STUDIES ON ECHINOCOCCOSIS XX : PRELIMINARY OBSERVATIONS OF THE IN VIVO CULTIVATION OF LARVAL TISSUE OF ECHINOCOCCUS MULTILOCULARIS IN CULTURE-CHAMBER OF POROUS MEMBRANE

Echinococcal tissue consisted essentially of 300 to 500 scolices, a few germinal cells and small pieces of brood capsule, was introduced into the culture-chamber made of porous membrane of 0.45±0.02μ in pore-size, composed of cellulose ester, and silicon gum tube, 3.5^2π×7mm^3 in capacity. Three or 4 culture-chambers containing echinococcal tissue were inserted into abdominal cavity of a cotton rat, and the development of echinococcal tissue was observed morphologically in the course of time. The results obtained are as follows. On the 3rd day, vesicles which were lined by typical germinal cells and showed initial brood capsule formation filled up the space of the culture-chamber. On the 80th day, many mature brood capsules with scolices fully developed, daughter cysts and numerous calcareous corpuscles were present in the vesicles. The culture-chamber was full of vesicles with a remarkable number of brood capsules containing many scolices after 6 months. No multilocular vesiculation by exogenous budding was recognized

High-Flow Nasal Cannula Therapy in a Patient with Reperfusion Pulmonary Edema following Percutaneous Transluminal Pulmonary Angioplasty

A 62-year-old woman with Wolff-Parkinson-White syndrome was with recent worsening of dyspnea to New York Heart Association functional status Class III. The patient was diagnosed as having central type chronic thromboembolic pulmonary hypertension. By cardiac catheterization, her mean pulmonary artery pressure was 53 mmHg with total pulmonary resistance 2238 dynes·sec·cm−5. After medical therapies with tadalafil, furosemide, ambrisentan, beraprost, and warfarin were initiated, percutaneous transluminal pulmonary angioplasty (PTPA) was performed. Following PTPA, life-threating hypoxemia resulting from postoperative reperfusion pulmonary edema developed. High-flow nasal cannula therapy (HFNC) was applied, and 100% oxygen at 50 L/min of flow was required to keep oxygenation. HFNC was continued for 3 days, and the patient was discharged on 8th postoperative day with SpO2 of 97% on 3 L/min of oxygen inhalation. Because of the simplicity of the technique, the lower cost of equipment, and remarkable patient tolerance to the treatment, we speculate that HFNC can take over the post of noninvasive ventilation as first-line therapy for patients with acute respiratory failure