Выращивание ремонтного молодняка кур при использовании пробиотических препаратов «Бацелл» и «Моноспорин»

Применение пробиотических препаратов с первых дней жизни цыплят позволит получить в дальнейшем здоровую птицу с высокой реализацией генетического потенциала

La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

15 avril 18731873/04/15 (N2534)

Additional file 3: Table S3. of Analysis of the DNA methylation level of cancer-related genes in colorectal cancer and the surrounding normal mucosa

Analysis of sensitivity and specificity of cancer-related genes

Establishment of stable <i>TET1</i> knockdown CRC cells.

<p>(A) Summaries of <i>TET1</i> expression in CIMP-high (CIMP-H), CIMP-low (CIMP-L) and CIMP-negative (CIMP-N) CRCs in TCGA datasets. (B) Results of RT-qPCR analyses of <i>TET1</i> in the indicated CRC cell lines and normal colonic tissues. (C) RT-qPCR analysis of <i>TET1</i> in control clones and stable <i>TET1</i> knockdown clones of the indicated CRC cells. ***<i>P</i> < 0.001 (one-way ANOVA with post hoc tests). (D) Dot blot analysis of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) in the indicated clones. Results using a control IgG are shown below as loading controls. (E) Bisulfite pyrosequencing analysis of repetitive elements in the indicated clones.</p

Association between DNA methylation changes and gene expression in <i>TET1</i> knockdown Colo320DM cells.

<p>(A) Venn diagram for genes with altered DNA methylation and genes with altered expression in <i>TET1</i> knockdown cells. (B) Heatmap of the expression of the 10 selected genes in (A). (C) Heatmap of the Infinium BeadChip probes for the 10 selected genes.</p

Genes with altered methylation and expression in TET1 knockdown Colo320DM cells.

<p>Genes with altered methylation and expression in TET1 knockdown Colo320DM cells.</p

Diminished effects of 5-aza-dC (Aza) in <i>TET1</i>-depleted HCT116 cells.

<p>(A) Analysis of DNA methylation (upper panel) and expression of representative tumor suppressor genes (lower panel) in control and <i>TET1</i> knockdown HCT116 cells treated with or without Aza. Methylation levels at 2 CpG sites in <i>DKK3</i>, 3 CpG sites in <i>SFRP1</i>, 4 CpG sites in <i>SFRP2</i> or 3 CpG sites in <i>SOX17</i> were analyzed using bisulfite pyrosequencing, and averages of respective genes are shown (upper panel). RT-qPCR results are shown relative to the expression levels in the Aza-treated CONT1 clone, which was assigned a value of 100 (lower panel). (B) Heatmap showing the expression of genes affected by Aza in HCT116 cells. Genes whose expression was altered after Aza in control clones were selected, and hierarchical clustering was performed. (C) Box plot showing the expression levels of genes upregulated by Aza. ***<i>P</i> < 0.001 (one-way ANOVA with post hoc tests). (D) Box plot showing the expression levels of genes downregulated by Aza. ***<i>P</i> < 0.001 (one-way ANOVA with post hoc tests).</p

Changes in DNA methylation induced by <i>TET1</i> depletion in Colo320DM cells.

<p>(A) Box plots showing the β-values of all probe sets from an Infinium HumanMethylation450 BeadChip for Colo320DM control or <i>TET1</i> knockdown clones. (B) Numbers of probe sets on an Infinium BeadChip with the indicated β-values in control and <i>TET1</i> knockdown clones. (C) Density plots showing BeadChip results for control and <i>TET1</i> knockdown clones. Shown are averages of three independent clones each. (D) Heatmap of the BeadChip probe sets differentially methylated between control and <i>TET1</i> knockdown clones. (E) Box plots showing the β-values of differentially methylated probes in the indicated clones. <i>P</i> < 0.001 (one-way ANOVA). (F) Genomic locations of the differentially methylated probes. (G) Box plots showing the expression levels of all genes or genes associated with differentially methylated probes (selected genes) in the indicated clones. <i>P</i> < 0.001 (one-way ANOVA).</p

Analysis of DNA methylation and expression of representative genes in Colo320DM cells.

<p>(A) Diagram of the Infinium BeadChip results around the transcription start site (TSS) of <i>HTATIP2</i> in control and <i>TET1</i> knockdown Colo320DM cells. (B) Infinium BeadChip results around the TSS of <i>HAS2</i>. The region analyzed with bisulfite sequencing is indicated on the top. (C) Bisulfite sequencing analysis of <i>HAS2</i>. Open and filled circles represent unmethylated and methylated CpG sites, respectively. (D) RT-qPCR analysis of <i>HAS2</i>. (E) Infinium BeadChip results around the first exon and intron of <i>GRB10</i>. (F) Bisulfite sequencing analysis of the intragenic CpG island of <i>GRB10</i>. (G) RT-qPCR analysis of <i>GRB10</i>.</p

Additional file 1 of Downregulation of SMOC1 is associated with progression of colorectal traditional serrated adenomas

Supplementary Material 1