Evolution of replication efficiency following infection with a molecularly cloned feline immunodeficiency virus of low virulence

The development of an effective vaccine against human immunodeficiency virus is considered to be the most practicable means of controlling the advancing global AIDS epidemic. Studies with the domestic cat have demonstrated that vaccinal immunity to infection can be induced against feline immunodeficiency virus (FIV); however, protection is largely restricted to laboratory strains of FIV and does not extend to primary strains of the virus. We compared the pathogenicity of two prototypic vaccine challenge strains of FIV derived from molecular clones; the laboratory strain PET<sub>F14</sub> and the primary strain GL8<sub>414</sub>. PET<sub>F14</sub> established a low viral load and had no effect on CD4<sup>+</sup>- or CD8<sup>+</sup>- lymphocyte subsets. In contrast, GL8<sub>414</sub> established a high viral load and induced a significant reduction in the ratio of CD4<sup>+</sup> to CD8<sup>+</sup> lymphocytes by 15 weeks postinfection, suggesting that PET<sub>F14</sub> may be a low-virulence-challenge virus. However, during long-term monitoring of the PET<sub>F14</sub>-infected cats, we observed the emergence of variant viruses in two of three cats. Concomitant with the appearance of the variant viruses, designated 627<sub>W135</sub> and 628<sub>W135</sub>, we observed an expansion of CD8<sup>+</sup>-lymphocyte subpopulations expressing reduced CD8 ß-chain, a phenotype consistent with activation. The variant viruses both carried mutations that reduced the net charge of the V3 loop (K409Q and K409E), giving rise to a reduced ability of the Env proteins to both induce fusion and to establish productive infection in CXCR4-expressing cells. Further, following subsequent challenge of naïve cats with the mutant viruses, the viruses established higher viral loads and induced more marked alterations in CD8<sup>+</sup>-lymphocyte subpopulations than did the parent F14 strain of virus, suggesting that the E409K mutation in the PET<sub>F14</sub> strain contributes to the attenuation of the virus

Densely integrated photonic devices based on microring resonators for use in access networks

A reconfigurable optical add-drop multiplexer as well as a 1x4x4 reconfigurable λ router, designed for use in the second telecom window, are demonstrated. The devices that each have a footprint less than 2 mm$_{2}$ are based on thermally tunable vertically coupled microring resonators fabricated in $Si_{3}N_{4}$/$SiO_{2}$

Quantum dots in magnetic fields: thermal response of broken symmetry phases

We investigate the thermal properties of circular semiconductor quantum dots in high magnetic fields using finite temperature Hartree-Fock techniques. We demonstrate that for a given magnetic field strength quantum dots undergo various shape phase transitions as a function of temperature, and we outline possible observable consequences.Comment: In Press, Phys. Rev. B (2001

Vaccination with an Inactivated Virulent Feline Immunodeficiency Virus Engineered to Express High Levels of Env

An inactivated virus vaccine was prepared from a pathogenic isolate of feline immunodeficiency virus containing a mutation that eliminated an endocytic sorting signal in the envelope glycoprotein, increasing its expression on virions. Cats immunized with inactivated preparations of this modified virus exhibited strong titers of antibody to Env by enzyme-linked immunosorbent assay. Evidence of protection following challenge demonstrated the potential of this approach to lentiviral vaccination

Spin Exciton in quantum dot with spin orbit coupling in high magnetic field

Coulomb interactions of few ($N$) electrons confined in a disk shaped quantum dot, with a large magnetic field $B=B^*$ applied in the z-direction (orthogonal to the dot), produce a fully spin polarized ground state. We numerically study the splitting of the levels corresponding to the multiplet of total spin $S=N/2$ (each labeled by a different total angular momentum $J_z$) in presence of an electric field parallel to $B$, coupled to $S$ by a Rashba term. We find that the first excited state is a spin exciton with a reversed spin at the origin. This is reminiscent of the Quantum Hall Ferromagnet at filling one which has the skyrmion-like state as its first excited state. The spin exciton level can be tuned with the electric field and infrared radiation can provide energy and angular momentum to excite it.Comment: 9 pages, 9 figures. submitted to Phys.Rev.

Kondo Effect of Quantum Dots in the Quantum Hall Regime

Quantum dots in the quantum Hall regime can have pairs of single Slater determinant states that are degenerate in energy. We argue that these pairs of many body states may give rise to a Kondo effect which can be mapped into an ordinary Kondo effect in a fictitious magnetic field. We report on several properties of this Kondo effect using scaling and numerical renormalization group analysis. We suggest an experiment to investigate this Kondo effect.Comment: To appear in Phys. Rev. B (5 pages, 4 figures); references added; several changes in tex

Success or failure in knowledge management systems: a universal issue

This paper takes a sociotechnical viewpoint of knowledge management system (KMS) implementation in organizations considering issues such as stakeholder disenfranchisement, lack of communication, and the low involvement of key personnel in system design asking whether KMS designers could learn from applying sociotechnical principles to their systems. The paper discusses design elements drawn from the sociotechnical principles essential for the success of IS and makes recommendations to increase the success of KMS in organizations. It also provides guidelines derived from Clegg’s Principles (2000) for KMS designers to enhance their designs. Our data comes from the application of a plurality of analysis methods on a large comprehensive global survey conducted from 2007 to 2011 of 1034 participants from 76 countries. The survey covers a variety of organizations of all types and sizes from a comprehensive selection of economic sectors and industries. Our results showed that users were not satisfied with the information and knowledge systems that they were being offered. In addition to multiple technology and usability issues, there were human and organisational barriers that prevented the systems from being used to their full potential. We recommend that users of KMS are integrated into the design team so that these usability and other barriers can be addressed during the feasibility stage as well as the actual design and implementation phases

Strongly correlated quantum dots in weak confinement potentials and magnetic fields

We explore a strongly correlated quantum dot in the presence of a weak confinement potential and a weak magnetic field. Our exact diagonalization studies show that the groundstate property of such a quantum dot is rather sensitive to the magnetic field and the strength of the confinement potential. We have determined rich phase diagrams of these quantum dots. Some experimental consequences of the obtained phase diagrams are discussed.Comment: 5 pages, 7 figures, new and updated figure

The Designers Leap: Boundary Jumping to foster interdisciplinarity between Textile Design and Science

Creative thinking is an instinctive problem-solving process for designers however, designers alone cannot solve real-world problems. Collaboration between higher education and industry, and across design and science disciplines can create new paradigms of research to address societal and economic challenges. This paper argues from the perspective of fashion and textile designers, for design to be at the heart of the collaborative research process and advocates for co-design, speculative-design and scenario design to be considered as valid methodologies to foster interdisciplinarity. Perspectives on interdisciplinary partnerships across academic disciplines and with industry are typified by two very different reflections of collaborative projects between fashion designers, textile designers, scientists and industrial partners. The paper identifies commonalities and differences between scientists and designers, with particular relevance to textiles, in a bid to understand how they may collaborate more effectively in the context of interdisciplinary work, and the paper further identifies factors needed for establishing common enablers for engaging in co-design. This is an under-explored field and highlights the changing role of the designer, and as such is of value to researchers in textiles, fashion and product design

Kinetic Turbulence

The weak collisionality typical of turbulence in many diffuse astrophysical plasmas invalidates an MHD description of the turbulent dynamics, motivating the development of a more comprehensive theory of kinetic turbulence. In particular, a kinetic approach is essential for the investigation of the physical mechanisms responsible for the dissipation of astrophysical turbulence and the resulting heating of the plasma. This chapter reviews the limitations of MHD turbulence theory and explains how kinetic considerations may be incorporated to obtain a kinetic theory for astrophysical plasma turbulence. Key questions about the nature of kinetic turbulence that drive current research efforts are identified. A comprehensive model of the kinetic turbulent cascade is presented, with a detailed discussion of each component of the model and a review of supporting and conflicting theoretical, numerical, and observational evidence.Comment: 31 pages, 3 figures, 99 references, Chapter 6 in A. Lazarian et al. (eds.), Magnetic Fields in Diffuse Media, Astrophysics and Space Science Library 407, Springer-Verlag Berlin Heidelberg (2015