321 research outputs found
Simulating Electron Transport and Synchrotron Emission in Radio Galaxies: Shock Acceleration and Synchrotron Aging in Axis-Symmetric Flows
We introduce a simple and economical but effective method for including
relativistic electron transport in multi-dimensional simulations of radio
galaxies. The method is designed to follow explicitly diffusive acceleration at
shocks, and, in smooth flows 2nd order Fermi acceleration plus adiabatic and
synchrotron cooling. We are able to follow both the spatial and energy
distributions of the electrons, so that direct synchrotron emission properties
can be modeled in time-dependent flows for the first time.
Here we present first results in the form of some axis-symmetric MHD
simulations of Mach 20 light jet flows. These show clearly the importance of
nonsteady terminal shocks that develop in such flows even when the jet inflow
is steady. As a result of this and other consequences of the fundamentally
driven character of jets, we find complex patterns of emissivities and
synchrotron spectra, including steep spectral gradients in hot spots, islands
of distinct spectra electrons within the lobes and spectral gradients coming
from the dynamical histories of a given flow element rather than from
synchrotron aging of the embedded electrons. In addition, spectral aging in the
lobes tends to proceed more slowly than one would estimate from regions of high
emissivity.Comment: 30 pages of Latex generated text plus 7 figures in gif format.
Accepted for publication in the Astrophysical Journal. High resolution
postscript figures available through anonymous ftp at
ftp://ftp.msi.umn.edu/pub/users/twj/RGje
Positive Regulation by GABABR1 Subunit of Leptin Expression through Gene Transactivation in Adipocytes
Background: The view that c-aminobutyric acid (GABA) plays a functional role in non-neuronal tissues, in addition to an inhibitory neurotransmitter role in the mammalian central nervous system, is prevailing, while little attention has been paid to GABAergic signaling machineries expressed by adipocytes to date. In this study, we attempted to demonstrate the possible functional expression of GABAergic signaling machineries by adipocytes. Methodology/Principal Findings: GABAB receptor 1 (GABABR1) subunit was constitutively expressed by mouse embryonic fibroblasts differentiated into adipocytes and adipocytic 3T3-L1 cells in culture, as well as mouse white adipose tissue, with no responsiveness to GABA BR ligands. However, no prominent expression was seen with mRNA for GABA BR2 subunit required for heteromeric orchestration of the functional GABABR by any adipocytic cells and tissues. Leptin mRNA expression was significantly and selectively decreased in adipose tissue and embryonic fibroblasts, along with drastically reduced plasma leptin levels, in GABA BR1-null mice than in wild-type mice. Knockdown by siRNA of GABA BR1 subunit led to significant decreases in leptin promoter activity and leptin mRNA levels in 3T3-L1 cells. Conclusions/Significance: Our results indicate that GABABR1 subunit is constitutively expressed by adipocytes to primarily regulate leptin expression at the transcriptional level through a mechanism not relevant to the function as a partner o
Bcl-2 protein family: Implications in vascular apoptosis and atherosclerosis
Apoptosis has been recognized as a central component in the pathogenesis of atherosclerosis, in addition to the other human pathologies such as cancer and diabetes. The pathophysiology of atherosclerosis is complex, involving both apoptosis and proliferation at different phases of its progression. Oxidative modification of lipids and inflammation differentially regulate the apoptotic and proliferative responses of vascular cells during progression of the atherosclerotic lesion. Bcl-2 proteins act as the major regulators of extrinsic and intrinsic apoptosis signalling pathways and more recently it has become evident that they mediate the apoptotic response of vascular cells in response to oxidation and inflammation either in a provocative or an inhibitory mode of action. Here we address Bcl-2 proteins as major therapeutic targets for the treatment of atherosclerosis and underscore the need for the novel preventive and therapeutic interventions against atherosclerosis, which should be designed in the light of molecular mechanisms regulating apoptosis of vascular cells in atherosclerotic lesions
Development of dihydropyrrolopyridinone-based PKN2/PRK2 chemical tools to enable drug discovery
The Protein Kinase N proteins (PKN1, PKN2 and PKN3) are Rho GTPase effectors. They are involved in several biological processes such as cytoskeleton organization, cell mobility, adhesion, and cell cycle. Recently PKNs have been reported as essential for survival in several tumor cell lines, including prostate and breast cancer. Here, we report the development of dihydropyrrolopyridinone-based inhibitors for PKN2 and its closest homologue, PKN1, and their associated structure–activity relationship (SAR). Our studies identified a range of molecules with high potency exemplified by compound 8 with Ki = 8 nM for PKN2 and 14x selectivity over PKN1. Membrane permeability and target engagement for PKN2 were assessed by a NanoBRET cellular assay. Importantly, good selectivity across the wider human kinome and other kinase family members was achieved. These compounds provide strong starting points for lead optimization to PKN1/2 development compounds
A Potassium Metal-Organic Framework based on Perylene- 3,4,9,10-tetracarboxylate as Sensing Layer for Humidity Actuators
We have synthesized a novel three-dimensional metal-organic-framework (MOF) based on the
perylene-3,4,9,10-tetracarboxylate linker and potassium as metallic centre. We report the formation
of this K-based MOF using conventional routes with water as solvent. This material displays intense
green photoluminescence at room temperature, and displays an aggregation dependent quenching.
Correlation of the optical properties with the crystalline packing was confirmed by DFT calculations. We
also demonstrate its potential to build humidity actuators with a reversible and reproducible response,
with a change of 5 orders of magnitudes in its impedance at about 40% relative humidity (RH). This
3D-MOF is based on an interesting perylene derivative octadentate ligand, a moiety with interesting
fluorescent properties and known component in organic semiconductors. To the best of our knowledge,
this is the first time to build such a printed and flexible actuator towards humidity with a reversible
response, enabling precise humidity threshold monitoring.This work was supported by the Junta de Andalucía (FQM-1484, and FQM-195). Red Guipuzcoana de Ciencia,
Tecnología e Innovación (OF188/2017) and University of the Basque Country (GIU14/01, EHUA16/32). BB
acknowledges funding by RyC-2012–10381 contract and computational resources provided by the RES and
Alhambra supercomputing facilities. This work was also supported by the German Research Foundation (DFG)
and the Technical University of Munich within the Open Access Publishing Funding Programme
An Earth-sized Planet around an M5 Dwarf Star at 22 pc
We report on the discovery of an Earth-sized transiting planet (R p = 1.015 ± 0.051 R ⊕) in a P = 4.02 day orbit around K2-415 (EPIC 211414619), an M5V star at 22 pc. The planet candidate was first identified by analyzing the light-curve data obtained by the K2 mission, and it is here shown to exist in the most recent data from TESS. Combining the light curves with the data secured by our follow-up observations, including high-resolution imaging and near-infrared spectroscopy with IRD, we rule out false-positive scenarios, finding a low false-positive probability of 2 × 10−4. Based on IRD’s radial velocities of K2-415, which were sparsely taken over three years, we obtain a planet mass of 3.0 ± 2.7 M ⊕ (M p < 7.5 M ⊕ at 95% confidence) for K2-415b. Being one of the lowest-mass stars (≈0.16 M ⊙) known to host an Earth-sized transiting planet, K2-415 will be an interesting target for further follow-up observations, including additional radial velocity monitoring and transit spectroscopy
In vitro antibacterial activity of some plant essential oils
BACKGROUND: To evaluate the antibacterial activity of 21 plant essential oils against six bacterial species. METHODS: The selected essential oils were screened against four gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris) and two gram-positive bacteria Bacillus subtilis and Staphylococcus aureus at four different concentrations (1:1, 1:5, 1:10 and 1:20) using disc diffusion method. The MIC of the active essential oils were tested using two fold agar dilution method at concentrations ranging from 0.2 to 25.6 mg/ml. RESULTS: Out of 21 essential oils tested, 19 oils showed antibacterial activity against one or more strains. Cinnamon, clove, geranium, lemon, lime, orange and rosemary oils exhibited significant inhibitory effect. Cinnamon oil showed promising inhibitory activity even at low concentration, whereas aniseed, eucalyptus and camphor oils were least active against the tested bacteria. In general, B. subtilis was the most susceptible. On the other hand, K. pneumoniae exhibited low degree of sensitivity. CONCLUSION: Majority of the oils showed antibacterial activity against the tested strains. However Cinnamon, clove and lime oils were found to be inhibiting both gram-positive and gram-negative bacteria. Cinnamon oil can be a good source of antibacterial agents
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