T lymphocytes derived from human cord blood provide effective antitumor immunotherapy against a human tumor

Abstract Background Although the graft-versus-tumor (GVT) effect of donor-derived T cells after allogeneic hematopoietic stem cell transplantation has been used as an effective adoptive immunotherapy, the antitumor effects of cord blood (CB) transplantation have not been well studied. Methods We established the animal model by transplantation of CB mononuclear cells and/or tumor cells into NOD/SCID mice. The presence of CB derived T cells in NOD/SCID mice or tumor tissues were determined by flow cytometric and immunohistochemical analysis. The anti-tumor effects of CB derived T cells against tumor was determined by tumor size and weight, and by the cytotoxicity assay and ELISPOT assay of T cells. Results We found dramatic tumor remission following transfer of CB mononuclear cells into NOD/SCID mice with human cervical tumors with a high infiltration of CD3+ T cells in tumors. NOD/SCID mice that receive neonatal CB transplants have reconstituted T cells with significant antitumor effects against human cervical and lung tumors, with a high infiltration of CD3+ T cells showing dramatic induction of apoptotic cell death. We also confirmed that T cells showed tumor specific antigen cytotoxicity in vitro. In adoptive transfer of CD3+ T cells into mice with pre-established tumors, we observed much higher antitumor effects of HPV-specific T cells by ELISPOT assays. Conclusions Our results show that CB derived T lymphocytes will be useful for novel immunotherapeutic candidate cells for therapy of several tumors in clinic.</p

A META-ANALYSIS OF TEAMWORK PROCESSES: TESTS OF A MULTIDIMENSIONAL MODEL AND RELATIONSHIPS WITH TEAM EFFECTIVENESS CRITERIA

Drawing from Marks, Mathieu, and Zaccaro (2001), we proposed that narrowly focused teamwork processes load onto 3 higher-order team-work process dimensions, which in turn load onto a general teamwork process factor. Results of model testing using meta-analyses of rela-tionships among narrow teamwork processes provided support for the structure of this multidimensional theory of teamwork process. Meta-analytic results also indicated that teamwork processes have positive relationships with team performance and member satisfaction, and that the relationships are similar across the teamwork dimensions and lev-els of process specificity. Supplemental analyses revealed that the 3 intermediate-level teamwork processes are positively and strongly re-lated to cohesion and potency. Results of moderator analyses suggested that relationships among teamwork processes and team performance are somewhat dependent on task interdependence and team size. Scholars have conducted a great deal of research aimed at understand-ing factors that explain how and why teams achieve desired outcomes (Turner, 2001). The popularity of this type of research is consistent with the reliance on team-based work structures in organizations (Devine, Clayton, Findings reported in this study were presented in a symposium at the 20th Annua

The contribution of creativity of action to safety: the key role of requisite imagination

International audienceThe aim of this paper is to show that requisite imagination by designers and more broadly by all workers – the ability to wonder and imagine key aspects of the future we are planning/designing – can be considered as a manifestation of creative action in organization and should be encouraged. We conducted an intervention in a nuclear power plant facing with a new nationwide policy seeking to extend the lifespan of nuclear facilities through important alterations. The first alteration made turned out badly, due to poor anticipation and a focus on technical effects, whilst organizational aspects were largely excluded. We decided to facilitate the conduct of a collective inquiry with the stakeholders involved with these ongoing evolutions, to discuss organizational design and stimulate requisite imagination. We discuss our main finding from three perspectives: theoretical, methodological and practical

Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes.

Item does not contain fulltextThe BIOMED-2 multiplex polymerase chain reaction (PCR) tubes for analysis of immunoglobulin and T-cell receptor (TCR) gene rearrangements have recently been introduced as a reliable and easy tool for clonality diagnostics in suspected lymphoproliferations. Quality and performance assessment of PCR-based clonality diagnostics is generally performed using human leukemia/lymphoma cell lines as controls. We evaluated the utility of 30 well-defined human T-cell lines for quality performance testing of the BIOMED-2 PCR primers and protocols. The PCR analyses of the TCR loci were backed up by Southern blot analysis. The clonal TCRB, TCRG and TCRD gene rearrangements were analyzed for gene segment usage and for the size and composition of their junctional regions. In 29 out of 30 cell lines, unique clonal TCR gene rearrangements could be easily detected. Besides their usefulness in molecular clonality diagnostics, these cell lines can now be authenticated based on their TCR gene rearrangement profile. This enables their correct use in molecular clonality diagnostics and in other cancer research studies