Proteomics data of two 34kDa bands from ASF and conventional mice

Proteomics data of two 34kDa bands from ASF and conventional mice 2 samples: ASF-34KD; and WT-34K

The Energetics of Molecular Gas in NGC 891 from H_2 and Far-infrared Spectroscopy

We have studied the molecular hydrogen energetics of the edge-on spiral galaxy NGC 891, using a 34 position map in the lowest three pure rotational H_2 lines observed with the Spitzer Infrared Spectrograph. The S(0), S(1), and S(2) lines are bright with an extinction-corrected total luminosity of ~2.8 × 10^7 L_☉, or 0.09% of the total-infrared luminosity of NGC 891. The H_2 line ratios are nearly constant along the plane of the galaxy—we do not observe the previously reported strong drop-off in the S(1)/S(0) line intensity ratio in the outer regions of the galaxy, so we find no evidence for the very massive cold CO-free molecular clouds invoked to explain the past observations. The H_2 level excitation temperatures increase monotonically indicating that there is more than one component to the emitting gas. More than 99% of the mass is in the lowest excitation (T_(ex) ~ 125 K) "warm" component. In the inner galaxy, the warm H_2 emitting gas is ~16% of the CO(1-0)-traced cool molecular gas, while in the outer regions the fraction is twice as high. This large mass of warm gas is heated by a combination of the far-UV photons from stars in photodissociation regions (PDRs) and the dissipation of turbulent kinetic energy. Including the observed far-infrared [O I] and [C II] fine-structure line emission and far-infrared continuum emission in a self-consistent manner to constrain the PDR models, we find essentially all of the S(0) and most (70%) of the S(1) line arise from low excitation PDRs, while most (80%) of the S(2) and the remainder of the S(1) line emission arise from low-velocity microturbulent dissipation

Patterns of MicroRNA Expression in Normal and Early Alzheimer\u27s Disease Human Temporal Cortex: White Matter versus Gray Matter

MicroRNA (miRNA) expression was assessed in human cerebral cortical gray matter (GM) and white matter (WM) in order to provide the first insights into the difference between GM and WM miRNA repertoires across a range of Alzheimer\u27s disease (AD) pathology. RNA was isolated separately from GM and WM portions of superior and middle temporal cerebral cortex (N = 10 elderly females, postmortem interval \u3c 4 h). miRNA profiling experiments were performed using state-of-the-art Exiqon© LNA-microarrays. A subset of miRNAs that appeared to be strongly expressed according to the microarrays did not appear to be conventional miRNAs according to Northern blot analyses. Some well-characterized miRNAs were substantially enriched in WM as expected. However, most of the miRNA expression variability that correlated with the presence of early AD-related pathology was seen in GM. We confirm that downregulation of a set of miRNAs in GM (including several miR-15/107 genes and miR-29 paralogs) correlated strongly with the density of diffuse amyloid plaques detected in adjacent tissue. A few miRNAs were differentially expressed in WM, including miR-212 that is downregulated in AD and miR-424 which is upregulated in AD. The expression of certain miRNAs correlates with other miRNAs across different cases, and particular subsets of miRNAs are coordinately expressed in relation to AD-related pathology. These data support the hypothesis that patterns of miRNA expression in cortical GM may contribute to AD pathogenetically, because the aggregate change in miRNA expression observed early in the disease would be predicted to cause profound changes in gene expression

Anti-Argonaute RIP-Chip Shows that miRNA Transfections Alter Global Patterns of mRNA Recruitment to Microribonucleoprotein Complexes

MicroRNAs (miRNAs) play key roles in gene expression regulation by guiding Argonaute (AGO)-containing microribonucleoprotein (miRNP) effector complexes to target polynucleotides. There are still uncertainties about how miRNAs interact with mRNAs. Here we employed a biochemical approach to isolate AGO-containing miRNPs from human H4 tumor cells by co-immunoprecipitation (co-IP) with a previously described anti-AGO antibody. Co-immunoprecipitated (co-IPed) RNAs were subjected to downstream Affymetrix Human Gene 1.0 ST microarray analysis. During rigorous validation, the RIP-Chip assay identified target mRNAs specifically associated with AGO complexes. RIP-Chip was performed after transfecting brain-enriched miRNAs (miR-107, miR-124, miR-128, and miR-320) and nonphysiologic control miRNA to identify miRNA targets. As expected, the miRNA transfections altered the mRNA content of the miRNPs. Specific mRNA species recruited to miRNPs after miRNA transfections were moderately in agreement with computational target predictions. In addition to recruiting mRNA targets into miRNPs, miR-107 and to a lesser extent miR-128, but not miR-124 or miR-320, caused apparent exclusion of some mRNAs that are normally associated with miRNPs. MiR-107 and miR-128 transfections also result in decreased AGO mRNA and protein levels. However, AGO mRNAs were not recruited to miRNPs after either miR-107 or miR-128 transfection, confirming that miRNAs may alter gene expression without stable association between particular mRNAs and miRNPs. In summary, RIP-Chip assays constitute an optimized, validated, direct, and high-throughput biochemical assay that provides data about specific miRNA:mRNA interactions, as well as global patterns of regulation by miRNAs

The Energetics of Molecular Gas in NGC 891 from H2 and Far-infrared Spectroscopy

We have studied the molecular hydrogen energetics of the edge-on spiral galaxy NGC?891, using a 34 position map in the lowest three pure rotational H2 lines observed with the Spitzer Infrared Spectrograph. The S(0), S(1), and S(2) lines are bright with an extinction-corrected total luminosity of ~2.8 × 107 L ?, or 0.09% of the total-infrared luminosity of NGC?891. The H2 line ratios are nearly constant along the plane of the galaxy?we do not observe the previously reported strong drop-off in the S(1)/S(0) line intensity ratio in the outer regions of the galaxy, so we find no evidence for the very massive cold CO-free molecular clouds invoked to explain the past observations. The H2 level excitation temperatures increase monotonically indicating that there is more than one component to the emitting gas. More than 99% of the mass is in the lowest excitation (T ex ~ 125 K) warm component. In the inner galaxy, the warm H2 emitting gas is ~16% of the CO(1-0)-traced cool molecular gas, while in the outer regions the fraction is twice as high. This large mass of warm gas is heated by a combination of the far-UV photons from stars in photodissociation regions (PDRs) and the dissipation of turbulent kinetic energy. Including the observed far-infrared [O I] and [C II] fine-structure line emission and far-infrared continuum emission in a self-consistent manner to constrain the PDR models, we find essentially all of theS(0) and most (70%) of the S(1) line arise from low excitation PDRs, while most (80%) of the S(2) and the remainder of the S(1) line emission arise from low-velocity microturbulent dissipation

Individual MicroRNAs (miRNAs) Display Distinct mRNA Targeting Rules

MicroRNAs (miRNAs) guide Argonaute (AGO)-containing microribonucleoprotein (miRNP) complexes to target mRNAs.It has been assumed that miRNAs behave similarly to each other with regard to mRNA target recognition. The usual assumptions, which are based on prior studies, are that miRNAs target preferentially sequences in the 3\u27UTR of mRNAs,guided by the 5\u27 seed portion of the miRNAs. Here we isolated AGO- and miRNA-containing miRNPs from human H4 tumor cells by co-immunoprecipitation (co-IP) with anti-AGO antibody. Cells were transfected with miR-107, miR-124,miR-128, miR-320, or a negative control miRNA. Co-IPed RNAs were subjected to downstream high-density Affymetrix Human Gene 1.0 ST microarray analyses using an assay we validated previously-a RIP-Chip experimental design. RIP-Chip data provided a list of mRNAs recruited into the AGO-miRNP in correlation to each miRNA. These experimentally identified miRNA targets were analyzed for complementary six nucleotide seed sequences within the transfected miRNAs. We found that miR-124 targets tended to have sequences in the 3\u27UTR that would be recognized by the 5\u27 seed of miR-124, as described in previous studies. By contrast, miR-107 targets tended to have \u27seed\u27 sequences in the mRNA open reading frame, but not the 3\u27 UTR. Further, mRNA targets of miR-128 and miR-320 are less enriched for 6-mer seed sequences in comparison to miR-107 and miR-124. In sum, our data support the importance of the 5\u27 seed in determining binding characteristics for some miRNAs; however, the binding rules are complex, and individual miRNAs can have distinct sequence determinants that lead to mRNA targeting

The Energetics of Molecular Gas in NGC 891 from H2 and FIR Spectroscopy

We have studied the molecular hydrogen energetics of the edge-on spiral galaxy NGC\,891, using a 34-position map in the lowest three pure rotational H$_2$ lines observed with the Spitzer Infrared Spectrograph. The S(0), S(1), and S(2) lines are bright with an extinction corrected total luminosity of $\sim2.8 \times 10^{7}$ L$_{\odot}$, or 0.09\% of the total-infrared luminosity of NGC\,891. The H$_2$ line ratios are nearly constant along the plane of the galaxy -- we do not observe the previously reported strong drop-off in the S(1)/S(0) line intensity ratio in the outer regions of the galaxy, so we find no evidence for the very massive cold CO-free molecular clouds invoked to explain the past observations. The H$_2$ level excitation temperatures increase monotonically indicating more than one component to the emitting gas. More than 99\% of the mass is in the lowest excitation (T$_{ex}$ $\sim$125 K) ``warm'' component. In the inner galaxy, the warm H$_2$ emitting gas is $\sim$15\% of the CO(1-0)-traced cool molecular gas, while in the outer regions the fraction is twice as high. This large mass of warm gas is heated by a combination of the far-UV photons from stars in photo-dissociation regions (PDRs) and the dissipation of turbulent kinetic energy. Including the observed far-infrared [OI] and [CII] fine-structure line emission and far-infrared continuum emission in a self-consistent manner to constrain the PDR models, we find essentially all of the S(0) and most (70\%) of the S(1) line arises from low excitation PDRs, while most (80\%) of the S(2) and the remainder of the S(1) line emission arises from low velocity microturbulent dissipation.Comment: Accepted for publication in The Astrophysical Journal. Figure 10 available at http://www.physics.uoc.gr/~vassilis/papers/ngc891.pd

Gender differences in the development of uremic cardiomyopathy following partial nephrectomy: Role of progesterone

Gender difference has been suggested as a risk factor for developing cardiovascular and renal diseases in humans and experimental animals. As a major sex hormone, progesterone was reported to compete with cardiotonic steroid binding to Na/K-ATPase. Our previous publication demonstrated that cardiotonic steroids (e.g., marinobufagenin) play an important role in the development of experimental uremic cardiomyopathy. We also observed that the putative mineralocorticoid antagonists, spironolactone and its major metabolite canrenone, antagonize binding of cardiotonic steroids to Na/K-ATPase in a competitive manner and also ameliorate experimental uremic cardiomyopathy induced by partial nephrectomy. In the following studies, we noted that progesterone displayed competitive inhibition of cardiotonic steroid binding to Na/K-ATPase and partially inhibited collagen synthesis induced by marinobufagenin in cultured cardiac fibroblasts. Therefore, we sought to examine whether female rats displayed less uremic cardiomyopathy than male rats when subjected to partial nephrectomy. Although partial nephrectomy caused the induction of smaller increases in blood pressure of female rats, they appeared to be similarly susceptible to cardiac remodeling induced by partial nephrectomy in terms of hypertrophy and fibrosis as age-matched male rats. The possible explanations for our findings are therefore discussed

Enterococcus faecalis Immunoregulates Osteoclastogenesis of Macrophages

published_or_final_versio

Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models

Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome. In contrast, how dietary fiber impacts type 1 diabetes is less clear