Two-Color Analysis of Epidermal Cell Suspension of Mouse with Anti-DNP and Anti-Thy-1 Antibodies after Skin Painting with DNCB

The epidermal cell suspensions prepared from DNCB painted ear skin of C3H/He mice were double-stained for Thy-1 protein and DNP groups. The cells with both Thy-1 alloantigen and DNP groups in the specimen treated with anti-DNP and followed by anti-Thy-1,2 incubation were detected more frequently than the cells treated with the antibodies in the reverse order. This suggests that DNCB is coupled in vivo to the Thy-1 alloantigen on the surface of Thy-1 positive cells

The Effect of Tape Stripping on the Fate of Intradermally Injected Dinitrophenylated Isogeneic Epidermal Cells and Allogeneic Epidermal Cells in Guinea Pigs

DNP-iso ECs or alio ECs were injected intradermally into normal or tape stripped ear skin of inbred JY-1 strain guinea pigs. The fate of the injected cells was histologically examined. The cells proliferated in the dermis, formed EC nests with central keratinization and then elicited a reject reaction associated with necrosis of the epidermal structures in both normal and stripped skin. The suppressive effect of the tape stripping treatment on the reaction was not observed. The significance of the findings is discussed

Ultrastructural Localization of DNP Groups on Draining Lymph Node Cells of Guinea Pigs Following Skin Painting with DNCB : II. Immunoferritin Electron Microscopic Study

The distribution of DNP groups on the cells taken from the draining lymph nodes of guinea pigs 12 hours after painting the skin with DNCB was examined by transmission immunoelectron microscopy using the antibody against DNP groups and ferritin as visual marker. The study showed that DNP groups were distributed on the surface of lymphocytes, both T and B cells, and macrophages. It is suggested that DNCB penetrates through skin into lymphatics carrying lymph from the painted site and combines with the membrane of lymphocytes and macrophages in the peripheral lymphoid system and that the resulting modified cells act as immunogens or tolerogens in contact sensitivity

Antigen Specificity of Retest Reaction in Contact Sensitivity

Guinea pigs doubly sensitized to 2,4-dinitrochlorobenzene (DNCB) and oxazolone (OX) were tested with DNCB, OX and croton oil and retested by epicutaneous application of DNCB and OX at the sites of prior contact reaction at the various intervals following skin testing. Antigen specificity of retest reaction to DNCB was demonstrated when retest of DNCB was performed 21 and 28 days after the skin testing. The significance of the findings is discussed