Influence of the biologically active dietary supplement Floravit® on the skin and hair structure in sable (Martes zibellina)

It is important to search for new highly effective multicomponent compounds that are able to influence several of animals’ homeostasis systems simultaneously to improve the physiological adaptation of fur animals to different conditions of nutrition. This is the function of the feed additive Floravit® – a natural bioregulator. The compound is a combination of biologically active ingredients produced by the mycelial fungus Fusarium sambucinum. Studied was the effect of Floravit® on the structure of the skin and hair in adult female sable (Martes zibellina). The scientific and economic experiment was conducted at the JSC “Plemzavod Pushkinskyi” in the Moscow Region during the period of winter fur formation in October-November. The study of the morphological structure of the hair and skin cover was carried out in the chine, side and rump topographical areas. The structure of the guard hairs in the main topographical areas was examined on a scanning electron microscope. The experiment showed that administration of Floravit® per os to adult female sable at a dose of 1.0 ml per head per day throughout the period of winter pelt formation in October-November has an influence on the morphological structure of all the categories of hair on all topographic pelt areas. Animals in the test group exposed to Floravit® exhibited an increase in guide hair length on the chine and side, when compared to controls, by 4.1 mm (p < 0.001) and by 2.8 mm (p < 0.01), respectively. The length of guard hair on the chine, side and rump increased by 8.1, 7.8 and 7.8 mm (p < 0.001), respectively. An increase in down hair length was recorded in all areas of the pelt, when compared to controls, by 13.0, 4.5 and 6.3 mm (p < 0.001). An increase in dermal thickness was recorded in the chine area by 0.7 mm (p < 0.001). The specified changed in the skin and hair structure in sable adult females after using Floravit® have shown a positive influence on the quality of hair cover. As a result, bioregulator Floravit® takes part in the adaptation process of the sable organism to external factors

Genome sequences of Acholeplasma laidlawii strains differing in sensitivity to ciprofloxacin

© 2017 Medvedeva et al. Acholeplasma laidlawii is a well-suited model for study of the molecular basis of the adaptation of mollicutes to environmental conditions. Here we present the whole-genome sequences of four strains of A. laidlawii with differential sensitivity to ciprofloxacin

Selecting and structuring teaching content algorithm for physical and mathematical disciplines, aimed at students’ project-technical competence formation

© 2015, Review of European Studies. All right reserved. The most important component of the professional competence of an engineer is the synthesis of project and technical competences—project-technical competence, suggesting a set of special knowledge, skills and proficiencies, personal qualities necessary for the effective specialists’ performance of their professional duties, in particular, for the successful implementation of engineering projects requiring technical calculations running, capabilities to effectively operate in real life, to get oriented in production situations, to adapt to changing conditions, to work in different teams. In this connection, the problem of selecting and structuring teaching content of physical and mathematical disciplines, aimed at the formation of project-technical competence of technical university students, is becoming relevant. The paper presents an algorithm for selecting and structuring the physical and mathematical disciplines teaching content, aimed at the formation of students’ project-technical competence, involving a sequence of analytical and preparatory, organizational-substantive, procedural-methodological, as well as correction-implemental stages. The article submissions are of practical value to Mathematics and Physics teachers for selecting and structuring the content of these disciplines

Pollen quality and pollen productivity of blue honeysuckle species and varieties

Studies to assess the pollen quality and pollen productivity of blue honeysuckle were conducted on the ecosystem dendrological territory of the Siberian Botanical garden of TSU (Tomsk). Objects of research: 8 varieties and 4 species of blue honeysuckle – ‘Velvet’, ‘Berel’, ‘Vasyuganskaya’, ‘Zolushka’, ‘Lazurnaya’, ‘Ogneny Opal’, ‘Selena’, ‘Tomichka’, Lonicera altaica, L. edulis, L. kamtschatica, L. turczaninovii. It was found that the ‘Ogneny Opal’ and ‘Velvet’ varieties have low fertility, and the ‘Berel’ and L. edulis have an average fertility. Other varieties and species of honeysuckle have high pollen fertility. The viability of pollen with high values is more than 60 % – ‘Berel’, ‘Vasyuganskaya’, ‘Lazurnaya’, L. turczaninovii, L. kamtschatica, with average values from 40% to 60 % – ‘Selena’, ‘Tomichka’ and L. altaica, with low values – less than 40 % – ‘Velvet’, ‘Zolushka’, ‘Ogneny Opal’ and L. edulis. High pollen productivity – more than 20,000 pollen grains per flower – ‘Tomichka’, L. kamtschatica, L. turczaninovii and L. edulis, average productivity - from 10,000 to 20,000 pollen grains – ‘Berel’, ‘Vasyuganskaya’, ‘Zolushka’, ‘Lazurnaya’, ‘Selena’ and L. altaica, low productivity – less than 10,000 pollen grains per flower – ‘Velvet’, ‘Ogneny Opal’. It is recommended to use at least 10% of varieties with high pollen viability and pollen productivity as pollinators when creating industrial honeysuckle plantations: ‘Lazurnaya’, ‘Vasyuganskaya’, and ‘Berel’

Language and cultural code peculiarities within the framework of crosscultural communication

The article deals with the peculiarities of linguistic and cultural code-specific features which have a significant impact on the successful communication process within the framework of intercultural interaction. According to the research work, the basic ground of cross-cultural communication operates with definitions and cultural elements, which are of primary importance in the language-mastering process and adaptation procedure to the foreign language environmen

Adaptation of mycoplasmas to antimicrobial agents: Acholeplasma laidlawii extracellular vesicles mediate the export of ciprofloxacin and a mutant gene related to the antibiotic target

This study demonstrated that extracellular membrane vesicles are involved with the development of resistance to fluoroquinolones by mycoplasmas (class Mollicutes). This study assessed the differences in susceptibility to ciprofloxacin among strains of Acholeplasma laidlawii PG8. The mechanisms of mycoplasma resistance to antibiotics may be associated with a mutation in a gene related to the target of quinolones, which could modulate the vesiculation level. A. laidlawii extracellular vesicles mediated the export of the nucleotide sequences of the antibiotic target gene as well as the traffic of ciprofloxacin. These results may facilitate the development of effective approaches to control mycoplasma infections, as well as the contamination of cell cultures and vaccine preparations. © 2014 Elena S. Medvedeva et al

Antimicrobial resistance in mollicutes: Known and newly emerging mechanisms

© FEMS 2018. All rights reserved. This review is devoted to the mechanisms of antibiotic resistance in mollicutes (class Bacilli, subclass Mollicutes), the smallest self-replicating bacteria, that can cause diseases in plants, animals and humans, and also contaminate cell cultures and vaccine preparations. Research in this area has been mainly based on the ubiquitous mollicute and the main contaminant of cell cultures, Acholeplasma laidlawii. The omics technologies applied to this and other bacteria have yielded a complex picture of responses to antimicrobials, including their removal from the cell, the acquisition of antibiotic resistance genes and mutations that potentially allow global reprogramming of many cellular processes. This review provides a brief summary of well-known resistance mechanisms that have been demonstrated in several mollicutes species and, in more detail, novel mechanisms revealed in A. laidlawii, including the least explored vesicle-mediated transfer of short RNAs with a regulatory potency. We hope that this review highlights new avenues for further studies on antimicrobial resistance in these bacteria for both a basic science and an application perspective of infection control and management in clinical and research/production settings

Бабезиоз у человека (лекция)

Babesiosis is a natural focal infectious disease caused by protozoa of the genus Babesia, transmitted transmissibly and characterized by fever, intoxication, progressive hemolytic anemia, and often severe course in immunocompromised patients. The clinical picture of babesiosis is nonspecific. The disease should be suspected in patients with unexplained fever who have been in endemic areas for 2 months or have received a blood transfusion within 6 months. Diagnosis of babesiosis is difficult; depends on the qualifications of the doctor and the number of parasites in the blood. The aim of the work is to summarize the available information about the etiology, epidemiology, pathogenesis, clinic, diagnosis, etiotropic therapy and prevention of babesiosis in humans. A review of domestic and foreign literature over the past 30 years, including Internet resources, has been carried out.Бабезиоз — природно-очаговое инфекционное заболевание, вызываемое простейшими рода Babesia, передающееся трансмиссивным путем и характеризующееся лихорадкой, интоксикацией, прогрессирующей гемолитической анемией и часто тяжелым течением у пациентов с иммунодефицитными состояниями. Клиническая картина бабезиоза неспецифична. Заболевание следует заподозрить у пациентов с необъяснимой лихорадкой, которые находились в эндемичных районах в течение 2 месяцев или получили переливание крови в течение 6 месяцев. Диагностика бабезиоза сложна; зависит от квалификации врача и от количества паразитов в крови. Целью работы является обобщение имеющихся сведений об этиологии, эпидемиологии, патогенезе, клинике, диагностике, этиотропной терапии и профилактике бабезиоза у человека. Проведен обзор отечественной и зарубежной литературы за последние 30 лет, в том числе Интернет-ресурсов

Genomic and proteomic profiles of Acholeplasma laidlawii strains differing in sensitivity to ciprofloxacin

© 2016, Pleiades Publishing, Ltd.As a result of comparative analysis of complete genomes as well as cell and vesicular proteomes of A. laidlawii strains differing in sensitivity to ciprofloxacin, it was first shown that the mycoplasma resistance to the antibiotic is associated with the reorganization of genomic and proteomic profiles, which concerns many genes and proteins involved in fundamental cellular processes and realization of bacterial virulence