Characterization of Mouse Tissue Kallikrein 5

Mouse tissue kallikreins (Klks) are members of a large, multigene family consisting of 37 genes, 26 of which can code for functional proteins. Mouse tissue kallikrein 5 (KIk5) has long been thought to be one of these functional genes, but the gene product, mK5, has not been isolated and characterized. In the present study, we prepared active recombinant mK5 using an Escherichia coli expression system, followed by column chromatography. We then determined the biochemical and enzymatic properties of purified mK5. mK5 had trypsin-like activity for Arg at the P1 position, and its activity was inhibited by typical serine protease inhibitors. mK5 degraded gelatin, fibronectin, collagen type IV, high-molecular-weight kininogen, and insulin-like growth factor binding protein-3. Our data suggest that mK5 may be implicated in the process of extracellular matrix remodeling

Scale Dependence of Halo Bispectrum from Non-Gaussian Initial Conditions in Cosmological N-body Simulations

We study the halo bispectrum from non-Gaussian initial conditions. Based on a set of large $N$-body simulations starting from initial density fields with local type non-Gaussianity, we find that the halo bispectrum exhibits a strong dependence on the shape and scale of Fourier space triangles near squeezed configurations at large scales. The amplitude of the halo bispectrum roughly scales as $f_nl^2$. The resultant scaling on the triangular shape is consistent with that predicted by Jeong & Komatsu based on perturbation theory. We systematically investigate this dependence with varying redshifts and halo mass thresholds. It is shown that the $f_nl$ dependence of the halo bispectrum is stronger for more massive haloes at higher redshifts. This feature can be a useful discriminator of inflation scenarios in future deep and wide galaxy redshift surveys.Comment: 27 pages, 10 figures; revised argument in section 6, added appendix C, JCAP accepted versio

Optimal Location of Two Laser-interferometric Detectors for Gravitational Wave Backgrounds at 100 MHz

Recently, observational searches for gravitational wave background (GWB) have been developed and given constraints on the energy density of GWB in a broad range of frequencies. These constraints have already resulted in the rejection of some theoretical models of relatively large GWB spectra. However, at 100 MHz, there is no strict upper limit from direct observation, though an indirect limit exists due to He4 abundance due to big-bang nucleosynthesis. In our previous paper, we investigated the detector designs that can effectively respond to GW at high frequencies, where the wavelength of GW is comparable to the size of a detector, and found that the configuration, a so-called synchronous-recycling interferometer is best at these sensitivity. In this paper, we investigated the optimal location of two synchronous-recycling interferometers and derived their cross-correlation sensitivity to GWB. We found that the sensitivity is nearly optimized and hardly changed if two coaligned detectors are located within a range 0.2 m, and that the sensitivity achievable in an experiment is far below compared with the constraint previously obtained in experiments.Comment: 17 pages, 6 figure

Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation

Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets

The 2006 November outburst of EG Aquarii: the SU UMa nature revealed

We report time-resolved CCD photometry of the cataclysmic variable EG Aquarii during the 2006 November outburst During the outburst, superhumps were unambiguously detected with a mean period of 0.078828(6) days, firstly classifying the object as an SU UMa-type dwarf nova. It also turned out that the outburst contained a precursor. At the end of the precursor, immature profiles of humps were observed. By a phase analysis of these humps, we interpreted the features as superhumps. This is the second example that the superhumps were shown during a precursor. Near the maximum stage of the outburst, we discovered an abrupt shift of the superhump period by ${\sim}$ 0.002 days. After the supermaximum, the superhump period decreased at the rate of $\dot{P}/P$=$-8.2{\times}10^{-5}$, which is typical for SU UMa-type dwarf novae. Although the outburst light curve was characteristic of SU UMa-type dwarf novae, long-term monitoring of the variable shows no outbursts over the past decade. We note on the basic properties of long period and inactive SU UMa-type dwarf novae.Comment: 9 pages, 7 figures, accepted for PAS

Ion cyclotron resonance heating system in the RT-1 magnetospheric plasma

We have developed an ion cyclotron resonance frequency (ICRF) heating system for the Ring Trap 1 (RT-1) magnetospheric device. We excite slow waves from the polar region of the dipole magnetic field. The target helium plasma is produced by electron cyclotron heating. The electrons comprise high-temperature (>10 keV) and low-temperature (<100 eV) components with both typically exhibiting densities of the same order of magnitude. The ICRF heating causes an increase in the ion temperatures and toroidal flow velocities in the core plasma region. We observe appreciable temperature differences between the different ion species (main He+ and impurity C2+), suggesting a strong influence of the charge-exchange loss, which caused the bulk ions to remain relatively cold (~20 eV) compared to the impurity ions (~40 eV). By developing an electro-optical measurement system, we have measured the local wave electric field in the plasma

Signal Fluctuation Sensitivity: An Improved Metric for Optimizing Detection of Resting-State fMRI Networks

Task-free connectivity analyses have emerged as a powerful tool in functional neuroimaging. Because the cross-correlations that underlie connectivity measures are sensitive to distortion of time-series, here we used a novel dynamic phantom to provide a ground truth for dynamic fidelity between blood oxygen level dependent (BOLD)-like inputs and fMRI outputs. We found that the de facto quality-metric for task-free fMRI, temporal signal to noise ratio (tSNR), correlated inversely with dynamic fidelity; thus, studies optimized for tSNR actually produced time-series that showed the greatest distortion of signal dynamics. Instead, the phantom showed that dynamic fidelity is reasonably approximated by a measure that, unlike tSNR, dissociates signal dynamics from scanner artifact. We then tested this measure, signal fluctuation sensitivity (SFS), against human resting-state data. As predicted by the phantom, SFS—and not tSNR—is associated with enhanced sensitivity to both local and long-range connectivity within the brain's default mode network