12 research outputs found
Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction
Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3Γ106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and Ξ±-smooth muscle actin (Ξ±-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of Ξ±-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. Β© Human stem cells institute, 2013
Comparison of different methods of rat hepatic stellate cells isolation, labeling and transplantation
Nowadays more and more attention is turned to hepatic stellate cells (HSC) and their role in liver regeneration. Nonetheless there are several methodological questions, for example, methods of HSC isolation, their labeling and ways of transplantation. In this work we compared two different methods of HSC isolation: collagenase-pronase liver perfusion with further Histodenz density gradient centrifugation and method of Seglen for isolation of hepatocytes associated with HSC. We also analyzed diverse methods of cells labeling: membrane fluorescent labels PKH26 and with gene of green fluorescent protein (GFP), that could be get into the cells by electroporation, with chemicals like TurboFect or by adenovirus. Then cells were transplanted into rats in two ways: into lien and into system of portal vein. According to our results, we able to conclude that collagenase-pronase liver perfusion with further cells gradient centrifugation in Histodenz is better for HSC isolation than method of Seglen, the most optimal method for cells labeling is with adenovirus, expressing the GFP gene, for HSC transplantation - Transplantation into system of portal vein. Β© Human stem cells institute, 2013
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΊΠΎΠ»Π»Π°ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ Π²Π΅Π½ΡΠΈΠ»ΡΡΠΈΠΈ Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ Ρ Π΄ΡΡΠ³ΠΈΠΌΠΈ ΡΠ°ΠΊΡΠΎΡΠ°ΠΌΠΈ Π½Π° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΡΠΎΡΠΌΠ°ΠΌΠΈ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° Π»Π΅Π³ΠΊΠΈΡ
Introduction. According to studies on the treatment of emphysema, the absence of CT signs of collateral ventilation correlates with the results of valvular blocking procedure, but there is not any data on the relationship between the signs of collateral ventilation and the results of bronchial valve treatment in destructive forms of pulmonary tuberculosis.Study goals: to study the effect of CT features of collateral ventilation in combination with other factors on the results of valvular bronchial blocking in patients with destructivepulmonaryΒ pathology in tuberculosis.Materials and methods. Π’he study is based on the results of evaluation of chest CT data of patients with various pathology of pulmonary tuberculosis before and after valvular blocking procedure.Results. Π‘orrelations between such characteristics as collateral ventilation, additional factors and valvular treatment effects have been determined, and prognostic models estimating the success of valve therapy have been constructed.Conclusion. Positive pleural integrity symptom is a predictor of lobe volume reduction after bronchial valve treatment. Lobe volume reduction during valve therapy is a prognostic factor for cavityβs closure.Β ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. ΠΠΎ Π΄Π°Π½Π½ΡΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π½ΡΡ
Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠΌΡΠΈΠ·Π΅ΠΌΡ, ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΊΠΎΠ»Π»Π°ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ Π²Π΅Π½ΡΠΈΠ»ΡΡΠΈΠΈ ΠΊΠΎΡΡΠ΅Π»ΠΈΡΡΠ΅Ρ ΡΒ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°ΠΌΠΈ ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ, Π½ΠΎ Π½Π΅Ρ Π½ΠΈΠΊΠ°ΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΎΒ ΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΊΠΎΠ»Π»Π°ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ Π²Π΅Π½ΡΠΈΠ»ΡΡΠΈΠΈ ΠΈΒ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°ΠΌΠΈ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ ΠΏΡΠΈ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΡΠΌΠ°Ρ
ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° Π»Π΅Π³ΠΊΠΈΡ
.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ½ΠΈΡ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΊΠΎΠ»Π»Π°ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ Π²Π΅Π½ΡΠΈΠ»ΡΡΠΈΠΈ Π²Β ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ ΡΒ Π΄ΡΡΠ³ΠΈΠΌΠΈ ΡΠ°ΠΊΡΠΎΡΠ°ΠΌΠΈ Π½Π°Β ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ ΡΒ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΡΒ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΡΠΎΡΠΌΠ°ΠΌΠΈ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° Π»Π΅Π³ΠΊΠΈΡ
.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈΒ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΎ Π½Π°Β ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°Ρ
ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ»ΠΎΠ³ΠΎΠΌ Π΄Π°Π½Π½ΡΡ
ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΎΡΠ³Π°Π½ΠΎΠ² Π³ΡΡΠ΄Π½ΠΎΠΉ ΠΏΠΎΠ»ΠΎΡΡΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΡΒ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠ²Π½ΡΠΌΠΈ ΡΠΎΡΠΌΠ°ΠΌΠΈ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° Π»Π΅Π³ΠΊΠΈΡ
Π΄ΠΎΒ ΠΈΒ ΠΏΠΎΡΠ»Π΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠ΅Π½Π΅Π½Ρ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΊΠΎΠ»Π»Π°ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ Π²Π΅Π½ΡΠΈΠ»ΡΡΠΈΠΈ, Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ ΡΠ°ΠΊΡΠΎΡΠ°ΠΌΠΈ ΠΈΒ ΡΡΡΠ΅ΠΊΡΠ°ΠΌΠΈ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ, ΠΏΠΎΡΡΡΠΎΠ΅Π½Ρ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠΎΠ΄Π΅Π»ΠΈ Π΄ΠΎΡΡΠΈΠΆΠ΅Π½ΠΈΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΡΠΈΠΌΠΏΡΠΎΠΌ ΡΠ΅Π»ΠΎΡΡΠ½ΠΎΡΡΠΈ ΠΏΠ»Π΅Π²ΡΡ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΎΠΌ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΡ ΠΎΠ±ΡΠ΅ΠΌΠ° Π΄ΠΎΠ»ΠΈ ΠΏΠΎΡΠ»Π΅ ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ. Π£ΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΠΎΠ±ΡΠ΅ΠΌΠ° Π΄ΠΎΠ»ΠΈ ΠΏΡΠΈ ΠΊΠ»Π°ΠΏΠ°Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΎΠ±Π»ΠΎΠΊΠ°ΡΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ Π·Π°ΠΊΡΡΡΠΈΡ ΠΏΠΎΠ»ΠΎΡΡΠΈ Π΄Π΅ΡΡΡΡΠΊΡΠΈΠΈ.
ΠΠ΅ΡΠΎΠ΄ Π·ΠΎΠ½Π΄ΠΎΠ²ΠΎΠΉ ΠΊΠΎΠ½ΡΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ Π»Π°Π·Π΅ΡΠ½ΠΎΠΉ ΡΠ½Π΄ΠΎΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΈΠ΄ΠΈΠΎΠΏΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ ΠΈΠ½ΡΠ΅ΡΡΡΠΈΡΠΈΠ°Π»ΡΠ½ΡΡ ΠΏΠ½Π΅Π²ΠΌΠΎΠ½ΠΈΠΉ
The diagnosis of idiopathic interstitial pneumonia (IIP) remains one of the most challenging issues in pulmonology. The article demonstrates the capabilities of the method of probe-based confocal laser endomicroscopy (pCLE) in diagnosing various forms of IIPs and provides comparisons with CT and the histological picture. According to the data obtained, it is difficult to distinguish most IIPs from each other using pCLE. Among the specific signs, a large number of alveolar cells in pCLE in a nonsmoking patient with desquamative interstitial pneumonia is not typical for other IIP in nonsmokers. Also, pCLE can be used to differentiate between IIP and other conditions, in particular, malignant lesions.ΠΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΠΈΠ΄ΠΈΠΎΠΏΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ½ΡΠ΅ΡΡΡΠΈΡΠΈΠ°Π»ΡΠ½ΡΡ
ΠΏΠ½Π΅Π²ΠΌΠΎΠ½ΠΈΠΉ (ΠΠΠ) ΠΎΡΡΠ°Π΅ΡΡΡ ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΡΠ»ΠΎΠΆΠ½ΡΡ
Π²ΠΎΠΏΡΠΎΡΠΎΠ² ΠΏΡΠ»ΡΠΌΠΎΠ½ΠΎΠ»ΠΎΠ³ΠΈΠΈ. Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΊΠΎΠ½ΡΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ Π»Π°Π·Π΅ΡΠ½ΠΎΠΉ ΡΠ½Π΄ΠΎΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΠΈ (ΠΠΠΠ) Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΎΡΠΌ ΠΠΠ, ΠΏΡΠΈΠ²Π΅Π΄Π΅Π½Ρ ΡΠΎΠΏΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΡ Ρ Π΄Π°Π½Π½ΡΠΌΠΈ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΈ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΉ. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ ΡΠΎΠΌ, ΡΡΠΎ Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²ΠΎ ΠΠΠ ΡΠ»ΠΎΠΆΠ½ΠΎ ΡΠ°Π·Π»ΠΈΡΠΈΡΡ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠΎΠ±ΠΎΠΉ ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΠΠΠΠ. ΠΠ· ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΌΠΎΠΆΠ½ΠΎ ΠΎΡΠΌΠ΅ΡΠΈΡΡ Π½Π°Π»ΠΈΡΠΈΠ΅ Π±ΠΎΠ»ΡΡΠΎΠ³ΠΎ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° Π°Π»ΡΠ²Π΅ΠΎΠ»ΡΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΡΠΈ ΠΠΠΠ Ρ Π½Π΅ΠΊΡΡΡΡΠ΅ΠΉ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠΈ Ρ Π΄Π΅ΡΠΊΠ²Π°ΠΌΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΡΡΡΠΈΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΠ½Π΅Π²ΠΌΠΎΠ½ΠΈΠ΅ΠΉ, ΡΡΠΎ Π½Π΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΎ Π΄Π»Ρ Π΄ΡΡΠ³ΠΈΡ
ΠΠΠ Ρ Π½Π΅ΠΊΡΡΡΡΠΈΡ
Π±ΠΎΠ»ΡΠ½ΡΡ
. Π’Π°ΠΊΠΆΠ΅ ΠΠΠΠ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° ΠΏΡΠΈ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΠΠ Ρ Π΄ΡΡΠ³ΠΈΠΌΠΈ, Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ, Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡΠΌΠΈ
Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction
Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3Γ106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and Ξ±-smooth muscle actin (Ξ±-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of Ξ±-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. Β© Human stem cells institute, 2013
Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction
Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3Γ106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and Ξ±-smooth muscle actin (Ξ±-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of Ξ±-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. Β© Human stem cells institute, 2013
Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction
Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3Γ106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and Ξ±-smooth muscle actin (Ξ±-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of Ξ±-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. Β© Human stem cells institute, 2013
Generic definitional model of linguistic term in scientific learning discourse
Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠ΅Π΄Π»Π°Π³Π°ΡΡΡΡ 4 ΠΌΠΎΠ΄Π΅Π»ΠΈ ΠΏΠΎΡΡΡΠΎΠ΅Π½ΠΈΡ ΡΠΎΠ΄ΠΎΠ²ΠΈΠ΄ΠΎΠ²ΠΎΠΉ Π΄Π΅ΡΠΈΠ½ΠΈΡΠΈΠΈ Π»ΠΈΠ½Π³Π²ΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ΅ΡΠΌΠΈΠ½Π°: 1) ΡΠΎΠ΄ΠΎΠ²ΠΎΠ΅ ΠΏΠΎΠ½ΡΡΠΈΠ΅ ΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°Π΅ΡΡΡ ΠΏΠΎ Π·Π½Π°ΡΠ΅Π½ΠΈΡ ΠΌΠΎΡΡΠ΅ΠΌΡ; 2) ΡΠΎΠ΄ΠΎΠ²ΠΎΠ΅ ΠΏΠΎΠ½ΡΡΠΈΠ΅ ΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°Π΅ΡΡΡ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π°Π½Π°Π»ΠΈΠ·Π° ΡΠΎΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° ΠΏΠΎ ΠΠ‘; Π² 3 ΠΈ 4 ΠΌΠΎΠ΄Π΅Π»ΡΡ
ΡΡΠ½ΠΊΡΠΈΡ ΡΠΎΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠ½ΡΡΠΈΡ Π²ΡΠΏΠΎΠ»Π½ΡΡΡ Π±Π°Π·ΠΎΠ²Π°Ρ Π»ΠΈΠ½Π³Π²ΠΈΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΊΠ°ΡΠ΅Π³ΠΎΡΠΈΡ ΠΈ ΡΠ½ΠΈΠ²Π΅ΡΡΠ°Π»ΡΠ½Π°Ρ ΠΏΠΎΠ½ΡΡΠΈΠΉΠ½Π°Ρ . In the research we suggest and observe four generic definitional models of a linguistic term: 1) the generic notion is set in accordance with the meaning of the morpheme; 2) the generic notion is established by the Immediate Constituent analysis of a generic fragment; in the 3 and 4 models a basic linguistic category and a universal category serve as generic notions
Comparison of different methods of rat hepatic stellate cells isolation, labeling and transplantation
Nowadays more and more attention is turned to hepatic stellate cells (HSC) and their role in liver regeneration. Nonetheless there are several methodological questions, for example, methods of HSC isolation, their labeling and ways of transplantation. In this work we compared two different methods of HSC isolation: collagenase-pronase liver perfusion with further Histodenz density gradient centrifugation and method of Seglen for isolation of hepatocytes associated with HSC. We also analyzed diverse methods of cells labeling: membrane fluorescent labels PKH26 and with gene of green fluorescent protein (GFP), that could be get into the cells by electroporation, with chemicals like TurboFect or by adenovirus. Then cells were transplanted into rats in two ways: into lien and into system of portal vein. According to our results, we able to conclude that collagenase-pronase liver perfusion with further cells gradient centrifugation in Histodenz is better for HSC isolation than method of Seglen, the most optimal method for cells labeling is with adenovirus, expressing the GFP gene, for HSC transplantation - Transplantation into system of portal vein. Β© Human stem cells institute, 2013