Fgf-dependent glial cell bridges facilitate spinal cord regeneration in Zebrafish

Adult Zebrafish show a remarkable capacity to regenerate their spinal column after injury, an ability that stands in stark contrast to the limited repair that occurs within the mammalian CNS post-injury. The reasons for this interspecies difference in regenerative capacity remain unclear. Here we demonstrate a novel role for Fgf signaling during glial cell morphogenesis in promoting axonal regeneration after spinal cordinjury. Zebrafish glia are induced by Fgf signaling, to form anelongated bipolarmorphology that formsabridge between the two sides of the resected spinal cord, over which regenerating axons actively migrate. Loss of Fgf function inhibits formation of this "glial bridge" and prevents axon regeneration. Despite the poor potential for mammalian axonal regeneration, primate astrocytes activated by Fgf signaling adopt a similar morphology to that induced in Zebrafish glia. This suggests that differential Fgf regulation, rather than intrinsic cell differences, underlie the distinct responses of mammalian and Zebrafish glia to injury

A Cytochrome P450 Conserved in Insects Is Involved in Cuticle Formation

The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue

Figure 2 raw data

<div>Raw data for Figure 2 in manuscript Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein.<br></div><div><br></div

Fig1 Raw Data

Statistical analysis, and tracking data for Figure 1

Supplementary Figure 8

confocal microscopy image serie

Figs 4,Supplementary Figure 5 & 6

Tracking data and statistical analysi

Figure 4 Raw data

Raw data for Figure 4 in manuscript Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein.<div><br></div

Supplementary Figure 1

Confocal data sets of neb mutants and siblings at 2 and 6 days post fertilisatio

Figure 4 and S5_Figure raw data

Raw data for figure 4 and S5_Figure of manuscript Genetic compensation triggered by actin mutation prevents the muscle damage caused by loss of actin protein

Fig2 Raw Data

Confocal image series, gomori trichrome labelling, and statistical analysis of data in figure 2