Increasing the Operation Efficiency of Air Conditioning System for Integrated Power Plant on the Base of Its Monitoring

Increasing the Operation Efficiency of Air Conditioning System for Integrated Power Plant on the Base of Its Monitoring / E. Trushliakov, A. Radchenko, S. Forduy, A. Zubarev, A. Hrych // Advances in intelligent systems and computing. – 2020. – Т. 1113 AISC . – P. 351–360Abstract. The efficiency of reciprocating gas engines of integrated energy systems (IES) for combined electricity, heat and refrigeration generation is strictly influenced by their cyclic air temperatures. To evaluate the effect of gas engine cyclic air deep cooling, compared with conventional its cooling, the data on dependence of fuel consumption and power output of gas engine JMS 420 GS-N.L on its inlet air temperature at varying ambient air temperatures at the entrance of the radiator for scavenge air cooling were received. The results of treatment of gas engine efficiency monitoring proved non-effective operation of conventional chilling all the ambient air, coming into the engine room, because of increased air temperature at the inlet of turbocharger (TC), caused by heat influx from surroundings in the engine room. A new method of gas engine inlet air two-stage cooling at increased ambient air temperatures and advanced cyclic air cooling system with absorption lithium-bromide chiller and refrigerant ejector chiller was proposed. With this chilled water from absorption lithiumbromide chiller is used as a coolant in the first high-temperature stage of engine inlet air cooler and boiling refrigerant of ejector chiller in the second lowtemperature stage

3, 3′5 Triiodo L Thyronine Induces Apoptosis in Human Breast Cancer MCF-7cells, Repressing SMP30 Expression through Negative Thyroid Response Elements

Thyroid hormones regulate cell proliferation, differentiation as well as apoptosis. However molecular mechanism underlying apoptosis as a result of thyroid hormone signaling is poorly understood. The antiapoptotic role of Senescence Marker Protein-30 (SMP30) has been characterized in response to varieties of stimuli as well as in knock out model. Our earlier data suggest that thyroid hormone 3, 3'5 Triiodo L Thyronine (T(3)), represses SMP30 in rat liver.In highly metastatic MCF-7, human breast cancer cell line T3 treatment repressed SMP30 expression leading to enhanced apoptosis. Analysis by flow cytometry and other techniques revealed that overexpression and silencing of SMP30 in MCF-7 resulted in decelerated and accelerated apoptosis respectively. In order to identify the cis-acting elements involved in this regulation, we have analyzed hormone responsiveness of transiently transfected hSMP30 promoter deletion reporter vectors in MCF-7 cells. As opposed to the expected epigenetic outcome, thyroid hormone down regulated hSMP30 promoter activity despite enhanced recruitment of acetylated H3 on thyroid response elements (TREs). From the stand point of established epigenetic concept we have categorised these two TREs as negative response elements. Our attempt of siRNA mediated silencing of TRβ, reduced the fold of repression of SMP30 gene expression. In presence of thyroid hormone, Trichostatin- A (TSA), which is a Histone deacetylase (HDAC) inhibitor further inhibited SMP30 promoter activity. The above findings are in support of categorisation of both the thyroid response element as negative response elements as usually TSA should have reversed the repressions.This is the first report of novel mechanistic insights into the remarkable downregulation of SMP30 gene expression by thyroid hormone which in turn induces apoptosis in MCF-7 human breast cancer cells. We believe that our study represents a good ground for future effort to develop new therapeutic approaches to challenge the progression of breast cancer