Src Kinase Determines the Dynamic Exchange of the Docking Protein NEDD9 (Neural Precursor Cell Expressed Developmentally Down-regulated Gene 9) at Focal Adhesions

Dynamic exchange of molecules between the cytoplasm and integrin-based focal adhesions provides a rapid response system for modulating cell adhesion. Increased residency time of molecules that regulate adhesion turnover contributes to adhesion stability, ultimately determining migration speed across two-dimensional surfaces. In the present study we test the role of Src kinase in regulating dynamic exchange of the focal adhesion protein NEDD9/HEF1/Cas-L. Using either chemical inhibition or fibroblasts genetically null for Src together with fluorescence recovery after photobleaching (FRAP), we find that Src significantly reduces NEDD9 exchange at focal adhesions. Analysis of NEDD9 mutant constructs with the two major Src-interacting domains disabled revealed the greatest effects were due to the NEDD9 SH2 binding domain. This correlated with a significant change in two-dimensional migratory speed. Given the emerging role of NEDD9 as a regulator of focal adhesion stability, the time of NEDD9 association at the focal adhesions is key in modulating rates of migration and invasion. Our study suggests that Src kinase activity determines NEDD9 exchange at focal adhesions and may similarly modulate other focal adhesion-targeted Src substrates to regulate cell migration.NHMRC Grant 63251

Spitzer Observations of Low Luminosity Isolated and Low Surface Brightness Galaxies

We examine the infrared properties of five low surface brightness galaxies (LSBGs) and compare them with related but higher surface brightness galaxies, using Spitzer Space Telescope images and spectra. All the LSBGs are detected in the 3.6 and 4.5um bands, representing the stellar population. All but one are detected at 5.8 and 8.0um, revealing emission from hot dust and aromatic molecules, though many are faint or point-like at these wavelengths. Detections of LSBGs at the far-infrared wavelengths, 24, 70, and 160um, are varied in morphology and brightness, with only two detections at 160um, resulting in highly varied spectral energy distributions. Consistent with previous expectations for these galaxies, we find that detectable dust components exist for only some LSBGs, with the strength of dust emission dependent on the existence of bright star forming regions. However, the far-infrared emission may be relatively weak compared with normal star-forming galaxies.Comment: 20 pages, 8 figures, accepted to Ap

Creating and studying ion acoustic waves in ultracold neutral plasmas

We excite ion acoustic waves in ultracold neutral plasmas by imprinting density modulations during plasma creation. Laser-induced fluorescence is used to observe the density and velocity perturbations created by the waves. The effect of expansion of the plasma on the evolution of the wave amplitude is described by treating the wave action as an adiabatic invariant. After accounting for this effect, we determine that the waves are weakly damped, but the damping is significantly faster than expected for Landau damping

Predictors of Functional Outcomes following Operative Treatment of Acute Achilles Tendon Ruptures

Introduction: Previous studies involving operative management of Achilles tendon ruptures have attempted to determine if patient factors influence outcomes. No previous study has attempted to identify outcome predictors in patients exclusively undergoing surgical repair. The purpose of this study is to determine if any injury or patient variables were predictive of outcomes following operative management of Achilles ruptures. Methods: Patient demographics including age, sex, body mass index (BMI), comorbidities (diabetes mellitus, depression, anxiety), mechanism of injury (sports, non-sports), and date of injury were collected. Postoperative notes were reviewed to determine compliance. Patients completed the Foot & Ankle Ability Measure (FAAM)-Activities of Daily Living (ADL) and –Sports subscales, and visual analog scale (VAS) for pain. Multivariable regression analysis was performed, and regression coefficients with 95% confidence intervals and p-values were reported. Results: Female sex was associated with lower FAAM-Sports score (-10.11 [-19.73,-0.50]) and a lower Single Assessment Numeric Evaluation score from the FAAM-Sports subscale (-13.79 [-26.28,-1.30]; p=0.0325). History of anxiety was related to a lower FAAM-ADL score (-29.02 [-45.68, -12.36]; p=0.0009), FAAM-Sports score (-33.41 [-64.46, -2.37]; p=0.0368), and a higher VAS pain score (19.83 [4.43, 35.23]; p=0.0128). Age, BMI, a history of depression or diabetes mellitus, mechanism of injury, timing of repair, and patient compliance were not predictive. Discussion: Females and patients with anxiety have significantly poorer outcomes following Achilles tendon repair. Further study is indicated to determine whether these factors are also predictive of outcomes of Achilles ruptures treated non-surgically and how this may affect surgical indications in these patients

Optical microrheology using rotating laser-trapped particles

We demonstrate an optical system that can apply and accurately measure the torque exerted by the trapping beam on a rotating birefringent probe particle. This allows the viscosity and surface effects within liquid media to be measured quantitatively on a micron-size scale using a trapped rotating spherical probe particle. We use the system to measure the viscosity inside a prototype cellular structure.Comment: 5 pages, 4 figures. v2: bibliographic details, minor text correction

Long-time discrete particle effects versus kinetic theory in the self-consistent single-wave model

The influence of the finite number N of particles coupled to a monochromatic wave in a collisionless plasma is investigated. For growth as well as damping of the wave, discrete particle numerical simulations show an N-dependent long time behavior resulting from the dynamics of individual particles. This behavior differs from the one due to the numerical errors incurred by Vlasov approaches. Trapping oscillations are crucial to long time dynamics, as the wave oscillations are controlled by the particle distribution inhomogeneities and the pulsating separatrix crossings drive the relaxation towards thermal equilibrium.Comment: 11 pages incl. 13 figs. Phys. Rev. E, in pres

A Simple Analytical Model of the Angular Momentum Transformation in Strongly Focused Light Beams

A ray-optics model is proposed to describe the vector beam transformation in a strongly focusing optical system. In contrast to usual approaches basing on the focused field distribution near the focal plane, we employ the transformed beam pattern formed immediately near the exit pupil. In this cross section, details of the output field distribution are of minor physical interest but proper allowance is made for transformation of the incident beam polarization state. This enables to obtain the spin and orbital angular momentum representations which are valid everywhere in the transformed beam space. Simple analytical results are available for the transversely homogeneous circularly polarized incident beam limited only by the circular aperture. Behavior of the spin and orbital angular momenta of the output beam and their dependences on the focusing strength (aperture angle) are analyzed. The obtained analytical results are in good qualitative and reasonable quantitative agreement to the calculation performed for the spatially inhomogeneous Gaussian and Laguerre-Gaussian beams. In application to Laguerre-Gaussian beams, the model provides possibility for analyzing the angular momentum transformation in beams already possessing some mixture of the spin and orbital angular momenta. The model supplies efficient and physically transparent means for qualitative analysis of the spin-to-orbital angular momentum conversion. It can be generalized to incident beams with complicated spatial and polarization structure.Comment: 18 pages, 5 figures. The paper has appeared as an attempt to clearly understand transformations of the light beam polarization in the course of strong focusing. It provides description of the optical vortex formation after focusing a circularly polarized beam and explains why the the orbital angular momentum emerges in the focused bea

Asymptotic stability, concentration, and oscillation in harmonic map heat-flow, Landau-Lifshitz, and Schroedinger maps on R^2

We consider the Landau-Lifshitz equations of ferromagnetism (including the harmonic map heat-flow and Schroedinger flow as special cases) for degree m equivariant maps from R^2 to S^2. If m \geq 3, we prove that near-minimal energy solutions converge to a harmonic map as t goes to infinity (asymptotic stability), extending previous work down to degree m = 3. Due to slow spatial decay of the harmonic map components, a new approach is needed for m=3, involving (among other tools) a "normal form" for the parameter dynamics, and the 2D radial double-endpoint Strichartz estimate for Schroedinger operators with sufficiently repulsive potentials (which may be of some independent interest). When m=2 this asymptotic stability may fail: in the case of heat-flow with a further symmetry restriction, we show that more exotic asymptotics are possible, including infinite-time concentration (blow-up), and even "eternal oscillation".Comment: 34 page

Adaptability of irrigated spring canola oil production to the US High Plains

Canola oil is high in oleic acid which is commonly used for food and industrial purposes. To determine adaptability of spring canola (Brassica napus L.) to the High Plains for industrial oil production, 26 irrigated trials were conducted from 2005 to 2008. Trials were divided into five regions—1: 36–37◦N 108◦W; 2: 39–40◦N 101–103◦W; 3: 41–42◦N 102–103◦W; 4: 41–42◦N 104◦W; 5: 43–44◦N 106–108◦W. Cultural practices were based on site-specific protocols. Four cultivars, Hyola 401, Hyola 357 Magnum, SW Marksman, and SW Patriot, were planted in replicated plots in April or May under standard irrigation and harvested in July to October depending on region. Seed yield Hyola 401 and Hyola 357 Magnum were higher than SW Marksman and SW Patriot across the five regions and within Regions 1, 2, 3, and 5. Regions 1, 2 and 3 yielded significantly greater than did Regions 4 and 5. Samples from 18 trials were examined for their oil content and fatty acid distribution. The four cultivars had greater than 38% oil content; SW Marksman and SW Patriot had higher oil content than Hyola 401 and Hyola 357 Mag. Higher oil content was achieved in Regions 1, 4 and 5. Across and within regions, the percent of oleic acid did not differ for the four cultivars. The mean content of oleic acid decreased going north from Region 2 to Region 5, as did seed yield in the High Plains. Linoleic acid increased going north from Region 1. Linolenic acids showed little variation across regions. Considering yield and total oil content together, growing spring canola would be excellent in the High Plains