247 research outputs found

    PCR random mutagenesis into Escherichia coli serine acetyltransferase: isolation of the mutant enzymes that cause overproduction of L-cysteine and L-cystine due to the desensitization to feedback inhibition

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    AbstractPCR random mutagenesis in the cysE gene encoding Escherichia coli serine acetyltransferase was employed to isolate the mutant enzymes that, due to a much less feedback inhibition by L-cysteine, cause overproduction of L-cysteine and L-cystine in the recombinant strains. The L-cysteine auxotrophic and non-utilizing E. coli strain was transformed with plasmids having the altered cysE genes. Then, several transformants overproducing L-cysteine were selected by detecting the halo formation of the L-cysteine auxotroph. The production test of amino acids and analysis of the catalytic property on the mutant enzymes suggest that the carboxy-terminal region of serine acetyltransferase plays an important role in the desensitization to feedback inhibition and the high level production of L-cysteine and L-cystine

    A Discovery of a Peculiar Pulsar in the Small Magellanic Cloud

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    We report on a peculiar X-ray binary pulsar IKT1 = RXJ0047.3-7312 observed with XMM-Newton in Oct. 2000. The X-ray spectrum is described by a two-component spectrum. The hard component has a broken power-law with respective photon indices of 0.2 and 1.8, below and above the break energy at 5.8 keV. The soft component can be modeled by a blackbody of kT = 0.6 keV. The X-ray flux shows a gradual decrease and periodic variations of about 4000 s. The averaged flux in 0.7-10.0 keV is 2.9x10^-12 ergs/cm^2/s, which is ~10 times brighter than that in a ROSAT observation in Nov. 1999. In addition to the 4000-s variation, we found coherent pulsations of 263 +/- 1 s. These discoveries strengthen the Be/X-ray binary scenario proposed by the ROSAT and ASCA observations on this source, and confirm that most of the hard sources in the Small Magellanic Cloud are X-ray binary pulsars. A peculiar property of this XBP is that the coherent pulsations are found only in the soft component, and the folded light curve shows a flat top shape with a sharp dip. We discuss the nature of this XBP focusing on the peculiar soft component.Comment: 5 pages, 5figures, Accepted for publication in PAS

    Sensorless parameter estimation of electromagnetic transducer considering eddy currents

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    This paper presents a method of estimating the parameters of an electromagnetic transducer without sensors. The proposed method utilizes the measured admittance of the electromagnetic transducer, and therefore position, velocity, and/or acceleration sensors are not necessary in this framework. Novel impedance models are proposed based on the basic physical principles of electromagnetics; in particular, the effect of eddy currents has been included in these proposed models. The validity of the proposed estimation method and models was experimentally demonstrated by comparing the parameter estimation and vibration control capabilities of the proposed models with three conventional models. © 2017 Elsevier LtdEmbargo period 12 month

    An Annotated List of Culicoides Biting Midges in Yaeyama Islands in the Southwestern Part of Japan (Diptera: Ceratopogonidae)

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    Species of Culicoides biting midges (Diptera: Ceratopogonidae) in Yaeyama Islands were listed. In total, 36 species were included in the list, in which C. palpifer Das Gupta and Ghosh was new to Japan and C. cylindratus Kitaoka, C. malayae Macfie and C. sasai Kitaoka were new to the islands. Taxonomic and biological notes for some species were also given
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