33 research outputs found

    Control of DNA synthesis genes in budding yeast: involvement of the transcriptional modulator MOT1 in the expression of the DNA polymerase alpha gene

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    Periodic transcription during the cell cycle of the budding yeast DNA polymerase \u3b1 gene (POLI) requires the cis-acting element 5\u2032 ACGCGT 3\u2032, which has been found in the 5\u2032 non-coding region of all the DNA synthesis genes analyzed so far. Search for trans-acting mutations affecting POL1 expression led to the isolation of the temperature-sensitive reg1033 mutant, that showed increased levels of both DNA polymerase \u3b1 and \u3b4 gene transcripts. Cloning of the REG1033 gene demonstrated that it is essential for cell viability and required for proper expression of the POL1 gene. DNA sequence comparison established that the REG1033 gene is identical to MOT1, a gene encoding a presumptive DNA helicase which modulates transcription of several yeast genes
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