Die Isolierung von ß-4N-Glukosidase aus Kaninchenplasma und die Untersuchung ihrer Eigenschaften mit Sulfonamid-ß-4N-Glukosiden

Six different sulphonamide-ß-4N-glucosides were studied by a hydrolysing enzyme, the ß-4N-glucosidase, prepared from rabbit blood plasma. On purification, the specific activity rises 200-250 times in preparation and 75-80 per cent of originalactivity can be obtained.The hydrolysis is reversible. The velocity of the enzymic reaction is influenced by the different substituents group on the NH2. The reaction has zero-rate in the initial stages of the hydrolysis changing to first-rate and, finally, polymolecular effectsdominate on reaching the equilibrium state. The dependence of enzyme activity on substrate concentration has been examined and the KM's for each sulphonamide ß-4N-glucoside have been calculated. The temperature and and pH Optimum are 40? andpH 7 respectively, The ß-4N-glucosidase can be strongly activated by K+, Na+ and Mg2+ and slightly by Ca2+. The NH4+ ion has a negligible effect but Zn2+ and Cu2+ inhibit. The enzyme is competitively inhibited by sulphonamide molecules liberatedfrom ß-4N-glucoside

Endotoxins do not influence transplacental transmission of lymphotropic human herpesviruses and human papillomaviruses into amniotic fluid taken from healthy mothers before parturition in Hungary

Pregnant women were examined following healthy pregnancies at term. Amniotic fluids were sampled before arteficial rupture of membranes using closed vacutainer system. Blood samples were also taken from the pregnants simultaneously. Endotoxin concentrations of amniotic fluids were tested by the semiquantitative Limulus amebocyte lysate. Both amniotic fluids and blood samples were tested for the presence of DNA of lymphotropic human herpesviruses. The DNA of human papillomaviruses were tested only in the amniotic fluid samples. One-third of the amniotic fluids tested were found to contain measurable amounts of endotoxin. Lymphotropic herpesvirus DNA was deteced in every fourth amniotic fluid sample and in every 8th blood sample. The prevalence of papillomaviruses was 7 of 96 samples. No significant correlation was found between the presence of endotoxin and viruses in the amniotic fluids. Epstein-Barr virus, human cytomegalovirus and human herpesvirus type 7 were found more frequently in the amniotic fluids than in blood samples (7 to 1). The prevalence of human herpesvirus 6 and 8 was higher in the blood samples than that in the amniotic fluids. The mean weight of the neonates were not impaired significantly by the presence of either viruses or endotoxin. Possible post partum consequences, i.e. partial immunotolerance to viruses is discussed