Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction

Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3×106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and α-smooth muscle actin (α-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of α-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. © Human stem cells institute, 2013

Comparison of different methods of rat hepatic stellate cells isolation, labeling and transplantation

Nowadays more and more attention is turned to hepatic stellate cells (HSC) and their role in liver regeneration. Nonetheless there are several methodological questions, for example, methods of HSC isolation, their labeling and ways of transplantation. In this work we compared two different methods of HSC isolation: collagenase-pronase liver perfusion with further Histodenz density gradient centrifugation and method of Seglen for isolation of hepatocytes associated with HSC. We also analyzed diverse methods of cells labeling: membrane fluorescent labels PKH26 and with gene of green fluorescent protein (GFP), that could be get into the cells by electroporation, with chemicals like TurboFect or by adenovirus. Then cells were transplanted into rats in two ways: into lien and into system of portal vein. According to our results, we able to conclude that collagenase-pronase liver perfusion with further cells gradient centrifugation in Histodenz is better for HSC isolation than method of Seglen, the most optimal method for cells labeling is with adenovirus, expressing the GFP gene, for HSC transplantation - Transplantation into system of portal vein. © Human stem cells institute, 2013

Влияние рентгенологических признаков коллатеральной вентиляции в сочетании с другими факторами на результаты клапанной бронхоблокации у пациентов с деструктивными формами туберкулеза легких

Introduction. According to studies on the treatment of emphysema, the absence of CT signs of collateral ventilation correlates with the results of valvular blocking procedure, but there is not any data on the relationship between the signs of collateral ventilation and the results of bronchial valve treatment in destructive forms of pulmonary tuberculosis.Study goals: to study the effect of CT features of collateral ventilation in combination with other factors on the results of valvular bronchial blocking in patients with destructivepulmonary pathology in tuberculosis.Materials and methods. Тhe study is based on the results of evaluation of chest CT data of patients with various pathology of pulmonary tuberculosis before and after valvular blocking procedure.Results. Сorrelations between such characteristics as collateral ventilation, additional factors and valvular treatment effects have been determined, and prognostic models estimating the success of valve therapy have been constructed.Conclusion. Positive pleural integrity symptom is a predictor of lobe volume reduction after bronchial valve treatment. Lobe volume reduction during valve therapy is a prognostic factor for cavity’s closure. Введение. По данным исследований, посвященных лечению эмфиземы, отсутствие рентгенологических признаков коллатеральной вентиляции коррелирует с результатами клапанной бронхоблокации, но нет никаких данных о связи между признаками коллатеральной вентиляции и результатами бронхоблокации при деструктивных формах туберкулеза легких.Цель исследования: изучение влияния рентгенологических признаков коллатеральной вентиляции в сочетании с другими факторами на результаты клапанной бронхоблокации у пациентов с деструктивными формами туберкулеза легких.Материалы и методы. Исследование основано на результатах оценки рентгенологом данных компьютерной томографии органов грудной полости пациентов с деструктивными формами туберкулеза легких до и после проведения клапанной бронхоблокации.Результаты. Оценены взаимосвязи между признаками коллатеральной вентиляции, дополнительными факторами и эффектами бронхоблокации, построены прогностические модели достижения результатов клапанной бронхоблокации.Заключение. Положительный симптом целостности плевры является предиктором уменьшения объема доли после клапанной бронхоблокации. Уменьшение объема доли при клапанной бронхоблокации является прогностическим фактором закрытия полости деструкции.

Метод зондовой конфокальной лазерной эндомикроскопии в диагностике идиопатических интерстициальных пневмоний

The diagnosis of idiopathic interstitial pneumonia (IIP) remains one of the most challenging issues in pulmonology. The article demonstrates the capabilities of the method of probe-based confocal laser endomicroscopy (pCLE) in diagnosing various forms of IIPs and provides comparisons with CT and the histological picture. According to the data obtained, it is difficult to distinguish most IIPs from each other using pCLE. Among the specific signs, a large number of alveolar cells in pCLE in a nonsmoking patient with desquamative interstitial pneumonia is not typical for other IIP in nonsmokers. Also, pCLE can be used to differentiate between IIP and other conditions, in particular, malignant lesions.Диагностика идиопатических интерстициальных пневмоний (ИИП) остается одним из сложных вопросов пульмонологии. В статье продемонстрированы возможности метода конфокальной лазерной эндомикроскопии (КЛЭМ) в диагностике различных форм ИИП, приведены сопоставления с данными компьютерной томографии и гистологической картиной. Полученные данные свидетельствуют о том, что большинство ИИП сложно различить между собой при помощи КЛЭМ. Из специфических признаков можно отметить наличие большого количества альвеолярных клеток при КЛЭМ у некурящей пациентки с десквамативной интерстициальной пневмонией, что не характерно для других ИИП у некурящих больных. Также КЛЭМ может быть использована при дифференциальной диагностике ИИП с другими, в частности, злокачественными заболеваниями

Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction

Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3×106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and α-smooth muscle actin (α-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of α-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. © Human stem cells institute, 2013

Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction

Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3×106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and α-smooth muscle actin (α-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of α-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. © Human stem cells institute, 2013

Influence of the human umbilical cord blood mononuclear cells transplantation on regeneration of the rat kidney after unilateral ureteral obstruction

Stem cell therapy may provide effective and patogenetically proved treatment of chronic kidney disease caused by interstitial fibrosis after tubule damage. It was reported that transplanted bone marrow stem cells participate in tubule regeneration [1]. At the same time the potential of hematopoietic stem cells in tubule regeneration is not well investigated. The purpose of the study was to evaluate the participation of human cord blood stem cells in rat kidney tubule regeneration after unilateral ureteral obstruction. 18 laboratory rats were subjected to left unilateral ureteral obstruction (UUO). 3×106 of human cord blood mononuclears (n = 9) or equivalent volume of saline (n = 9) were injected into rat's tail vein. Kidney tissue was collected at the end of the 3, 6 or 14 day after operation. Paraffin-embedded slices were stained with mononuclear antibodies against c-kit (stem and progenitor cell marker), proliferating cell nuclear antigen for the cells proliferative capacity evaluation (PCNA) and α-smooth muscle actin (α-SMA) for myofibroblasts detection. Number of proliferating cells in tubuli ad interstitium was considerably larger in the obstructed kidney of the transplantation group, as well as the number of proliferating cells in the glomeruli at 14 day after operation. At the same time number of α-SMA-positive cells in the transplanted group was significantly lower compared with sham-transplanted group. There were no differences in expression of these markers in the contralateral kidneys. UUO had no impact on c-kit expression in kidney tissue. Thus, transplantation of human cord blood mononuclear cells in UUO stimulates proliferative activity of tubular cells and interstitium, reduces myofibroblast activation and risk of kidney fibrosis. © Human stem cells institute, 2013

Generic definitional model of linguistic term in scientific learning discourse

В статье предлагаются 4 модели построения родовидовой дефиниции лингвистического термина: 1) родовое понятие устанавливается по значению морфемы; 2) родовое понятие устанавливается в результате анализа родового фрагмента по НС; в 3 и 4 моделях функцию родового понятия выполняют базовая лингвистическая категория и универсальная понятийная . In the research we suggest and observe four generic definitional models of a linguistic term: 1) the generic notion is set in accordance with the meaning of the morpheme; 2) the generic notion is established by the Immediate Constituent analysis of a generic fragment; in the 3 and 4 models a basic linguistic category and a universal category serve as generic notions

Comparison of different methods of rat hepatic stellate cells isolation, labeling and transplantation

Nowadays more and more attention is turned to hepatic stellate cells (HSC) and their role in liver regeneration. Nonetheless there are several methodological questions, for example, methods of HSC isolation, their labeling and ways of transplantation. In this work we compared two different methods of HSC isolation: collagenase-pronase liver perfusion with further Histodenz density gradient centrifugation and method of Seglen for isolation of hepatocytes associated with HSC. We also analyzed diverse methods of cells labeling: membrane fluorescent labels PKH26 and with gene of green fluorescent protein (GFP), that could be get into the cells by electroporation, with chemicals like TurboFect or by adenovirus. Then cells were transplanted into rats in two ways: into lien and into system of portal vein. According to our results, we able to conclude that collagenase-pronase liver perfusion with further cells gradient centrifugation in Histodenz is better for HSC isolation than method of Seglen, the most optimal method for cells labeling is with adenovirus, expressing the GFP gene, for HSC transplantation - Transplantation into system of portal vein. © Human stem cells institute, 2013