緊張性振動反射が動作学習に与える影響 : 脳血管障害患者の肘関節伸展動作に着目して

本研究は脳血管障害患者8名を対象に麻痺側肘関節伸展動作の介入時に、緊張性振動反射（Tonic Vibration Reflex; TVR）を用いる事での動作学習への効果とその持続時間について明らかにする事を目的とした。研究デザインはクロスオーバー比較試験であり、条件①（肘関節屈曲伸展動作を自動運動で30回実施する条件）、条件②（条件①実施の際、肘関節伸展時に上腕三頭筋へのTVR を与えて実施する条件）の2条件である。各介入5、10分後にactive-ROMを測定して効果を検討した。介入5分後では両群共に条件②が条件①よりも有意に肘関節伸展角度が大きかった。介入10分後では両群共に条件間で有意差を示さなかった。このことからTVRを用いた機能的介入は即時効果としての促通効果に繋がる一方で、長期的な効果は認められなかった。これは本研究での介入期間が短すぎたことから、運動段階での内在的Feedbackに繋がらなかった事で忘却したものと考えられたThe purpose of this study was to examine effective use of TVR（Tonic Vibration Reflex） in motor training. Research Condition 1： The first condition consists of extending and flexing of a test subject\u27s elbow joint 30times without the use of TVR with a range of motion test of five and ten minutes. Research Condition 2： The second condition consists of using TVR on the triceps of the test subject\u27s elbow joint before extending and flexing the test subject\u27s elbow joint again 30times with a range of motion test of five and ten minutes. Results： After completing both tests, results shows that there is a significant and visible difference of the extension angle of the elbow joint while using TVR during the first five minutes of testing with both research conditions. However, there was not any significant or visible difference after the use of TVR after the ten minutes intervals of testing. Conclusion： TVR has an immediate effect in motor training. However, the TVR effect is only for a short amount of time. The possible reason for these results may be the short amount of time that was used to conduct the testing

Application of apolipoprotein E-modified liposomal nanoparticles as a carrier for delivering DNA and nucleic acid in the brain

An innovative drug delivery technology is urgently needed to satisfy unmet medical needs in treating various brain disorders. As a fundamental carrier for plasmid DNA or nucleic acids, we developed a liposomal nanoparticle (multifunctional envelope-type nano device [MEND]) containing a proton-ionizable amino lipid (YSK-MEND). Here we report on the impact of apolipoprotein E (ApoE) modification on the function of YSK-MEND in terms of targeting brain cells. The cellular uptake and function of YSK-MEND encapsulating short interference RNA or plasmid DNA were significantly improved as a result of ApoE modification in mouse neuron-derived cell lines (Neuro-2a and CAD). Intracerebroventricular administration of ApoE-modified YSK-MEND (ApoE/YSK-MEND) encapsulating plasmid DNA also resulted in higher transgene expression in comparison with YSK-MEND that was not modified with ApoE. Moreover, observation of fluorescence-labeled ApoE/YSK-MEND and expression of mCherry (fluorescence protein) derived from plasmid DNA indicated that this carrier might be useful for delivering and conferring transgene expression in neural stem cells and/or neural progenitor cells. Thus, this system may be a useful tool for the treatment of neurodegenerative disease