Identification and Characterisation of Pseudomonas 16S Ribosomal DNA from Ileal Biopsies of Children with Crohn's Disease

Molecular analysis of bacterial 16S rRNA genes has made a significant contribution to the identification and characterisation of bacterial flora in the human gut. In particular, this methodology has helped characterise bacterial families implicated in the aetiology of inflammatory bowel disease (IBD). In this study we have used a genus specific bacterial 16S PCR to investigate the prevalence and diversity of Pseudomonas species derived from the ileum of children with Crohn's disease (CD), and from control children with non-inflammatory bowel disease (non-IBD) undergoing their initial endoscopic examination. Fifty eight percent of CD patients (18/32) were positive using the Pseudomonas PCR, while significantly fewer children in the non-IBD group, 33% (12/36), were PCR positive for Pseudomonas (p<0.05, Fischer's exact test). Pseudomonas specific 16S PCR products from 13 CD and 12 non-IBD children were cloned and sequenced. Five hundred and eighty one sequences were generated and used for the comparative analysis of Pseudomonas diversity between CD and non-IBD patients. Pseudomonas species were less diverse in CD patients compared with non-IBD patients. In particular P.aeruginosa was only identified in non-IBD patients

An intense stratospheric jet on Jupiter.

The Earth's equatorial stratosphere shows oscillations in which the east-west winds reverse direction and the temperatures change cyclically with a period of about two years. This phenomenon, called the quasi-biennial oscillation, also affects the dynamics of the mid- and high-latitude stratosphere and weather in the lower atmosphere. Ground-based observations have suggested that similar temperature oscillations (with a 4-5-yr cycle) occur on Jupiter, but these data suffer from poor vertical resolution and Jupiter's stratospheric wind velocities have not yet been determined. Here we report maps of temperatures and winds with high spatial resolution, obtained from spacecraft measurements of infrared spectra of Jupiter's stratosphere. We find an intense, high-altitude equatorial jet with a speed of approximately 140 m s(-1), whose spatial structure resembles that of a quasi-quadrennial oscillation. Wave activity in the stratosphere also appears analogous to that occurring on Earth. A strong interaction between Jupiter and its plasma environment produces hot spots in its upper atmosphere and stratosphere near its poles, and the temperature maps define the penetration of the hot spots into the stratosphere

Jupiter's atmospheric composition from the Cassini thermal infrared spectroscopy experiment.

The Composite Infrared Spectrometer observed Jupiter in the thermal infrared during the swing-by of the Cassini spacecraft. Results include the detection of two new stratospheric species, the methyl radical and diacetylene, gaseous species present in the north and south auroral infrared hot spots; determination of the variations with latitude of acetylene and ethane, the latter a tracer of atmospheric motion; observations of unexpected spatial distributions of carbon dioxide and hydrogen cyanide, both considered to be products of comet Shoemaker-Levy 9 impacts; characterization of the morphology of the auroral infrared hot spot acetylene emission; and a new evaluation of the energetics of the northern auroral infrared hot spot

Temperatures, winds, and composition in the saturnian system.

Stratospheric temperatures on Saturn imply a strong decay of the equatorial winds with altitude. If the decrease in winds reported from recent Hubble Space Telescope images is not a temporal change, then the features tracked must have been at least 130 kilometers higher than in earlier studies. Saturn's south polar stratosphere is warmer than predicted from simple radiative models. The C/H ratio on Saturn is seven times solar, twice Jupiter's. Saturn's ring temperatures have radial variations down to the smallest scale resolved (100 kilometers). Diurnal surface temperature variations on Phoebe suggest a more porous regolith than on the jovian satellites

Titan's atmospheric temperatures, winds, and composition.

Temperatures obtained from early Cassini infrared observations of Titan show a stratopause at an altitude of 310 kilometers (and 186 kelvin at 15 degrees S). Stratospheric temperatures are coldest in the winter northern hemisphere, with zonal winds reaching 160 meters per second. The concentrations of several stratospheric organic compounds are enhanced at mid- and high northern latitudes, and the strong zonal winds may inhibit mixing between these latitudes and the rest of Titan. Above the south pole, temperatures in the stratosphere are 4 to 5 kelvin cooler than at the equator. The stratospheric mole fractions of methane and carbon monoxide are (1.6 +/- 0.5) x 10(-2) and (4.5 +/- 1.5) x 10(-5), respectively

Exploring the Saturn system in the thermal infrared: The Composite Infrared Spectrometer

The Composite Infrared Spectrometer (CIRS) is a remote-sensing Fourier Transform Spectrometer (FTS) on the Cassini orbiter that measures thermal radiation over two decades in wavenumber, from 10 to 1400 cm- 1 (1 mm to 7μ m), with a spectral resolution that can be set from 0.5 to 15.5 cm- 1. The far infrared portion of the spectrum (10-600 cm - 1) is measured with a polarizing interferometer having thermopile detectors with a common 4-mrad field of view (FOV). The middle infrared portion is measured with a traditional Michelson interferometer having two focal planes (600-1100 cm- 1, 1100-1400 cm- 1). Each focal plane is composed of a 1× 10 array of HgCdTe detectors, each detector having a 0.3-mrad FOV. CIRS observations will provide three-dimensional maps of temperature, gas composition, and aerosols/condensates of the atmospheres of Titan and Saturn with good vertical and horizontal resolution, from deep in their tropospheres to high in their mesospheres. CIRS's ability to observe atmospheres in the limb-viewing mode (in addition to nadir) offers the opportunity to provide accurate and highly resolved vertical profiles of these atmospheric variables. The ability to observe with high-spectral resolution should facilitate the identification of new constituents. CIRS will also map the thermal and compositional properties of the surfaces of Saturn's icy satellites. It will similarly map Saturn's rings, characterizing their dynamical and spatial structure and constraining theories of their formation and evolution. The combination of broad spectral range, programmable spectral resolution, the small detector fields of view, and an orbiting spacecraft platform will allow CIRS to observe the Saturnian system in the thermal infrared at a level of detail not previously achieved. © 2004 Kluwer Academic Publishers

A massive core for a cluster of galaxies at a redshift of 4.3

Massive galaxy clusters are now found as early as 3 billion years after the Big Bang, containing stars that formed at even earlier epochs. The high-redshift progenitors of these galaxy clusters, termed 'protoclusters', are identified in cosmological simulations with the highest dark matter overdensities. While their observational signatures are less well defined compared to virialized clusters with a substantial hot intra-cluster medium (ICM), protoclusters are expected to contain extremely massive galaxies that can be observed as luminous starbursts. Recent claimed detections of protoclusters hosting such starbursts do not support the kind of rapid cluster core formation expected in simulations because these structures contain only a handful of starbursting galaxies spread throughout a broad structure, with poor evidence for eventual collapse into a protocluster. Here we report that the source SPT2349-56 consists of at least 14 gas-rich galaxies all lying at z = 4.31 based on sensitive observations of carbon monoxide and ionized carbon. We demonstrate that each of these galaxies is forming stars between 50 and 1000 times faster than our own Milky Way, and all are located within a projected region only $\sim$ 130 kiloparsecs in diameter. This galaxy surface density is more than 10 times the average blank field value (integrated over all redshifts) and $>$1000 times the average field volume density. The velocity dispersion ($\sim$ 410 km s$^{-1}$) of these galaxies and enormous gas and star formation densities suggest that this system represents a galaxy cluster core at an advanced stage of formation when the Universe was only 1.4 billion years old. A comparison with other known protoclusters at high redshifts shows that SPT2349-56 is a uniquely massive and dense system that could be building one of the most massive structures in the Universe today.Comment: To appear in April 26 issue of Natur