensional Infrared Spectroscopy
[Image: see text] UiO-66 is a benchmark metal–organic framework that holds great promise for the design of new functional materials. In this work, we perform two-dimensional infrared measurements on polycrystalline membranes of UiO-66 grown on c-sapphire substrates. We study the symmetric and antisymmetric stretch vibrations of the carboxylate groups of the terephthalate linker ions and find that these vibrations show a rapid energy exchange and a collective vibrational relaxation with a time constant of 1.3 ps. We also find that the symmetric vibration of the carboxylate group is strongly coupled to a vibration of the aromatic ring of the terephthalate ion. We observe that the antisymmetric carboxylate vibrations of different terephthalate linkers show rapid resonant (Förster) energy transfer with a time constant of ∼1 ps
Spatio-temporal correlations can drastically change the response of a MAPK pathway
Multisite covalent modification of proteins is omnipresent in eukaryotic
cells. A well-known example is the mitogen-activated protein kinase (MAPK)
cascade, where in each layer of the cascade a protein is phosphorylated at two
sites. It has long been known that the response of a MAPK pathway strongly
depends on whether the enzymes that modify the protein act processively or
distributively: distributive mechanism, in which the enzyme molecules have to
release the substrate molecules in between the modification of the two sites,
can generate an ultrasensitive response and lead to hysteresis and bistability.
We study by Green's Function Reaction Dynamics, a stochastic scheme that makes
it possible to simulate biochemical networks at the particle level and in time
and space, a dual phosphorylation cycle in which the enzymes act according to a
distributive mechanism. We find that the response of this network can differ
dramatically from that predicted by a mean-field analysis based on the chemical
rate equations. In particular, rapid rebindings of the enzyme molecules to the
substrate molecules after modification of the first site can markedly speed up
the response, and lead to loss of ultrasensitivity and bistability. In essence,
rapid enzyme-substrate rebindings can turn a distributive mechanism into a
processive mechanism. We argue that slow ADP release by the enzymes can protect
the system against these rapid rebindings, thus enabling ultrasensitivity and
bistability
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