54 research outputs found
Pneumonia intersticial crônica em cães naturalmente infectados com Leishmania (Leishmania) chagasi: estudo histopatológico e morfométrico
Eighteen mongrel dogs of unknown age and naturally infected with Leishmania (Leishmania) chagasi, were obtained from the City Hall of Belo Horizonte, Brazil. Four dogs were used as control. Lung samples were obtained and immediately fixed in formalin. The histopathological picture of all lung tissue sections was a chronic and diffuse interstitial pneumonitis. The thickened inter-alveolar septa were characterized by the cellular exudate (mostly macrophages, lymphocytes and plasmocytes) associated with collagen deposition. Morphometric analysis showed greater septal thickness in the infected animals than in controls. In fact, the morphometric study of collagen stained with ammoniac silver confirmed a larger deposition of collagen in the infected animals. The parasitologic method was carried out during the study of the lesions on the slides. However, we did not observe any correlation between the histopathologic and morphometric data and the clinical status of the animals. We conclude that the pulmonary lesions observed in all naturally infected dogs were correlated with the disease and that the morphometric method used was satisfactory for the analysis of septal thickness and of increased collagen deposition, confirming the presence of fibrosis.Neste estudo, foram utilizados dezoito cães naturalmente infectados com Leishmania (Leishmania) chagasi, sem raça e idade definidos, cedidos pelo Centro de Contole de Zoonoses da Cidade de Belo Horizonte. Quatro cães foram utilizados como controle. Durante a necropsia, amostras do pulmão foram coletadas, e imediatamente fixadas em formalina. A histopatologia mostrou principalmente uma pneumonite intersticial crônica, produtiva, difusa e intensa. Os septos inter-alveolares espessados, eram constituÃdos principalmente por um infiltrado de células inflamatórias, em sua maioria, macrófagos, linfócitos e plasmócitos, além de fibroblastos e fibras colágenas. A análise morfométrica do espessamento septal mostrou diferença significativa entre animais infectados e controle. De fato, a análise morfométrica do colágeno, corado pela prata, confirmou um aumento na deposição de colágeno em todos os animais infectados. O exame parasitológico foi ralizado durante a análise histopatológica, em todos os casos estudados. Contudo neste trabalho, não observamos qualquer correlação entre os achados histopatológicos e morfométricos quando relacionados aos cães em diferentes formas clÃnicas. ConcluÃmos que as lesões, encontradas nos cães naturalmente infectados, tem correlação com a doença, e que o método de análise morfométrica utilizado apresentou resultados satisfatórios quanto à análise do espessamento septal e ao aumento da deposição de colágeno, confirmando a fibrose
Comparison of paraffin-embedded skin biopsies from different anatomical regions as sampling methods for detection of Leishmania infection in dogs using histological, immunohistochemical and PCR methods
BACKGROUND: We compared skin biopsy samples from different anatomical regions for detecting Leishmania in dogs, using histological (HE), immunohistochemical (IHC) and polymerase chain reaction (PCR) techniques. RESULTS: The sensitivity was 82.8 percent for PCR, 62.1 percent for IHC and 44.8 percent for HE. These methods do not appear to depend on the clinical status of the animal or the anatomical source of the skin sample; there is no "best region" for any method. However, PCR was more effective than IHC and HE for ear and nose skin samples whereas IHC was better than HE for nose samples. There was weak agreement between PCR and HE for all tissue samples; good agreement between PCR and IHC for ear and abdomen samples, and weak agreement for nose; and optimal agreement between IHC and HE for ear and abdomen and good agreement for nose samples. CONCLUSION: The PCR on ear skin could be the best procedure for diagnosing canine visceral leishmaniasis. The good agreement between PCR and IHC indicates that IHC can be used as an alternative method. Finally, tissue samples from ears, nose and abdomen, particularly ears and nose, are potentially useful for diagnosing canine visceral leishmaniasis independently of the animal's clinical status
A potential link among antioxidant enzymes, histopathology, and trace elements in canine visceral leishmaniasis.
Canine visceral leishmaniasis (CVL) is a severe and fatal systemic chronic inflammatory
disease. We investigated the alterations in, and potential associations among,
antioxidant enzymes, trace elements and histopathology in CVL. Blood and tissue
levels of Cu-Zn superoxide dismutase, catalase and glutathione peroxidase were
measured in mixed-breed dogs naturally infected with Leishmania infantum chagasi,
symptomatic (n = 19) and asymptomatic (n = 11). Serum levels of copper, iron, zinc,
selenium and nitric oxide, and plasma lipid peroxidation were measured. Histological
and morphometric analyses were conducted of lesions in liver, spleen and lymph
nodes. We found lower blood catalase and glutathione peroxidase activity to be correlated
with lower iron and selenium respectively. However, higher activity of Cu-
Zn superoxide dismutase was not correlated with the increase in copper and
decreased in zinc observed in infected animals compared to controls. Organ tissue
was characterized by lower enzyme activity in infected dogs than in controls, but
this was not correlated with trace elements. Lipid peroxidation was higher in symptomatic
than in asymptomatic and control dogs and was associated with lesions such
as chronic inflammatory reaction, congestion, haemosiderin and fibrosis. Systemic
iron deposition was observed primarily in the symptomatic dogs showing a higher
tissue parasite load. Dogs with symptomatic CVL displayed enhanced LPO and Fe
tissue deposition associated with decreased levels of antioxidant enzymes. These
results showed new points in the pathology of CVL and might open new treatment
perspectives associated with antioxidants and the role of iron in the pathogenesis of
CVL
Schistosoma mansoni granuloma in late evolutive phase, in a case of tumoral form in man
Ectopic Cutaneous Schistosomiasis mansoni in the Sacral Region
The authors report one case of late cutaneous Schistosomiasis mansoni in a biopsy of a skin lesion in the sacral region in a 51-year-old female living in Contagem, Minas Gerais. The patient was treated successfully with oxamniquine (Mansil®)
Chronic interstitial pneumonitis in dogs naturally infected with Leishmania (Leishmania) chagasi: a histopathological and morphometric study
Eighteen mongrel dogs of unknown age and naturally infected with Leishmania (Leishmania) chagasi, were obtained from the City Hall of Belo Horizonte, Brazil. Four dogs were used as control. Lung samples were obtained and immediately fixed in formalin. The histopathological picture of all lung tissue sections was a chronic and diffuse interstitial pneumonitis. The thickened inter-alveolar septa were characterized by the cellular exudate (mostly macrophages, lymphocytes and plasmocytes) associated with collagen deposition. Morphometric analysis showed greater septal thickness in the infected animals than in controls. In fact, the morphometric study of collagen stained with ammoniac silver confirmed a larger deposition of collagen in the infected animals. The parasitologic method was carried out during the study of the lesions on the slides. However, we did not observe any correlation between the histopathologic and morphometric data and the clinical status of the animals. We conclude that the pulmonary lesions observed in all naturally infected dogs were correlated with the disease and that the morphometric method used was satisfactory for the analysis of septal thickness and of increased collagen deposition, confirming the presence of fibrosis
Histopathological and parasitological investigations of ear healthy skin of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi
Although 90% of clinical cases of American
visceral leishmaniasis (AVL) occur in the northeastern
region of Brazil, the incidence of cases in recent years
has increased in southeastern states such as Minas Gerais
(MG), where the disease has been reported in several
cities, including Belo Horizonte, the state capital. Some
studies have shown a strong correlation between the
incidence of AVL and canine visceral leishmaniasis
(CVL) in Belo Horizonte. A study of 108 dogs with
parasite Leishmania chagasi detected by immunohistochemistry
in healthy ear skin was obtained from
two distinct geographical areas: 55 from a metropolitan
area of the municipality (Santa Luzia, MG) and 53 dogs
from a central area of Belo Horizonte. In parallel, a
group of 10 beagles were experimentally infected with
L. chagasi. Considering the clinical aspects of all
naturally infected dogs, symptomatic dogs were more
frequent than asymptomatic ones, especially animals
from the metropolitan area compared with the central
area (79.6% and 20.3%, respectively). A chronic exudate
was observed in the ear of 51 out of 55 dogs naturally
infected from the metropolitan area (92.7%) and 45 out
of 53 dogs naturally infected from the central area
(84.9%). Importantly, asymptomatic dogs from the
central area harbor more parasites in the skin than the
asymptomatic ones from the metropolitan area. In
addition, a profound difference was noted in the intensity
of the inflammatory reaction and parasite load in the
skin of experimental infected dogs
Histopathology and immunocytochemical study of type 3 and type 4 complement receptors in the liver and spleen of dogs naturally and experimentally infected with Leishmania (Leishmania) chagasi
Os objetivos deste trabalho visaram uma análise comparativa das alterações histopatológicas e da expressão de CR3 e CR4 no fÃgado e baço de cães natural e experimentalmente infectados com L. chagasi. As lesões histopatológicas fundamentais observadas principalmente nos cães naturalmente infectados foram: os granulomas epitelióides hepáticos, a hiperplasia e a hipertrofia das células de Kupffer, dos folÃculos de Malpighi e das células mononucleadas da polpa vermelha do baço. Os cortes de fÃgado e baço corados pela técnica de imunocitoquÃmica mostraram a presença dos antÃgenos CD11b,c\CD18 nos animais controles e infectados, sem diferenças qualitativas e quantitativas no fÃgado. Entretanto, no baço dos cães natural e experimentalmente infectados a expressão de CD 18 (subunidade beta2 da molécula comum aos leucócitos) foi sempre aumentada. Em leishmaniose a resposta imune celular é considerada a mais importante na resolução da doença. A expressão de CD11 b,c\CD 18 pelos macrófagos deve representar um papel central na resposta celular. Estes resultados indicam que tanto na leishmaniose visceral canina experimental ou natural existe uma diferença na ativação de CD 18, o qual deve exercer uma importante função na resposta imunológica na infecção por Leishmania chagasi.The objective of this study was to compare the histopathological changes and expression of CR3 and CR4 in the liver and spleen of dogs naturally and experimentally infected with L. chagasi. The basic histopathological lesions observed mainly in naturally infected dogs were: epithelioid hepatic granulomas, hyperplasia and hypertrophy of Kupffer cells, Malpigui follicles and mononucleated cells of the red pulp of the spleen. Sections from the liver and spleen by immunocytochemistry technique showed the presence of CD11b,c\CD 18 antigens in the control and infected animals and no qualitative or quantitative differences in the liver. Nevertheless, CD18 was always increased in the spleen of naturally and experimentally infected dogs. These results indicate that there is a difference in the activaton of CD 18 in both experimental and natural cases of canine visceral leishmaniasis that should play an important role in the immunological response to Leishmania chagasi infection
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