21 research outputs found
Studies on phosphatidylserine by tandem quadrupole and multiple stage quadrupole ion-trap mass spectrometry with electrospray ionization: Structural characterization and the fragmentation processes
RFP is a DNA binding protein associated with the nuclear matrix.
We reported that the RFP gene encodes a protein with putative zinc finger domains and was involved in the activation of the ret proto-oncogene. To further characterize the RFP protein, we developed a polyclonal antibody against the product synthesized from a fragment of the RFP cDNA expressed in Escherichia coli. Western blot analysis showed that RFP was identified as a 58 kDa protein in cell lysates from four human and rodent cell lines and from mouse testis. In addition, a unique 68 kDa protein was detected in the testis. Using AH7974 (rat ascites hepatoma) and Raji (human Burkitt lymphoma) cells, we demonstrated strong association of RFP with the nuclear matrix. Furthermore, RFP solubilized from the nuclear matrix had DNA-binding activity although it appears to bind more preferentially to double-stranded DNA than to single-stranded DNA. These results thus suggest that RFP may play a role in molecular processes which occur in the nuclear matrix
A pre-operational variational data assimilation system for a non-hydrostatic model at the Japan Meteorological Agency: Formulation and preliminary results
Role of Δ9 desaturase activity in the maintenance of high levels of monoenoic fatty acids in hepatoma cultured cells
Individual profiles of free ceramide species and the constituent ceramide species of sphingomyelin and neutral glycosphingolipid and their alteration according to the sequential changes of environmental oxygen content in human colorectal cancer Caco-2 cells
Ca2+-induced reversible translocation of phospholipase A2 between the cytosol and the membrane fraction of rat liver macrophages
Inhibitory Effects of Cholesterol Derivatives on DNA Polymerase and Topoisomerase Activities, and Human Cancer Cell Growth
Progress of the ITER central solenoid model coil programme
The world's largest pulsed superconducting coil was successfully tested by charging up to 13 T and 46 kA with a stored energy of 640 MJ. The ITER central solenoid (CS) model coil and CS insert coil were developed and fabricated through an international collaboration, and their cooldown and charging tests were successfully carried out by international test and operation teams. In pulsed charging tests, where the original goal was 0.4 T/s up to 13 T, the CS model coil and the CS insert coil achieved ramp rates to 13 T of 0.6 T/s and 1.2 T/s, respectively. In addition, the CS insert coil was charged and discharged 10 003 times in the 13 T background field of the CS model coil and no degradation of the operational temperature margin directly coming from this cyclic operation was observed. These test results fulfilled all the goals of CS model coil development by confirming the validity of the engineering design and demonstrating that the ITER coils can now be constructed with confidence