Play in the School Context? The Perspectives of Finnish Teachers

Playful learning environments (PLEs) have been constructed in schoolyards in Finland with the aim of increasing learning through play in curriculum-based education. In order to better understand and inform this development, the Hyvönen sets out to ascertain how teachers view and use play in kindergarten and elementary education. Fourteen teachers were interviewed, and the data obtained were analyzed using the grounded theory approach. Eight play types were distinguished, with the teacher having the roles of leader, allower, and afforder. Play types were found to be either curriculum-driven, or seen as facilitating friendship or integrating play and learning as a process. ‘Playful teaching’ was characterized in terms of the roles assumed by teachers and students in different play situations, the design of playful learning processes, the emphasis on developing and using children’s creativity, and the importance of fun and enjoyment. The Hyvönen concludes that teacher education should develop teachers’ pedagogical thinking through the theoretical understanding of play and learning, as well as through discussions and the modeling of play and playful teaching within teacher education program

The host galaxies of radio-quiet quasars at 0.5<z<1.0

We present near-infrared H-band imaging of 15 intermediate redshift (0.5<z<1) radio quiet quasars (RQQ) in order to characterize the properties of their host galaxies. We are able to clearly detect the surrounding nebulosity in 12 objects, whereas the object remains unresolved in three cases. For all the resolved objects, we find that the host galaxy is well represented by a de Vaucouleurs r^{1/4} surface brightness law. This is the first reasonably sized sample of intermediate redshift RQQs studied in the near-infrared. The RQQ host galaxies are luminous (average M_H=-26.3+-0.6) and large giant elliptical galaxies (average bulge scale length R_e = 11.3\pm5.8 kpc). RQQ hosts are about 1 mag brighter than the typical low redshift galaxy luminosity L^*, and their sizes are similar to those of galaxies hosting lower redshift RQQs, indicating that there is no significant evolution at least up to z=1 of the host galaxy structure. We also find that RQQ hosts are about 0.5-1 mag fainter than radio-loud quasars (RLQ) hosts at the similar redshift range. The comparison of the host luminosity of intermediate redshift RQQ hosts with that for lower z sources shows a trend that is consistent with that expected from the passive evolution of the stars in the host galaxies. The nuclear luminosity and the nucleus/host galaxy luminosity ratio of the objects in our sample are intermediate between those of lower redshift RQQs and those of higher redshift (z>1) RQQs.Comment: A&A, accepted, 15 pages, 6 figures. Fig. 2 will only be published in the electronic version of A&

The luminous host galaxies of high redshift BL Lac objects

(abridged) We present near-infrared Ks-band imaging of 13 high redshift (0.6 < z < 1.3) BL Lac objects. We clearly detect the host in eight objects, and marginally in three others. In all cases, the host galaxy is well represented by an r^1/4 surface brightness law. The host galaxies of high redshift BL Lacs are large ( ~7 kpc) and very luminous ( = -27.9+-0.7), ~3 mag brighter than L*, and ~1 mag brighter than brightest cluster galaxies. They are also ~1 mag brighter than low redshift radio galaxies and appear to deviate from their K-z relationship. On the other hand, the high luminosities agree with the few optical studies of high redshift BL Lac hosts. The nuclear luminosity and the nucleus-galaxy luminosity ratio of the high redshift BL Lacs are much larger than those in low redshift BL Lacs. This may be due to either a higher intrinsic nuclear luminosity, or enhanced luminosity because of strong beaming. Contrary to what is observed in low redshift BL Lacs, the luminosities of the host galaxy and of the nucleus are fairly well correlated, as expected from the black hole mass - bulge luminosity relationship. High redshift BL Lacs radiate with a wide range of power with respect to their Eddington luminosity, and this power is intermediate between those in nearby BL Lacs and in luminous radio-loud quasars. The high redshift BL Lac host galaxies appear to be ~2 mag brighter than those at low redshift. This is likely due to a strong selection effect in the surveys of BL Lacs that makes observable only the most luminous sources at z > 0.5 and produces a correlation between the nuclear and the host luminosity. However, this may also suggest strong luminosity evolution which is inconsistent with a simple passive evolution of the host galaxies, and requires a contribution from relatively recent star formation episodes.Comment: A&A, accepted, 17 pages, 7 figures. The Appendix will only be published in the electronic version of A&

Genome-Wide Identification of Alternative Splice Forms Down-Regulated by Nonsense-Mediated mRNA Decay in Drosophila

Alternative mRNA splicing adds a layer of regulation to the expression of thousands of genes in Drosophila melanogaster. Not all alternative splicing results in functional protein; it can also yield mRNA isoforms with premature stop codons that are degraded by the nonsense-mediated mRNA decay (NMD) pathway. This coupling of alternative splicing and NMD provides a mechanism for gene regulation that is highly conserved in mammals. NMD is also active in Drosophila, but its effect on the repertoire of alternative splice forms has been unknown, as has the mechanism by which it recognizes targets. Here, we have employed a custom splicing-sensitive microarray to globally measure the effect of alternative mRNA processing and NMD on Drosophila gene expression. We have developed a new algorithm to infer the expression change of each mRNA isoform of a gene based on the microarray measurements. This method is of general utility for interpreting splicing-sensitive microarrays and high-throughput sequence data. Using this approach, we have identified a high-confidence set of 45 genes where NMD has a differential effect on distinct alternative isoforms, including numerous RNA–binding and ribosomal proteins. Coupled alternative splicing and NMD decrease expression of these genes, which may in turn have a downstream effect on expression of other genes. The NMD–affected genes are enriched for roles in translation and mitosis, perhaps underlying the previously observed role of NMD factors in cell cycle progression. Our results have general implications for understanding the NMD mechanism in fly. Most notably, we found that the NMD–target mRNAs had significantly longer 3′ untranslated regions (UTRs) than the nontarget isoforms of the same genes, supporting a role for 3′ UTR length in the recognition of NMD targets in fly

Cattle Mammary Bioreactor Generated by a Novel Procedure of Transgenic Cloning for Large-Scale Production of Functional Human Lactoferrin

Large-scale production of biopharmaceuticals by current bioreactor techniques is limited by low transgenic efficiency and low expression of foreign proteins. In general, a bacterial artificial chromosome (BAC) harboring most regulatory elements is capable of overcoming the limitations, but transferring BAC into donor cells is difficult. We describe here the use of cattle mammary bioreactor to produce functional recombinant human lactoferrin (rhLF) by a novel procedure of transgenic cloning, which employs microinjection to generate transgenic somatic cells as donor cells. Bovine fibroblast cells were co-microinjected for the first time with a 150-kb BAC carrying the human lactoferrin gene and a marker gene. The resulting transfection efficiency of up to 15.79×10−2 percent was notably higher than that of electroporation and lipofection. Following somatic cell nuclear transfer, we obtained two transgenic cows that secreted rhLF at high levels, 2.5 g/l and 3.4 g/l, respectively. The rhLF had a similar pattern of glycosylation and proteolytic susceptibility as the natural human counterpart. Biochemical analysis revealed that the iron-binding and releasing properties of rhLF were identical to that of native hLF. Importantly, an antibacterial experiment further demonstrated that rhLF was functional. Our results indicate that co-microinjection with a BAC and a marker gene into donor cells for somatic cell cloning indeed improves transgenic efficiency. Moreover, the cattle mammary bioreactors generated with this novel procedure produce functional rhLF on an industrial scale