Intraspecies Transmission of BASE Induces Clinical Dullness and Amyotrophic Changes

The disease phenotype of bovine spongiform encephalopathy (BSE) and the molecular/ biological properties of its prion strain, including the host range and the characteristics of BSE-related disorders, have been extensively studied since its discovery in 1986. In recent years, systematic testing of the brains of cattle coming to slaughter resulted in the identification of at least two atypical forms of BSE. These emerging disorders are characterized by novel conformers of the bovine pathological prion protein (PrPTSE), named high-type (BSE-H) and low-type (BSE-L). We recently reported two Italian atypical cases with a PrPTSE type identical to BSE-L, pathologically characterized by PrP amyloid plaques and known as bovine amyloidotic spongiform encephalopathy (BASE). Several lines of evidence suggest that BASE is highly virulent and easily transmissible to a wide host range. Experimental transmission to transgenic mice overexpressing bovine PrP (Tgbov XV) suggested that BASE is caused by a prion strain distinct from the BSE isolate. In the present study, we experimentally infected Friesian and Alpine brown cattle with Italian BSE and BASE isolates via the intracerebral route. BASE-infected cattle developed amyotrophic changes accompanied by mental dullness. The molecular and neuropathological profiles, including PrP deposition pattern, closely matched those observed in the original cases. This study provides clear evidence of BASE as a distinct prion isolate and discloses a novel disease phenotype in cattle

Loudness differences for Voice-over-Voice audio in TV and streaming

Voice-over-Voice (VoV) is a common mixing practice observed in news reports and docu- mentaries, where a foreground voice is mixed on top of a background voice, e.g., to translate an interview. This is achieved by ducking the background voice so that the foreground voice is more intelligible, while still allowing the listener to perceive the presence and tone of the background voice. Currently there is little published research on ducking practices for VoV or on technical details such as the Loudness Difference (LD) between foreground and background speech. This paper investigates the ducking practices of nine expert audio engineers and the preferred LDs of 13 non-expert listeners of ages 57 years and older. Results highlight a clear difference between the LDs used by the experts and those preferred by the non-expert listeners. Experts tended toward LDs of 11.5–17 LU, while non-experts preferred a range of 20–30 LU. Based on these results, a minimum LD of 20 LU is recommended for VoV. High inter-subject variance due to personal preference was observed. This variance makes a substantial case for the introduction of personalization in broadcast and streaming. The audiovisual material used for the tests is provided at https://www.audiolabs-erlangen.de/resources/2020-VoV-DB