Readiness of students of the magistracy for innovative pedagogical activity

In article questions of readiness of students of a magistracy for innovative pedagogical activity revealРаскрываются вопросы готовности студентов магистратуры к инновационной педагогической деятельност

Assessment of epidemiological and epizootiological situation on tularemia in the Republic of tatarstan

Objective of the study was to assess epidemiological and epizootiological activity of natural tularemia foci in the Republic of Tatarstan for further forecasting of the developments and working out the tactics for implementation of preventive and anti-epidemic measures. Materials and methods. We utilized statistical data, reports of the Center of Hygiene and Epidemiology in the Republic of Tatarstan, and literature sources for the analysis. Results and conclusions. Since 1995, the incidence among the population in Tatarstan has not been registered. Natural tularemia foci are situated in 19 enzootic as regards the infection administrative districts in the territory of the Republic. Epizootiological monitoring of the natural foci suggests that over the last four years their activation occurred. In this contest realization of complex anti-epidemic  measures,  regulated  by  the  current  normative-methodological  documents  remains  relevant,  as  well  as timely and effective diagnostics, including among the patients with a similar to tularemia symptoms

Anthrax in the Republic of Tatarstan (1920–2020)

The aim of the work was to characterize the epidemiological and epizootic situation on anthrax among population and animals in the Republic of Tatarstan over a period of 1920–2020.Materials and methods. The analysis of the epidemiological and epizootic situation is based on the archival data, epidemiological maps of anthrax patients, results of epizootiological-epidemiological survey of anthrax foci conducted by the Rospotrebnadzor Administration in the Republic of Tatarstan and Center of Hygiene and Epidemiology in the Republic of Tatarstan, materials of the Main Directorate of Veterinary Medicine of the Republic of Tatarstan. Microbiological studies of samples from patients and environmental objects were performed in accordance with the requirements of MR 4.2.2413-08 “Laboratory diagnostics and detection of anthrax pathogen”, real-time PCR was set using the AmpliSense Bacillus anthracis-FRT test-system (Central Research Institute of Epidemiology, Moscow). Statistical data processing was carried out using the quantile ranking method.Results and discussion. There are more than 1000 anthrax soil foci in the Republic of Tatarstan, which territorially belongs to the Volga Federal District. Analysis of the epizootic and epidemiological situation in the Republic of Tatarstan over the period of 1920–2020 has revealed that it has undergone significant changes, from mass diseases in animals and humans in early 20th century to sporadic cases of infection among population and animals at the beginning of the 21st century, primarily due to preventive veterinarysanitary measures, including veterinary and sanitary examination of animal products, mass specific immunization of animals against anthrax, arrangement of anthrax cattle burial grounds. In view of the improvement of epizootiological situation and implementation of preventive measures, there was a decrease in the incidence of anthrax among the population. The regions of the Republic have been ranked by the number of animal anthrax cases

THE PRESENT STATE OF THE PROBLEM OF RICKETTSIOSES IN RUSSIA AND NEW APPROACHES TO THE CLASSIFICATION OF DISEASES CAUSED BY SPOTTED FEVER GROUP RICKETTSIAE

The paper presents analysis of the development and the current status of the doctrine about rickettsia and rickettsiosis. Progress in the study of Rickettsia is associated with, improved methods for their detection and isolation - using live biotechnological systems (cultivation in the experimental lines of ticks-sensitive lines of eukaryotic cells) in combination with methods of genotyping and. classical ricketsial methods. The development and. use of new methodological approaches to the study of tick-borne rickettsiae and other alpha-proteobacteria led to an important revision of ideas about genetic diversity, heterogeneity of antigenic and. biological characteristics, ecology and. contribution to the infectious pathology of various representatives of the order Rickettsiales in Russia

Analysis of the Epidemiological Situation of Hemorrhagic Fever with Renal Syndrome in the Russian Federation in 2022 and Forecast of its Development for 2023

The paper provides the analysis of epidemiological process of hemorrhagic fever with renal syndrome (HFRS) in the Russian Federation in the context of federal districts in 2022 and a forecast of the HFRS incidence for 2023. According to the results of the analysis, there was a three-fold increase in the morbidity rates of HFRS in Russia in 2022 as compared to the indicators of 2021. The evidence of epizootiological survey and laboratory studies in certain federal districts of the Russian Federation indicate the continuing tense epidemiological situation on HFRS. In a number of regions of the country, high risk of infection with HFRS is predicted due to the favorable natural and climatic conditions of the winter period 2022/2023 for reservoir hosts of pathogenic for humans Hantaviruses. The findings of infected rodents attest to a high probability of complication of the epidemiological situation in the territories of increased epidemic hazard as regards HFRS

Overview of Hantavirus Infections in the World, the Epidemiological Situation on Hemorrhagic Fever with Renal Syndrome in the Russian Federation in 2021, and Forecast for 2022

The paper presents a description of the epidemiological situation on Hantavirus infection incidence in the countries around the world. Comparative analysis of the intensity and dynamics of the epidemiological process of hemorrhagic fever with renal syndrome (HFRS) in the Russian Federation by Federal Districts in 2021 has been carried out and forecast of the HFRS incidence for 2022 prepared. The study has revealed that tense situation on incidence of hantavirus diseases was observed in the world in 2021. On the territory of the Russian Federation, there was a decrease in the HFRS incidence in 2021 (by 1.7 times compared to 2020). However, the results of epidemiological analysis of the HFRS incidence, epizootiological data and laboratory studies in certain Federal Districts of the Russian Federation indicate that the epidemiological situation on HFRS remains tense. High risk of HFRS infection is predicted in a number of regions of the country due to the favorable natural and climatic conditions in the winter period of 2021–2022 with a heavy snow cover, which contributed to the under-snow reproduction of small mammals, the main carriers of HFRS. The presence of infected rodents testifies to a high likelihood of complication of the epidemiological situation in areas of increased epidemic risk of HFRS

Application of Molecular Typing Methods for Analysis of Strains of Rickettsiae of the Spotted Fever Group and Rabies Virus

50 strains of spotted fever group (SFG) rickettsiae and 36 lyssaviruses strains from the collection of Omsk Research Institute of Natural Focus Infections were identified and typed using the approaches of molecular biology, epidemiology and bioinformatics. The taxonomic status of the studied cultures of SFG rickettsiae was identified. Zonation of the Russian Federation territories according to the spread of SFG pathogenic rickettsiae in their hosts - ixodic ticks was carried out. Lyssaviruses distribution in the territory of Siberia was considered

Review of Hantavirus Infections in the World, Epidemiological Situation on Hemorrhagic Fever with Renal Syndrome in the Russian Federation in 2020 and a Forecast for 2021

The review used the data from operational monitoring carried out by the Reference Center for Monitoring over HFRS – “Kazan Research Institute of Epidemiology and Microbiology of the Rospotrebnadzor”, based on official data provided by the Rospotrebnadzor institutions in the constituent entities of the Russian Federation. Statistical processing was conducted using conventional methods of variation statistics applying the Excel program. Over the past decades, hantavirus diseases have become very relevant and spread throughout the world. In the territory of the Russian Federation, natural foci of HFRS are located in the European part of the country, Western Siberia and Far East. The most epidemically active foci are situated in the European part of Russia. Over the past decade, the intensive incidence rate of HFRS in the Russian Federation stayed within the range of 3.0–9.5 per 100 thousand of the population, the long-term average annual indicator – 5.2 per 100 thousand of the population. In 2020, 3845 cases of HFRS were registered (2.62 per 100,000 of the population). There was a decrease in the incidence of HFRS by 3.6 times, compared with the indicators of 2019. A factor that may have influenced the decrease in the incidence of HFRS was the depression of the epizootic process among small mammals, the main carriers of HFRS pathogens, due to natural and climatic factors. The nature of the distribution of HFRS incidence across the territory of the Russian Federation in 2020 was heterogeneous. Statistical processing of the data made it possible to identify 5 groups of territories that differ in the level of HFRS incidence. Almost all constituent entities of the Volga Federal District and the Kostroma Region belonging to the Central Federal District were classified as groups of territories with high and very high incidence rates. In 2021, the deterioration of the epidemiological situation is predicted in the summer-autumn period of the year in the Volga Federal District and four entities of the Central Federal District

Attachment of HeLa cells during early G1 phase

Both growth factor directed and integrin dependent signal transduction were shown to take place directly after completion of mitosis. The local activation of these signal transduction cascades was investigated in early G1 cells. Interestingly, various key signal transduction proteins were found in blebs at the cell membrane within 30 min after mitosis. These membrane blebs appeared in round, mitotic-like cells and disappeared rapidly during spreading of the cells in G1 phase. In addition to tyrosine-phosphorylated proteins, the blebs contained also phosphorylated FAK and phosphorylated MAP kinase. The formation of membrane blebs in round, mitotic cells before cell spreading is not specific for mitotic cells, because similar features were observed in trypsinized cells. Just before cell spreading also these cells exhibited membrane blebs containing active signal transduction proteins. Inhibition of signal transduction did not affect membrane bleb formation, suggesting that the membrane blebs were formed independent of signal transduction