SIRTF Telescope Instrument Changeout and Cryogen Replenishment (STICCR) Study

The Space Infrared Telescope Facility (SIRTF) is a long-life cryogenically cooled space-based telescope for infrared astronomy from 2 to 700 micrometers. SIRTF is currently under study by NASA-ARC (Reference AP) and planned for launch in approximately the mid 1990s. SIRTF will operate as a multiuser facility, initially carrying three instruments at the focal plane. It will be cooled to below 2 K by superfluid liquid helium to achieve radiometric sensitivity limited only by the statistical fluctuations in the natural infrared background radiation over most of its spectral range. The lifetime of the mission will be limited by the lifetime of the liquid helium supply, and baseline is currently to be 2 years. The telescope changes required to allow in-space replenishment of the 4,000-L superfluid helium tank was investigated. A preliminary design for the space services equipment was also developed. The impacts of basing the equipment and servicing on the space station were investigated. Space replenishment and changeout of instruments required changes to the telescope design. Preliminary concepts are presented

Immunohistochemical Demonstration of IgG in Reed-Sternberg and Other Cells in Hodgkin\u27s Disease

Increased synthesis of IgG in vitro has been demonstrated in spleens from patients with Hodgkin\u27s disease, either with or without invasion of the organ by tumor (1). Interest in this laboratory has centered recently on cytochemical localization of immunoglobulins by means of an immunoglobulin-peroxidase bridge procedure (2) and a satisfactory method has been developed for selectively visualizing immunocytes with this technique. 1 As a means of assessing the basis for increased IgG biosynthesis in spleens of Hodgkin patients, this immunostaining procedure has been applied to localization of IgG-producing cells in specimens with Hodgkin\u27s disease

Effects of environmental exposure on cryogenic thermal insulation materials

Investigation was made to optimize selection of insulation materials for reusable space vehicles which will be repeatedly operated over periods of up to ten years. Results of study are summarized in two reports. Volume I describes tests and significant findings. In Volume II, extensive test data obtained are organized in handbook form

A four-season study quantifying the weekly external training loads during different between match microcycle lengths in professional rugby league

This study investigated differences in external training load between microcycle lengths and its variation between microcycles, players, and head coaches. Commonly used external training load variables including total-, high-speed- (5-7 m∙s-1), and sprint-distance (> 7 m∙s-1) alongside combined high acceleration and deceleration distance (> 2 m∙s-2). Which were also expressed relative to time were collected using microtechnology within a repeated measures design from 54 male rugby league players from one Super League team over four seasons. 4337 individual observations across ninety-one separate microcycles and six individual microcycle lengths (5 to 10 day) were included. Linear mixed effects models established the differences in training load between microcycle-length and the variation between-microcycles, players and head coaches. The largest magnitude of difference in training load was seen when comparing 5-day with 9-day (ES = 0.31 to 0.53) and 10-day (ES = 0.19 to 0.66) microcycles. The greatest number of differences between microcycles were observed in high- (ES = 0.3 to 0.53) and sprint-speed (ES = 0.2 to 0.42) variables. Between-microcycle variability ranged between 11% to 35% dependent on training load variable. Training load also varied between players (5-65%) and head coaches (6-20%) with most variability existing within high-speed (19-43%) and sprinting (19-65%). Overall, differences in training load between microcycle lengths exist, likely due to manipulation of session duration. Furthermore, training load varies between microcycle, player and head coach

Ultrastructural morphology and cytochemistry of iron-deficient polymorphonuclear leukocytes

Previous studies have documented decreased activities of certain enzymes and altered function in polymorphonuclear leukocytes (PMN) during iron deficiency. The present study was undertaken to determine if the enzymatic abnormalities could be correlated with morphologic or quantitative change in PMN granules. Ultrastructural examination of primary and secondary granules and assessment of the secondary granule components alkaline phosphatase and vicinal glycol-containing glycoconjugates was performed in rabbit bone marrow, peripheral blood, and peritoneal heterophils. In addition, biochemical quantifications of the secondary granule component alkaline phosphatase and the primary granule marker [beta]-glucuronidase were performed. The results confirmed that a marked, significant decrease in alkaline phosphatase occurs in iron-deficient animals; however, no biochemical decrease in [beta]-glucuronidase activity was observed. Ultrastructurally, PMN secondary granules of iron-deficient rabbits tended to be more numerous than in controls when examined with morphometric and glycoconjugate staining methods, but lacked staining in alkaline phosphatase preparations. These results demonstrate that iron-deficient rabbits produce normal to increased quantities of primary and secondary granules, despite a uniform deficiency of alkaline phosphatase, a secondary granule marker.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26267/1/0000352.pd

The speed and acceleration of the ball carrier and tackler into contact during front-on tackles in rugby league.

The aim was to use a combination of video analysis and microtechnology (10 Hz global positioning system [GPS]) to quantify and compare the speed and acceleration of ball-carriers and tacklers during the pre-contact phase (contact - 0.5s) of the tackle event during rugby league match-play. Data were collected from 44 professional male rugby league players from two Super League clubs across two competitive matches. Tackle events were coded and subject to three stages of inclusion criteria to identify front-on tackles. 10 Hz GPS data was synchronised with video to extract the speed and acceleration of the ball-carrier and tackler into each front-on tackle (n = 214). Linear mixed effects models (effect size [ES], confidence intervals, p-values) compared differences. Overall, ball-carriers (4.73 ± 1.12 m∙s-1) had greater speed into front-on tackles than tacklers (2.82 ± 1.07 m∙s-1; ES = 1.69). Ball-carriers accelerated (0.67 ± 1.01 m∙s-2) into contact whilst tacklers decelerated (-1.26 ± 1.36 m∙s-2; ES = 1.74). Positional comparisons showed speed was greater during back vs. back (ES = 0.66) and back vs. forward (ES = 0.40) than forward vs. forward tackle events. Findings can be used to inform strategies to improve performance and player welfare

p16 Overexpression: A Potential Early Indicator of Transformation in Ovarian Carcinoma

Objective: The recently cloned gene p16 (MST 1) has been identified as a putative tumor suppressor gene that binds to CDK4 and CDK6 (cyclin-dependent kinases), preventing their interaction with cyclin D1 and thereby preventing cell cycle progression at the G1 stage. In addition, the p16 gene has been shown to have a high frequency of mutation in some tumor cell lines; however, it has also been shown that a much lower frequency of mutation occurs in primary tumors. This study investigated the mRNA expression level and mutation status of the p16 gene in ovarian tumors. Methods: We performed quantitative polymerase chain reaction and direct cDNA sequencing analysis. To confirm the p16 protein level in ovarian tumors, Western blotting and immunohistochemical staining were performed. Expression levels of mRNA for the p16 gene relative to the β-tubulin gene were examined in 32 ovarian tumors (24 carcinomas, six low malignant potential tumors, and two benign tumors) and six normal ovaries. Results: The mRNA expression level of p16 was significantly elevated in 28 ovarian tumors (22 carcinomas, five low malignant potential tumors, and one benign tumor) compared with that of normal ovaries. Western blotting analysis and immunohistochemical staining confirmed elevated p16 protein levels in ovarian tumor samples. Among 32 ovarian tumors, cDNA sequencing of the p16 gene showed no p16 mutation resulting in a coding error, although one silent mutation and three polymorphisms were found. Conclusions: Although p16 is seldom mutated in ovarian tumors, the overexpression of p16 in most ovarian tumor cases indicates a dysfunction in the regulatory complex for G1 arrest. Therefore, overexpression of p16 may be an important early event in the neoplastic transformation of the ovarian epithelium.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68382/2/10.1177_107155769700400209.pd

Depletion of somatic mutations in splicing-associated sequences in cancer genomes

Abstract Background An important goal of cancer genomics is to identify systematically cancer-causing mutations. A common approach is to identify sites with high ratios of non-synonymous to synonymous mutations; however, if synonymous mutations are under purifying selection, this methodology leads to identification of false-positive mutations. Here, using synonymous somatic mutations (SSMs) identified in over 4000 tumours across 15 different cancer types, we sought to test this assumption by focusing on coding regions required for splicing. Results Exon flanks, which are enriched for sequences required for splicing fidelity, have ~ 17% lower SSM density compared to exonic cores, even after excluding canonical splice sites. While it is impossible to eliminate a mutation bias of unknown cause, multiple lines of evidence support a purifying selection model above a mutational bias explanation. The flank/core difference is not explained by skewed nucleotide content, replication timing, nucleosome occupancy or deficiency in mismatch repair. The depletion is not seen in tumour suppressors, consistent with their role in positive tumour selection, but is otherwise observed in cancer-associated and non-cancer genes, both essential and non-essential. Consistent with a role in splicing modulation, exonic splice enhancers have a lower SSM density before and after controlling for nucleotide composition; moreover, flanks at the 5’ end of the exons have significantly lower SSM density than at the 3’ end. Conclusions These results suggest that the observable mutational spectrum of cancer genomes is not simply a product of various mutational processes and positive selection, but might also be shaped by negative selection