ワタシ ノ コウギ スポーツ ニ エイセイ オ モトメテ

私が担当する講義は、衛生･公衆衛生学、環境保健学、スポーツ衛生学などです。｢体育専門学群に、なぜ衛生学や環境保健学が必要なのか?｣この問への答えを求めて講義内容を入れ替え、スポーツの現場の声を聞き、再び自問自答を繰り返してきました。

胸壁外に設置した拍動型補助人工心臓に関する前臨床的研究

An assistance artificial heart was fabricated with blood compatible polyurethane　having 3 different sizes and was evaluated by a series of preclinical experiment in　calves. The following results were obtained. 1) The artificial heart was constructed in 3 sizes of 60, 80 and 100 cc in capacity　and is activated by a pneumatic heart driver console. 2) The mock circulation revealed that this assistance heart has ejection fraction of　80-95%. 3) Safe use of assistance heart has been confirmed by implantation in 18 calves　with intact or failured hearts over a period of 3 months. 4) Weaning could be performed in cases where myocardial necrosis of the left　ventricle is induced less than 35% utilizing myocardial injection of 5N-NaOH. However　pump dependency develops for a necrosis exceeding 35 %. 5) Thus we concluded that this system can be safely employed for a one month　cardiac assistance clinically and at most for a 3 months support in pump dependent　cases. 6) The technique was developed to make the instant shift possible within a few　minutes from the conventional non-pulsatile bypass assistance to the assistance heart

Determination of ¹³⁵Cs and ¹³⁵Cs/¹³⁷Cs Atomic Ratio in Environmental Samples by Combining Ammonium Molybdophosphate (AMP)-Selective Cs Adsorption and Ion-Exchange Chromatographic Separation to Triple-Quadrupole Inductively Coupled Plasma–Mass Spectrometry

Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident in 2011, the activity ratio of ¹³⁴Cs/¹³⁷Cs has been widely used as a tracer for contamination source identification. However, because of the short half-life of ¹³⁴Cs (2.06 y), this tracer will become unavailable in the near future. This article presents an analytical method for the determination of the long-lived ¹³⁵Cs (<i>t</i>_2/1 = 2 × 10⁶ y) and the atomic ratio of ¹³⁵Cs/¹³⁷Cs, as a promising geochemical tracer, in environmental samples. The analytical method involves ammonium molybdophosphate (AMP)-selective adsorption of Cs and subsequent two-stage ion-exchange chromatographic separation, followed by detection of isolated radiocesium isotopes via triple-quadrupole inductively coupled plasma–mass spectrometry (ICP-MS/MS). The AMP-selective adsorption of Cs and the chromatographic separation system showed high decontamination factors (10⁴–10⁵) for interfering elements, such as Ba, Mo, Sb, and Sn. Using ICP-MS/MS, only selected ions enter the collision/reaction cell to react with N₂O, reducing the isobaric interferences (¹³⁵Ba⁺ and ¹³⁷Ba⁺) and polyatomic interferences (⁹⁵ Mo⁴⁰Ar⁺, ⁹⁷ Mo⁴⁰Ar⁺, ¹¹⁹Sn¹⁶O⁺, and ¹²¹Sb¹⁶O⁺) produced by sample matrix ions. The high abundance sensitivity (10^–9 for the ¹³⁵Cs/¹³³Cs ratio) provided by ICP-MS/MS allowed reliable analysis of ¹³⁵Cs and ¹³⁷Cs isotopes with the lowest detection limits ever reported by mass counting methods (0.01 pg mL^–1 and 0.006 pg mL^–1, respectively). The developed analytical method was successfully applied to the determination of ¹³⁵Cs and ¹³⁷Cs isotopes in environmental samples (soil, litter, and lichen) collected after the FDNPP accident for contamination source identification

Transcription Activity of Individual rrn Operons in Bacillus subtilis Mutants Deficient in (p)ppGpp Synthetase Genes, relA, yjbM, and ywaC▿ †

In Bacillus subtilis a null mutation of the relA gene, whose gene product is involved in the synthesis and/or hydrolysis of (p)ppGpp, causes a growth defect that can be suppressed by mutation(s) of yjbM and/or ywaC coding for small (p)ppGpp synthetases. All 35 suppressor mutations newly isolated were classified into two groups, either yjbM or ywaC, by mapping and sequencing their mutations, suggesting that there are no (p)ppGpp synthetases other than RelA, YjbM, and YwaC in B. subtilis. In order to understand better the relation between RelA and rRNA synthesis, we studied in the relA mutant the transcriptional regulation of seven rRNA operons (rrnO, -A, -J, -I, -E, -D, or -B) individually after integration of a promoter- and terminatorless cat gene. We identified the transcriptional start sites of each rrn operon (a G) and found that transcription of all rrn operons from their P1 promoters was drastically reduced in the relA mutant while this was almost completely restored in the relA yjbM ywaC triple mutant. Taken together with previous results showing that the intracellular GTP concentration was reduced in the relA mutant while it was restored in the triple mutant, it seems likely that continuous (p)ppGpp synthesis by YjbM and/or YwaC at a basal level causes a decrease in the amounts of intracellular GTP