Suitability of various lignocellulosic substrates for cultivation of Pleurotus sajor-caju (Oyster Mushroom)

The objective of this study was to look into the possibility of using the different lignocellulosic biomasses for Pleurotus sajor-caju (oyster mushroom) cultivation. The mushroom species was cultivated on nine types of substrates; namely three acacia species, three types of straws, coffee husk and saw dust obtained from two types of timber species. Thirty types of treatments (pure and mixed) were used to grow the mushroom and significantly differing results were obtained. Yield data (biological efficiency, spawn running period (SRP) and days to first harvest (DFH) of the mushroom were measured. The shortest SPR and DFH were from the pure chopped barley straw (BS) followed by pure wheat straw (WS) and Acacia drepanolobium (DC), respectively. The maximum period was from the mixed sawdust of Aningeria adolfi-friedericii (AS) and Cupressus lusitanica (CS) in the ratio of 4:6. The least mushroom biological efficiency was also from the ASCS4 (4:6) mixture. No any fruit body appeared on the pure C. lustanica sawdust (CS) and the mixture of ASCS in a ratio of 3:7. On the other hand, the maximum biological efficiency of the mushroom was obtained from DC followed by BS, WS and SC, respectively. In conclusion, mushroom biological efficiencies of the pure A. drepanolobium and A. seyal substrates were found to be better than those of the mixed ones. These biological efficiencies were also found to be better than those of the agri-residues.Keywords: Pleuroteus sajor caju, Acacia species, coffee husk, sawdust, straws, bush contro

Determination of Local Barley (Hordeum Vulgare) Crop Coefficient and Comparative Assessment of Water Productivity for Crops Grown Under the Present Pond Water in Tigray, Northern Ethiopia

An experiment was carried out in 2010 at Mekelle, in northern Ethiopia, to measure the evapotranspiration, to estimate barley crop coefficient (kc), and to evaluate the water productivity taking into account the major crops grown under the present pond irrigation system. Four locally made lysimters were installed in the middle of barley field to measure barley evapotranspiration. The single crop coefficient approach was used to estimate barley crop coefficient. The average seasonal evapotranspiration of barley was 375 mm which is similar to many other cereal crops in the region. The single crop coefficient values for early, vegetative, mid and late crop stages were 0.6 – 0.8, 0.6 – 1.0; 1.0 – 1.05 and 0.3 - 0.4 respectively. The result showed that these crop coefficient values obtained in this experiment were similar to the crop coefficient values obtained in the past except for kc initial. Therefore, the assumption that local barley crop coefficient values differ from that of the documented values was incorrect. Furthermore, the major reason for mismanagement of irrigation water in barley fields was not due to use of wrong crop coefficient values but could be due to inadequate irrigation technical skill and knowledge of the farmer. The average economic water productivity (EWP) of barley for the very wet, wet, normal, dry and very dry seasons scenario were 0.99, 0.7, 0.65, 0.57, and 0.44 USD m-3, respectively, whereas the corresponding crop water productivity (CWP) values for grain were 1.53, 1.08, 1.0, 0.88 and 0.68 kg m-3, respectively. The EWP and CWP of barley were compared with onion and tomato under pond water irrigation at the five climatic scenarios. The crop water productivity for tomato and onion were 85 – 87% and 76 – 78% higher than that of barley, respectively. The corresponding economic water productivity for tomato and onion were 87 – 89% and 81 – 82% higher than that of barley, respectively. We concluded that growing tomato and onion would bring more income or yield per m3 of pond water supplied than growing barley. The implication is that as supply and demand determines the price of products, farmers and extension workers need to balance the crop area coverage per irrigation scheme so that undesirable price falls and rises could be avoided. Evaluation of crops based on their water productivity would improve the productivity of irrigation schemes and ultimately improve food security in the arid and semi-arid areas where water scarcity is critical problem and irrigation is a necessity for crop production. Key Words: Barley, Evapotranspiration, Crop coefficient, Water productivity

Imaging findings and management of paediatric pulmonary hydatidiosis in an Ethiopian Referral Hospital

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The Role of Endophytic Fungi in the Anticancer Activity of Morinda citrifolia

We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. The foremost goal of this study is to explore the role of endophyte-derived secondary metabolites in the purported anticancer properties of noni. To that end, culturable endophytic fungi resident within the healthy leaves and fruit of the plant were isolated and identified by molecular sequence analysis of the 5.8S gene and internal transcribed spacers (ITS). Purified organisms were subjected to in vitro fermentation in malt extract broth for 8 weeks under anaerobic conditions at room temperature (25°C), in order to simulate the conditions under which traditional fermented noni juice is prepared. The cytotoxic potential of organic extracts derived from the fermented broths of individual endophytes was then tested against three major cancers that afflict humans. Twelve distinct endophytic fungal species were obtained from the leaves and 3 from the fruit. Three of the leaf endophytes inhibited the growth of human carcinoma cell lines LU-1 (lung), PC-3 (prostate), and MCF-7 (breast) with IC50 values of ≤10 μg/mL

Podoconiosis and soil-transmitted helminths (STHs): double burden of neglected tropical diseases in Wolaita zone, rural southern Ethiopia

Background Both podoconiosis and soil-transmitted helminth (STH) infections occur among barefoot people in areas of extreme poverty; however, their co-morbidity has not previously been investigated. We explored the overlap of STH infection and podoconiosis in Southern Ethiopia and quantified their separate and combined effects on prevalent anemia and hemoglobin levels in podoconiosis patients and health controls from the same area. Methods and Principal Findings A two-part comparative cross-sectional study was conducted in Wolaita zone, southern Ethiopia. Data were collected from adult patients presenting with clinically confirmed podoconiosis, and unmatched adult neighborhood controls living in the same administrative area. Information on demographic and selected lifestyle factors was collected using interviewer-administered questionnaires. Stool samples were collected and examined qualitatively using the modified formalin-ether sedimentation method. Hemoglobin level was determined using two different methods: hemoglobinometer and automated hematology analyzer. A total of 913 study subjects (677 podoconiosis patients and 236 controls) participated. The prevalence of any STH infection was 47.6% among patients and 33.1% among controls (p<0.001). The prevalence of both hookworm and Trichuris trichiura infections was significantly higher in podoconiosis patients than in controls (AOR 1.74, 95% CI 1.25 to2.42, AOR 6.53, 95% CI 2.34 to 18.22, respectively). Not wearing shoes and being a farmer remained significant independent predictors of infection with any STH. There was a significant interaction between STH infection and podoconiosis on reduction of hemoglobin level (interaction p value = 0.002). Conclusions Prevalence of any STH and hookworm infection was higher among podoconiosis patients than among controls. A significant reduction in hemoglobin level was observed among podoconiosis patients co-infected with hookworm and ‘non-hookworm STH’. Promotion of consistent shoe-wearing practices may have double advantages in controlling both podoconiosis and hookworm infection in the study area

Prevalence of Hepatitis B surface antigen (HBsAg) among visitors of Shashemene General Hospital voluntary counseling and testing center

<p>Abstract</p> <p>Background</p> <p>Hepatitis B virus (HBV) infection is significant health problem, as it can lead to chronic hepatitis, liver cirrhosis, and hepatic carcinoma. Due to shared routes of transmission, HBV and human immunodeficiency virus (HIV) co-infection is common and is an emerging concern in the clinical management of patients because of increased mortality, accelerated hepatic disease progression, and the frequent hepatotoxicity caused by anti-retroviral therapy. The aim of this study was to determine the prevalence of Hepatitis B surface antigen (HBsAg) and its risk factors, among individuals visiting Shashemene General Hospital VCT center.</p> <p>Findings</p> <p>Institution based cross-sectional study was performed from November 3, 2008 to December 29, 2008 and 384 voluntary counseling and testing (VCT) clients were investigated. Data on socio demographic and HBV risk factors was collected using structured questionnaires. Blood samples were collected and screened for hepatitis B surface antigen (HBsAg) and HIV by commercially available rapid test kits. The prevalence of HBsAg in this study group was 5.7%. Fourteen percent of HIV positive subjects (8/57) and 4.3% (14/327) of HIV negative subjects were positive for HBsAg. Significantly high prevalence of HBsAg was observed among individuals who had history of invasive procedures, like tooth extraction, abortion and ear piercing; history of hospital admission, history of unsafe inject and HIV positives.</p> <p>Conclusions</p> <p>Although HBsAg prevalence is much higher among subjects who are HIV positive (14.0% versus 4.3%), the prevalence of HBsAg in HIV negative subjects is high enough to warrant a recommendation to screen all clients at VCT centers irrespective of HIV status.</p

Malaria protection due to sickle haemoglobin depends on parasite genotype

Host genetic factors can confer resistance against malaria1, raising the question of whether this has led to evolutionary adaptation of parasite populations. Here we searched for association between candidate host and parasite genetic variants in 3,346 Gambian and Kenyan children with severe malaria caused by Plasmodium falciparum. We identified a strong association between sickle haemoglobin (HbS) in the host and three regions of the parasite genome, which is not explained by population structure or other covariates, and which is replicated in additional samples. The HbS-associated alleles include nonsynonymous variants in the gene for the acyl-CoA synthetase family member2-4 PfACS8 on chromosome 2, in a second region of chromosome 2, and in a region containing structural variation on chromosome 11. The alleles are in strong linkage disequilibrium and have frequencies that covary with the frequency of HbS across populations, in particular being much more common in Africa than other parts of the world. The estimated protective effect of HbS against severe malaria, as determined by comparison of cases with population controls, varies greatly according to the parasite genotype at these three loci. These findings open up a new avenue of enquiry into the biological and epidemiological significance of the HbS-associated polymorphisms in the parasite genome and the evolutionary forces that have led to their high frequency and strong linkage disequilibrium in African P. falciparum populations

Simulating Potential Impacts of Solar MajiPump on the Economy and Nutrition of Smallholder Farmers in Sub-Humid Ethiopia

Irrigation is widely considered a potential means to improve agricultural productivity, nutrition, and income, as farmers can carry out farming and production year-round. However, the feasibility of irrigation technologies is highly dependent on the long-term economic return farmers achieve. Solar-based irrigation could address the challenges of underinvestment in irrigation within Africa. Evidence on the economic viability of the adopted solar pumps such as MajiPump is very scant and focused on ex post evaluation. This study evaluated the income and nutritional feasibility of solar-powered irrigation using the MajiPump in sub-humid Ethiopian highlands using the farm simulation (FARMSIM) model and compared it with the manual pulley system. Results from the FARMSIM model show that farmers’ adoption of Maji solar pump technology to grow vegetables is economically feasible with financial support such as credit or loan for initial and capital investment to acquire the pump. The average profit under the solar MajiPump, drip irrigation, and conservation agriculture was 3.6 times higher than that of the baseline scenario. While the pulley technology provides the same amount of irrigation water to grow vegetables, its feasibility is limited due to high labor costs and time, estimated to be more than seven times the baseline. The simulation results show that the alternative scenarios’ nutrition level has improved relative to other scenarios and met the minimum daily average nutrition requirement level for proteins, iron, and vitamin A but fell short in fat, calcium, and calories. The results suggest that farmers who adopt improved small-scale irrigation technologies (solar MajiPump and drip system) have a higher potential to increase production and income from irrigated crops and improve their nutrition if part of the income generated is used to purchase supplemental food for their nutrition

Changing risk of environmental Campylobacter exposure with emerging poultry production systems in Ethiopia

Campylobacter is a leading cause of diarrhoea, and its presence in chickens is a significant risk for zoonotic infection. Poultry production is becoming increasingly intensive in Ethiopia and is incorporating more high-producing breeds into traditionally managed smallholdings, especially in peri-urban areas. This cross-sectional study sampled 219 household environments in one peri-urban and two rural areas of Ethiopia, and an additional 20 semi-intensive farms in the peri-urban district. Campylobacter was detected by polymerase chain reaction (PCR)-specific assays in 44 samples; 16 of which could be identified as C. jejuni. Flocks in the peri-urban area were at significantly greater odds of detection, including those which only kept indigenous birds under a scavenging system. It was also noted that scavenging flocks of exotic high-production birds (Rhode Island Red) were at slightly greater risk, perhaps as exotic birds are under more stress when kept under traditional management systems. We suggest that changes to the system of chicken production may alter the ecology and epidemiology of Campylobacter in the environment, chickens and people, which may drive emergence of new epidemiological patterns of disease. Further research is needed to determine the extent to which the current management intensification and the distribution programmes of exotic and/or improved indigenous birds may alter Campylobacter epidemiology, ecology and public health risk, before their widespread adoption

Mental health and quality of life of women one year after maternal near-miss in low and middle-income countries: The case of zanzibar, Tanzania

Women who experienced a maternal near-miss are at risk of mental health complications and lower quality of life, but long-term consequences are largely unknown. The aim of this study is to assess whether mental health symptoms and quality of life change over time and to examine associations with risk factors among post-partum women. In this cohort study, women with maternal near-miss were matched to women without or with mild complications at Mnazi Mmoja Hospital in Zanzibar. Depressive and post-traumatic stress disorder symptoms, and quality of life were measured at three, six, and twelve-months follow-up. A linear mixed-effects model was used for data analysis. Postpartum women in Zanzibar reported low levels of depressive and post-traumatic stress disorder symptoms. While depressive symptoms and quality of life trajectories were similar among women with and without maternal near-miss, differences for trajectories of post-traumatic stress disorder symptoms and physical quality of life were found. Social support, perinatal loss, and intercurrent illness were strongly associated with both depressive symptoms and quality of life in this group of Islamic women. These findings suggest that social support, embedded in the cultural context, should be considered in helping women cope with mental health issues in the aftermath of severe maternal complications