138 research outputs found
Intertextualitás, szöveg-kép és szöveg-grafikon kapcsolatok vizsgálata gazdasági témájú klaszterekben
Az egyenlő bánásmód elméleti kérdései a munkajog jogági besorolása vonatkozásában
A munkajog jogáig besorolását érintően számos hazai és nemzetközi tudományos vita van napirenden. Egyfelől a munkajogot a magánjog rendszerébe kategorizálhatjuk akkor, ha a munkaviszonyok létrejöttét vizsgáljuk. A munkaviszony jogalapja ugyanis kizárólag a munkaszerződés lehet, így a munkajog valamelyest illeszkedik a klasszikus magánjogba. Másfelől viszont a munkaviszony tartalmát nem csak a munkaszerződés, hanem több más norma képes alakítani. E normák jellemzően közjogi tartalommal bírnak, és mint munkajog úgynevezett közjogi elemei, a felek szerződési szabadságát igyekeznek korlátozni. A közjogi elemek létét ugyanis tipikusan azzal igazolja a jogalkotó, hogy a munkaviszonyban a felek között szubordináció van, így a magánjogban jellemző mellérendelt szerződéses egyensúly megbillent a munkáltató javára. A közjogi elemek jelenléte és így különösen az egyenlő bánásmód előírása e megbillent egyensúlyt kívánja helyreállítani a munkajogban oly módon, hogy korlátozza a felek közötti szerződéses szabadságot. A jelen írásunkban elsősorban azt vizsgáljuk, hogy a közjogi elemek jelenléte miatt a munkajog bír-e önálló jogági sajátossággal. Mindemellett pedig az egyenlő bánásmód alapelvére fókuszálva vizsgáljuk azt, hogy miként értelmezhető a diszkrimináció tilalma a munkajogban és a klasszikus magánjogban, valamint ezen általános magatartási követelmény alkalmas-e arra, hogy a felek közötti megbomlott egyensúlyt helyreállítsa. Végül pedig feltesszük azt a költői kérdést: ha az egyenlő bánásmód követelménye alkalmas az egyensúlyi állapot helyreállítására, akkor miért van szükség további közjogi elemekre a munkajogban
The anaerobic digestion of sheep manure in self-designed low-cost biogas reactor
One of the possible utilisation methods for organic wastes is anaerobe decomposition (fermentation). The main product of this process is biogas which is usually used for energy purposes due to its composition (mainly methane and carbon dioxide). The residual solid material after fermentation can be used as soil conditioner.
Lab-scale fermentation can be carried out using the “VDI 4630 – Fermentation of organic materials Characterisation of the substrate, sampling, collection of material data, fermentation tests” standard. Based on the conditions described in the standard, a small-scale low-budget reactor system were prepared. The temperature during the holding time was controlled with water bath and the gas production was determined with fluid displacement method. A peristaltic pump was used for the recirculation of the gas to mix the base material. Furthermore, the temperatures of the environment, the water baths and the inside of each reactor was automatically registered on a data collector
The effects of viral load on pseudorabies virus gene expression
<p>Abstract</p> <p>Background</p> <p>Herpesvirus genes are classified into distinct kinetic groups on the basis of their expression dynamics during lytic growth of the virus in cultured cells at a high, typically 10 plaque-forming units/cell multiplicity of infection (MOI). It has been shown that both the host response and the success of a pathogen are dependent on the quantity of particles infecting an organism. This work is a continuation of an earlier study <abbrgrp><abbr bid="B1">1</abbr></abbrgrp>, in which we characterized the overall expression of PRV genes following low-MOI infection. In the present study, we have addressed the question of whether viral gene expressions are dependent on the multiplicity of infection by comparing gene expressions under low and high-MOI conditions.</p> <p>Results</p> <p>In the present study, using a real-time RT-PCR assay, we address the question of whether the expression properties of the pseudorabies virus (PRV) genes are dependent on the number of virion particles infecting a single cell in a culture. Our analysis revealed a significant dependence of the gene expression on the MOI in most of these genes. Specifically, we found that most of the examined viral genes were expressed at a lower level at a low MOI (0.1) than at a high MOI (10) experiment in the early stage of infection; however, this trend reversed by six hour post-infection in more than half of the genes. Furthermore, in the high-MOI infection, several PRV genes substantially declined within the 4 to 6-h infection period, which was not the case in the low-MOI infection. In the low-MOI infection, the level of antisense transcript (AST), transcribed from the antiparallel DNA strand of the immediate-early 180 (<it>ie180</it>) gene, was comparable to that of <it>ie180 </it>mRNA, while in the high-MOI experiment (despite the 10 times higher copy number of the viral genome in the infected cells) the amount of AST dropped by more than two log values at the early phase of infection. Furthermore, our analysis suggests that adjacent PRV genes are under a common regulation. This is the first report on the effect of the multiplicity of infection on genome-wide gene expression of large DNA viruses, including herpesviruses.</p> <p>Conclusion</p> <p>Our results show a strong dependence of the global expression of PRV genes on the MOI. Furthermore, our data indicate a strong interrelation between the expressions of <it>ie180 </it>mRNA and AST, which determines the expression properties of the herpesvirus genome and possibly the replication strategy (lytic or latent infection) of the virus in certain cell types.</p
The Influence of Solvents and Colloidal Particles on the Efficiency of Molecular Antioxidants
The radical scavenging activity of three molecular antioxidants (trolox, rutin and ellagic acid) was investigated in different solvents with and without added polymer-based colloidal particles (SL-IP-2). Rutin and ellagic acid showed poor solubility in water, preventing the accurate measurement of the effective antioxidant concentration values, which were determined in ethanol/water (EtOH/H2O) mixtures. The presence of trolox and rutin changed neither the surface charge properties nor the size of SL-IP-2 in these solvents, while significant adsorption on SL-IP-2 was observed for ellagic acid leading to overcharging and rapid particle aggregation at appropriately high antioxidant concentrations in EtOH/H2O. The differences in the radical scavenging capacity of trolox and ellagic acid that was observed in homogeneous solutions using water or EtOH/H2O as solvents vanished in the presence of the particles. Rutin lost its activity after addition of SL-IP-2 due to the larger molecular size and lower exposure of the functional groups to the substrate upon interaction with the particles. The obtained results shed light on the importance of the type of solvent and particle–antioxidant interfacial effects on the radical decomposition ability of molecular antioxidants, which is of crucial importance in industrial processes involving heterogeneous systems
The anaerobic digestion of sheep manure in self-designed low-cost biogas reactor
One of the possible utilisation methods for organic wastes is anaerobe decomposition (fermentation). The main product of this process is biogas which is usually used for energy purposes due to its composition (mainly methane and carbon dioxide). The residual solid material after fermentation can be used as soil conditioner. Lab-scale fermentation can be carried out using the “VDI 4630 – Fermentation of organic materials Characterisation of the substrate, sampling, collection of material data, fermentation tests” standard. Based on the conditions described in the standard, a small-scale low-budget reactor system were prepared. The temperature during the holding time was controlled with water bath and the gas production was determined with fluid displacement method. A peristaltic pump was used for the recirculation of the gas to mix the base material. Furthermore, the temperatures of the environment, the water baths and the inside of each reactor was automatically registered on a data collector. Based on the gathered data, the system is applicable for biogas production from sheep manure. The produced biogas quantities were between 0.01-0.15 m 3 /kg TS and the methane content was 24-63 vol% during the experiment at various temperatures, using different inoculants
Revisit of solubility of oxytetracycline polymorphs. An old story in the light of new results
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