Driving down the Detection Limit in Microstructured Fiber-Based Chemical Dip Sensors

We present improvements to fluorescence sensing in soft-glass microstructured optical fibers that result in significantly improved sensitivity relative to previously published results. Concentrations of CdSe quantum dots down to 10 pM levels have been demonstrated. We show that the primary limitation to the sensitivity of these systems is the intrinsic fluorescence of the glass itself

Cancer detection in human tissue samples using a fiber-tip pH probe

Abstract not availableErik P. Schartner, Matthew R. Henderson, Malcolm Purdey, Deepak Dhatrak, Tanya M. Monro, P. Grantley Gill, and David F. Calle

Advances and limitations in the modelling of fabricated photonic bandgap fibers

Copyright © 2006 IEEEWe model fabricated silica photonic bandgap fibers and achieve good agreement between simulated and measured properties. We identify the size of the SEM bitmap image as the ultimate limit to the accurate calculation of surfaces modes within the bandgapF. Poletti, M. N. Petrovich, R. Amezcua-Correa, N. G. Broderick, T. M. Monro and D. J. Richardsonhttp://eprints.soton.ac.uk/47883

Q-factor limits for far-field detection of whispering gallery modes in active microspheres

Abstract not availableNicolas Riesen,Tess Reynolds, Alexandre François, Matthew R. Henderson, and Tanya M. Monr

Enhancing the radiation efficiency of dye doped whispering gallery mode microresonators

We present a novel form of a Whispering Gallery Mode (WGM) sensor that exploits dye doped polystyrene microspheres, as active resonators, positioned onto the tip of a Microstructured Optical Fiber (MOF) as a means of overcoming the limited Q-factors for small resonators. We show that it is possible to substantially enhance the fluorescence emission of selected WGMs of the microspheres, resulting in an increase of the signal-to-noise ratio of the modes and of the effective Q-factor. This is done by positioning the resonator into one of the holes of a suspended core MOF and matching the resonator diameter with the hole diameter where it sits, effectively breaking the symmetry of the environment surrounding the sphere. Furthermore we demonstrate that using this experimental configuration, the lasing efficiency of the dye-doped microspheres is also significantly enhanced, which also contributes to an enhancement in the observed Q-factor.Alexandre François, Kristopher J. Rowland, Shahraam Afshar V., Matthew R. Henderson, and Tanya M. Monr

Combining whispering gallery mode lasers and microstructured optical fibers: limitations, applications and perspectives for in-vivo biosensing

Published online: 12 May 2016Whispering gallery modes (WGMs) have been widely studied over the past 20 years for various applications, including biological sensing. While the WGM-based sensing approaches reported in the literature have shown tremendous performance down to single molecule detection, at present such sensing technologies are not yet mature and still have significant practical constraints that limit their use in real-world applications. Our work has focused on developing a practical, yet effective, WGM-based sensing platform capable of being used as a dip sensor for in-vivo biosensing by combining WGM fluorescent microresonators with silica Microstructured Optical Fibers (MOFs). We recently demonstrated that a suspended core MOF with a dye-doped polymer microresonator supporting WGMs positioned onto the tip of the fiber, can be used as a dip sensor. In this architecture the resonator is anchored to one of the MOF air holes, in contact with the fiber core, enabling a significant portion of the evanescent field from the fiber to overlap with the sphere and hence excite the fluorescent WGMs. This architecture allows for remote excitation and collection of the WGMs. The fiber also permits easy manipulation of the microresonator for dip sensing applications, and hence alleviates the need for a complex microfluidic interface. More importantly, it allows for an increase in both the excitation and collection efficiency compared to free space coupling, and also improves the Q factor. In this paper we present our recent results on microstructured fiber tip WGM-based sensors and show that this sensing platform can be used in clinical diagnostics, for detecting various clinically relevant biomarkers in complex clinical samples.Alexandre François, Tess Reynolds, Nicolas Riesen, Jonathan M. M. Hall, Matthew R. Henderson, Enming Zhao, Shahraam Afshar V. and Tanya M. Monr

Fifty percent internal slope efficiency femtosecond direct-written Tm(3+):ZBLAN waveguide laser

We report a 790 nm pumped, Tm³⁺ doped ZBLAN glass buried waveguide laser that produces 47 mW at 1880 nm, with a 50% internal slope efficiency and an M² of 1.7. The waveguide cladding is defined by two overlapping rings created by femtosecond direct-writing of the glass, which results in the formation of a tubular depressed-index-cladding structure, and the laser resonator is defined by external dielectric mirrors. This is, to the best of our knowledge, the most efficient laser created in a glass host via femtosecond waveguide writing.D. G. Lancaster, S. Gross, H. Ebendorff-Heidepriem, K. Kuan, T. M. Monro, M. Ams, A. Fuerbach, and M. J. Withfor

Considering Intra-individual Genetic Heterogeneity to Understand Biodiversity

In this chapter, I am concerned with the concept of Intra-individual Genetic Hetereogeneity (IGH) and its potential influence on biodiversity estimates. Definitions of biological individuality are often indirectly dependent on genetic sampling -and vice versa. Genetic sampling typically focuses on a particular locus or set of loci, found in the the mitochondrial, chloroplast or nuclear genome. If ecological function or evolutionary individuality can be defined on the level of multiple divergent genomes, as I shall argue is the case in IGH, our current genetic sampling strategies and analytic approaches may miss out on relevant biodiversity. Now that more and more examples of IGH are available, it is becoming possible to investigate the positive and negative effects of IGH on the functioning and evolution of multicellular individuals more systematically. I consider some examples and argue that studying diversity through the lens of IGH facilitates thinking not in terms of units, but in terms of interactions between biological entities. This, in turn, enables a fresh take on the ecological and evolutionary significance of biological diversity

Integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre for DNA probe immobilization

In this paper, we demonstrate the integration of a 3D hydrogel matrix within a hollow core photonic crystal fibre (HC-PCF). In addition, we also show the fluorescence of Cy5-labelled DNA molecules immobilized within the hydrogel formed in two different types of HC-PCF. The 3D hydrogel matrix is designed to bind with the amino groups of biomolecules using an appropriate cross-linker, providing higher sensitivity and selectivity than the standard 2D coverage, enabling a greater number of probe molecules to be available per unit area. The HC-PCFs, on the other hand, can be designed to maximize the capture of fluorescence to improve sensitivity and provide longer interaction lengths. This could enable the development of fibre-based point-of-care and remote systems, where the enhanced sensitivity would relax the constraints placed on sources and detectors. In this paper, we will discuss the formation of such polyethylene glycol diacrylate (PEGDA) hydrogels within a HC-PCF, including their optical properties such as light propagation and auto-fluorescence