Use of Recombinant Endolysin to Improve Accuracy of Group B Streptococcus Tests

Group B Streptococcus (GBS) causes serious neonatal infection via vertical transmission. The prenatal GBS screening test is performed at the late stage of pregnancy to avoid risks of infection. In this test, enrichment culture is performed, followed by GBS identification. Selective medium is used for the enrichment; however, Enterococcus faecalis, which is a potential contaminant in swab samples, can interfere with the growth of GBS. Such bacterial contamination can lead to false-negative results. Endolysin, a bacteriophage-derived enzyme, degrades peptidoglycan in the bacterial cell wall; it is a promising antimicrobial agent for selectively eliminating specific bacterial genera/species. In this study, we used the recombinant endolysin EG-LYS, which is specific to E. faecalis; the endolysin potentially enriched GBS in the selective culture. First, in the false-negative model (coculture of GBS and E. faecalis, which disabled GBS detection in the subsequent GBS identification test), EG-LYS treatment at 0.1 mg/ml improved GBS detection. Next, we used 548 vaginal swabs to test the efficacy of EG-LYS treatment in improving GBS detection. EG-LYS treatment (0.1 mg/ml) increased the GBS-positive ratio to 17.9%, compared to 15.7% in the control (phosphate-buffered saline [PBS] treatment). In addition, there were an increased number of GBS colonies under EG-LYS treatment in some samples. The results were supported by the microbiota analysis of the enriched cultures. In conclusion, EG-LYS treatment of the enrichment culture potentially improves the accuracy of the prenatal GBS screening test

Functional Fv fragment of an antibody specific for CD28: Fv-mediated co-stimulation of T cells

AbstractThe most predominant co-stimulation pathway, which is critical for T cell activation and proliferation, is the CD28-B7 pathway. The anti-CD28 monoclonal antibody (mAb) also provides a co-stimulatory signal to T cells. In order to construct a functional Fv fragment (complex of VH and VL domains) of anti-CD28 antibody using a bacterial expression system, cDNA encoding the variable regions of immunoglobulin from 15E8 hybridoma cells was cloned and expressed in Escherichia coli. The Fv fragment was obtained as a soluble protein from the periplasmic fraction and showed a binding pattern similar to parental IgG. The Fv fragment induced proliferation of peripheral blood mononuclear cells in the presence of anti-CD3 or anti-CD2 mAb and enhanced anti-tumor activity of anti-MUC1×anti-CD3 bispecific antibody when tested with lymphokine-activated killer cells with T cell phenotype. Thus, the anti-CD28 Fv fragment will be promising not only for the study of co-stimulation, but also for cancer immunotherapy

The core mass function in the Orion Nebula Cluster region : What Determines the Final Stellar Masses?

Funding: European Research Council (ERC) via the ERC Synergy Grant ECOGAL (grant 855130) (R.S.K.).Applying dendrogram analysis to the CARMA-NRO C18O (J = 1–0) data having an angular resolution of ∼8'', we identified 692 dense cores in the Orion Nebula Cluster region. Using this core sample, we compare the core and initial stellar mass functions in the same area to quantify the step from cores to stars. About 22% of the identified cores are gravitationally bound. The derived core mass function (CMF) for starless cores has a slope similar to Salpeter's stellar initial mass function (IMF) for the mass range above 1 M⊙, consistent with previous studies. Our CMF has a peak at a subsolar mass of ∼0.1 M⊙, which is comparable to the peak mass of the IMF derived in the same area. We also find that the current star formation rate is consistent with the picture in which stars are born only from self-gravitating starless cores. However, the cores must gain additional gas from the surroundings to reproduce the current IMF (e.g., its slope and peak mass), because the core mass cannot be accreted onto the star with 100% efficiency. Thus, the mass accretion from the surroundings may play a crucial role in determining the final stellar masses of stars.Peer reviewe

CARMA-NRO Orion survey: Unbiased survey of dense cores and core mass functions in Orion A

The mass distribution of dense cores is a potential key to understanding the process of star formation. Applying dendrogram analysis to the CARMA-NRO Orion C18O (J = 1–0) data, we identify 2342 dense cores, about 22% of which have virial ratios smaller than 2 and can be classified as gravitationally bound cores. The derived core mass function (CMF) for bound starless cores that are not associate with protostars has a slope similar to Salpeter's initial mass function (IMF) for the mass range above 1 M⊙, with a peak at ∼0.1 M⊙. We divide the cloud into four parts based on decl., OMC-1/2/3, OMC-4/5, L1641N/V380 Ori, and L1641C, and derive the CMFs in these regions. We find that starless cores with masses greater than 10 M⊙ exist only in OMC-1/2/3, whereas the CMFs in OMC-4/5, L1641N, and L1641C are truncated at around 5–10 M⊙. From the number ratio of bound starless cores and Class II objects in each subregion, the lifetime of bound starless cores is estimated to be 5–30 freefall times, consistent with previous studies for other regions. In addition, we discuss core growth by mass accretion from the surrounding cloud material to explain the coincidence of peak masses between IMFs and CMFs. The mass accretion rate required for doubling the core mass within a core lifetime is larger than that of Bondi–Hoyle accretion by a factor of order 2. This implies that more dynamical accretion processes are required to grow cores.This work was supported in part by The Graduate University for Advanced Studies, SOKENDAI, and JSPS KAKENHI grant No. 22J13587. Data analysis was carried out on the Multi-wavelength Data Analysis System operated by the Astronomy Data Center (ADC), National Astronomical Observatory of Japan. This work was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with NASA (80NM0018D0004). N.S. acknowledges support from the project GENESIS (ANR-16-CE92-0035-01/DFG1591/2-1), the project FEEDBACK (DLR, 50 OR 1916), and the SFB 956 (DFG). P.S. was partially supported by a Grant-in-Aid for Scientific Research (KAKENHI Number 18H01259) of the Japan Society for the Promotion of Science (JSPS). P.P. acknowledges support by the grant PID2020-115892GBI00 funded by MCIN/AEI/10.13039/501100011033.Peer reviewe

Residual Tensile Property of Plain Woven Jute Fiber/Poly(Lactic Acid) Green Composites during Thermal Cycling

This study investigated the residual tensile properties of plain woven jute fiber reinforced poly(lactic acid) (PLA) during thermal cycling. Temperature ranges of thermal cycling tests were 35–45 °C and 35–55 °C. The maximum number of cycles was 103 cycles. The quasi-static tensile tests of jute fiber, PLA, and composite were conducted after thermal cycling tests. Thermal mechanical analyses of jute fiber and PLA were conducted after thermal cycling tests. Results led to the following conclusions. For temperatures of 35–45 °C, tensile strength of composite at 103 cycles decreased 10% compared to that of composite at 0 cycles. For temperatures of 35–55 °C, tensile strength and Young’s modulus of composite at 103 cycles decreased 15% and 10%, respectively, compared to that of composite at 0 cycles. Tensile properties and the coefficient of linear expansion of PLA and jute fiber remained almost unchanged after thermal cycling tests. From observation of a fracture surface, the length of fiber pull out in the fracture surface of composite at 103 cycles was longer than that of composite at 0 cycles. Therefore, tensile properties of the composite during thermal cycling were decreased, probably because of the decrease of interfacial adhesion between the fiber and resin