Ultrastructural Localization of DNP Groups on Draining Lymph Node Cells of Guinea Pigs Following Skin Painting with DNCB : II. Immunoferritin Electron Microscopic Study

Abstract

The distribution of DNP groups on the cells taken from the draining lymph nodes of guinea pigs 12 hours after painting the skin with DNCB was examined by transmission immunoelectron microscopy using the antibody against DNP groups and ferritin as visual marker. The study showed that DNP groups were distributed on the surface of lymphocytes, both T and B cells, and macrophages. It is suggested that DNCB penetrates through skin into lymphatics carrying lymph from the painted site and combines with the membrane of lymphocytes and macrophages in the peripheral lymphoid system and that the resulting modified cells act as immunogens or tolerogens in contact sensitivity