Betalains and phenolic compounds of leaves and stems of Alternanthera brasiliana and Alternanthera tenella

Betacyanins and phenolic compounds from acetonitrile:acidified water extracts of Alternanthera brasiliana and Alternanthera tenella were characterized and quantified using a high-performance liquid chromatography system coupled with diode array and electrospray mass spectrometry detection. Four betacyanins (amaranthine, isoamaranthine, betanin and isobetanin) were tentatively identified and quantified. Twenty eight phenolic compounds of four different families (hydroxybenzoic and hydroxycinnamic acids, flavones and flavonols) were separated and characterized on the basis of their accurate MS and MS/MS information out of which ten compounds were confirmed by authentic standards. These plant species could be considered as an especially rich source of natural bioactive compounds and potential food colorants. A. brasiliana showed the highest betacyanin and polyphenols content (89 μg/g and 35,243 μg/g, respectively). Among polyphenols, flavonols were the more abundant (kaempferol-glucoside, kaempferol-rutinoside and kaempferol-rhamnosyl-rhamnosyl-glycoside). Meanwhile, A. tenella showed a different polyphenols profile with flavones as major compounds (glucopyranosil-vitexin and vitexin). As a novelty, pentosyl-vitexin and pentosyl-isovitexin were detected for the first time in Alternanthera plants. Both A. brasiliana and A. tenella leaves showed high total polyphenol content and in vitro antioxidant activity (FRAP). These results provide an analytical base concerning the phenolic and betalains composition and the antioxidant properties of two members of the promising Alternanthera gender, for subsequent applications, such as functional food ingredients.Fil: Deladino, Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Alvarez, I.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; EspañaFil: De Ancos, B.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; EspañaFil: Sánchez Moreno, C.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; EspañaFil: Molina García, A. D.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; EspañaFil: Schneider Teixeira, Aline. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; Españ

Spikes in Cosmic Crystallography

If the universe is multiply connected and small the sky shows multiple images of cosmic objects, correlated by the covering group of the 3-manifold used to model it. These correlations were originally thought to manifest as spikes in pair separation histograms (PSH) built from suitable catalogues. Using probability theory we derive an expression for the expected pair separation histogram (EPSH) in a rather general topological-geometrical-observational setting. As a major consequence we show that the spikes of topological origin in PSH's are due to translations, whereas other isometries manifest as tiny deformations of the PSH corresponding to the simply connected case. This result holds for all Robertson-Walker spacetimes and gives rise to two basic corollaries: (i) that PSH's of Euclidean manifolds that have the same translations in their covering groups exhibit identical spike spectra of topological origin, making clear that even if the universe is flat the topological spikes alone are not sufficient for determining its topology; and (ii) that PSH's of hyperbolic 3-manifolds exhibit no spikes of topological origin. These corollaries ensure that cosmic crystallography, as originally formulated, is not a conclusive method for unveiling the shape of the universe. We also present a method that reduces the statistical fluctuations in PSH's built from simulated catalogues.Comment: 25 pages, LaTeX2e. References updated. To appear in Int. J. Mod. Phys. D (2002) in the present for

Derivation of an Abelian effective model for instanton chains in 3D Yang-Mills theory

In this work, we derive a recently proposed Abelian model to describe the interaction of correlated monopoles, center vortices, and dual fields in three dimensional SU(2) Yang-Mills theory. Following recent polymer techniques, special care is taken to obtain the end-to-end probability for a single interacting center vortex, which constitutes a key ingredient to represent the ensemble integration.Comment: 18 pages, LaTe

Topological Reverberations in Flat Space-times

We study the role played by multiply-connectedness in the time evolution of the energy E(t) of a radiating system that lies in static flat space-time manifolds M_4 whose t=const spacelike sections M_3 are compact in at least one spatial direction. The radiation reaction equation of the radiating source is derived for the case where M_3 has any non-trivial flat topology, and an exact solution is obtained. We also show that when the spacelike sections are multiply-connected flat 3-manifolds the energy E(t) exhibits a reverberation pattern with discontinuities in the derivative of E(t) and a set of relative minima and maxima, followed by a growth of E(t). It emerges from this result that the compactness in at least one spatial direction of Minkowski space-time is sufficient to induce this type of topological reverberation, making clear that our radiating system is topologically fragile. An explicit solution of the radiation reaction equation for the case where M_3 = R^2 x S^1 is discussed, and graphs which reveal how the energy varies with the time are presented and analyzed.Comment: 16 pages, 4 figures, REVTEX; Added five references and inserted clarifying details. Version to appear in Int. J. Mod. Phys. A (2000

Microfluidic encapsulation method to produce stable liposomes containing iohexol

Since the discovery of X-rays in the late 1890s, several medical imaging techniques have been developed, such as Computed Tomography (CT), Magnetic Resonance Imaging (MRI) and Ultrasound Imaging, which are used daily to diagnose, monitor, or treat medical conditions. Some of these techniques include the use of contrast agents to enhance the contrast images, therefore, toxic effects must be considered. Among these, Contrast-Induced Nephropathy (CIN) is an acute renal failure resulting from the administration of iodinated contrast media (CM). To date, there is no definitive treatment for CIN and several prevention approaches have been evaluated. Nanoparticles (NPs) represent a promising strategy for treatment and prevention of CIN, due to their ability to deliver CM during diagnosis imaging. In this study, iohexol-containing liposomes were produced using microfluidic technique for first time. Several phosphocholine lipids (e.g. DMPC, DOPC, DPPC and DSPC) with cholesterol (2:1 ratio) were investigated and DLS, FTIR and in vitro release studies at 37 °C were performed, with stability studies conducted on the best formulation. The microfluidic method allowed to obtain a high encapsulation efficiency (over 70%), and release profiles showed an iohexol release around or less than 0.12 mg/ml after 2 h for the majority of the formulations, which is not toxic to the kidney cells