Life on the ice-edge: Paleoenvironmental significance of the radiolarian species Amphimelissa setosa in the northern hemisphere

The high-latitude Northern Hemisphere is a key region in the global climate balance. Variations in sea-ice extent affect biological productivity, CO2 exchange and carbon drawdown. Marine proxies indicative of proximity of the ice-marginal zone are therefore essential to understand these processes. Amphimelissa setosa is nowadays a dominant radiolarian species in the Arctic basin and very abundant in the high-latitude North Atlantic. This species, now absent from the North Pacific, has been widely used as a qualitative proxy of modern and past environmental conditions in the high-latitude Northern Hemisphere. Using our new and published data on the distribution of A. setosa in plankton, sediment trap, surface sediment and downcore samples, we provide a quantitative ecological context for the occurrence of this species. We find that the optimal depth and season of A. setosa in the modern North Atlantic and the Chukchi Sea are 160 m and the late boreal summer/early fall (August–October), respectively. A regression model combining environmental variables (temperature, salinity, silicate and chlorophyll-a concentrations, apparent oxygen utilization, sea-ice) at that season and depth, are able to explain 43% of the distribution of this species in surface sediments. Based on these new findings, we conclude that the presence of A. setosa in surface sediments is closely related to high primary production in the proximity of the sea-ice and areas of ice rafting. The onset of this species started at ca. 1.5 Ma in the North Pacific, linked to a gradual cooling, increased silica availability and southward advance of the ice-margins since the Early Pleistocene. Amphimelissa setosa’s decline in this region was likely caused by the development of a quasi-permanent halocline, perennial sea-ice and depletion of silica during marine isotope stage 4. In the high-latitude North Atlantic, the relative abundance of A. setosa appears to be related to cooling and supply of dissolved silica from the continent during ice-rafting events. The comprehensive approach taken in this study suggest that A. setosa is a useful proxy to explore past variations in the ice-cover in the high-latitude Northern Hemisphere.Shirshov Institute of Oceanology | Ref. 0149-2019-000

229Th the Bridge Between Nuclear and Atomic Interactions

The precise measurement of time has been a goal of physicists for centuries. With every new increase in our ability to measure time we have discovered new phenomena. The most advanced clocks available to us currently are atomic clocks that use electronic transitions to track the passage of time. In this proposal, I put forward the framework for the first nuclear clock estimated to be 1000 to 10000 times more precise than the current atomic clocks. This research will explore in detail the atomic nuclear interactions and help perfect and refine current atomic-nuclear interaction models. The realization of a {sup 229}Th nuclear clock will allow tests of cosmology by measuring the change of the fine structure constant as a function of time. The results of these experiments could dramatically alter our view of the universe, its past and future evolution. Precision clocks - with fundamental physics applications - require a long-lived quantum transition (two-level system) that is immune to external perturbations. Nuclear transitions would be better suited than atomic transitions for these applications except that nuclear transitions are typically much higher in energy and therefore cannot be accessed with table-top lasers. There is, however, one promising nuclear transition: the doublet between the ground and first excited states of the {sup 229}Th nucleus discovered by Helmer and Reich. This doublet has an energy splitting of 7.6 {+-} 0.5 eV, a spin difference of 1 h-bar, and an excited state half-life that could be as long as hours. A precision clock based on the {sup 229}Th nuclear doublet has been proposed by Peik et al. Their design is similar to the ion clock research being conducted at NIST in Boulder, CO. However, the NIST researchers use atomic transitions for their frequency standards. In the {sup 229}Th nuclear doublet transition is the frequency standard while atomic transitions are used to cool the ions and for probing the state of the {sup 229}Th nucleus. Recently, Campbell et al. have trapped and cooled {sup 232}Th{sup 3+} at Georgia Institute of Technology. This is a large step forward in the realization of a nuclear clock. The Georgia Tech group is already a collaborator on this project and we are in discussions with the NIST Boulder group about collaboration. In order to determine the suitability of the {sup 229}Th nuclear doublet for a precision clock, the half-life of the excited-state needs to be measured. Current estimates of the half-life vary from 10 {micro}s to 1000 hours. The longer the half-life, the narrower the natural linewidth of the state and the more desirable the transition is for potential applications. In this proposal, I outline the necessary research to be conducted to determine the half-life and exact wavelength of the nuclear doublet transition in {sup 229}Th. This research will lead to a deeper understanding of atomic-nuclear interactions important for our knowledge of high energy density science. It will provide a spectroscopy measurement of the lowest known nuclear transition ever and open the doorway for the development of a nuclear clock with unprecedented precision

229Th the Bridge Between Nuclear and Atomic Interactions

The precise measurement of time has been a goal of physicists for centuries. With every new increase in our ability to measure time we have discovered new phenomena. The most advanced clocks available to us currently are atomic clocks that use electronic transitions to track the passage of time. In this proposal, I put forward the framework for the first nuclear clock estimated to be 1000 to 10000 times more precise than the current atomic clocks. This research will explore in detail the atomic nuclear interactions and help perfect and refine current atomic-nuclear interaction models. The realization of a {sup 229}Th nuclear clock will allow tests of cosmology by measuring the change of the fine structure constant as a function of time. The results of these experiments could dramatically alter our view of the universe, its past and future evolution. Precision clocks - with fundamental physics applications - require a long-lived quantum transition (two-level system) that is immune to external perturbations. Nuclear transitions would be better suited than atomic transitions for these applications except that nuclear transitions are typically much higher in energy and therefore cannot be accessed with table-top lasers. There is, however, one promising nuclear transition: the doublet between the ground and first excited states of the {sup 229}Th nucleus discovered by Helmer and Reich. This doublet has an energy splitting of 7.6 {+-} 0.5 eV, a spin difference of 1 h-bar, and an excited state half-life that could be as long as hours. A precision clock based on the {sup 229}Th nuclear doublet has been proposed by Peik et al. Their design is similar to the ion clock research being conducted at NIST in Boulder, CO. However, the NIST researchers use atomic transitions for their frequency standards. In the {sup 229}Th nuclear doublet transition is the frequency standard while atomic transitions are used to cool the ions and for probing the state of the {sup 229}Th nucleus. Recently, Campbell et al. have trapped and cooled {sup 232}Th{sup 3+} at Georgia Institute of Technology. This is a large step forward in the realization of a nuclear clock. The Georgia Tech group is already a collaborator on this project and we are in discussions with the NIST Boulder group about collaboration. In order to determine the suitability of the {sup 229}Th nuclear doublet for a precision clock, the half-life of the excited-state needs to be measured. Current estimates of the half-life vary from 10 {micro}s to 1000 hours. The longer the half-life, the narrower the natural linewidth of the state and the more desirable the transition is for potential applications. In this proposal, I outline the necessary research to be conducted to determine the half-life and exact wavelength of the nuclear doublet transition in {sup 229}Th. This research will lead to a deeper understanding of atomic-nuclear interactions important for our knowledge of high energy density science. It will provide a spectroscopy measurement of the lowest known nuclear transition ever and open the doorway for the development of a nuclear clock with unprecedented precision

Predator-Induced Vertical Behavior of a Ctenophore

Although many studies have focused on Mnemiopsis leidyi predation, little is known about the role of this ctenophore as prey when abundant in native and invaded pelagic systems. We examined the response of the ctenophore M. leidyi to the predatory ctenophore Beroe ovata in an experiment in which the two species could potentially sense each other while being physically separated. On average, M. leidyi responded to the predator’s presence by increasing variability in swimming speeds and by lowering their vertical distribution. Such behavior may help explain field records of vertical migration, as well as stratified and near-bottom distributions of M. leidyi

Genetic variation exists for telomeric array organization within and among the genomes of normal, immortalized, and transformed chicken systems

This study investigated telomeric array organization of diverse chicken genotypes utilizing in vivo and in vitro cells having phenotypes with different proliferation potencies. Our experimental objective was to characterize the extent and nature of array variation present to explore the hypothesis that mega-telomeres are a universal and fixed feature of chicken genotypes. Four different genotypes were studied including normal (UCD 001, USDA-ADOL Line 0), immortalized (DF-1), and transformed (DT40) cells. Both cytogenetic and molecular approaches were utilized to develop an integrated view of telomeric array organization. It was determined that significant variation exists within and among chicken genotypes for chromosome-specific telomeric array organization and total genomic-telomeric sequence content. Although there was variation for mega-telomere number and distribution, two mega-telomere loci were in common among chicken genetic lines (GGA 9 and GGA W). The DF-1 cell line was discovered to maintain a complex derivative karyotype involving chromosome fusions in the homozygous and heterozygous condition. Also, the DF-1 cell line was found to contain the greatest amount of telomeric sequence per genome (17%) as compared to UCD 001 (5%) and DT40 (1.2%). The chicken is an excellent model for studying unique and universal features of vertebrate telomere biology, and characterization of the telomere length variation among genotypes will be useful in the exploration of mechanisms controlling telomere length maintenance in different cell types having unique phenotypes

Surrogate Measurement of the \u3csup\u3e238\u3c/sup\u3ePu(\u3cem\u3en,f\u3c/em\u3e\u3c/em\u3e) Cross Section

The neutron-induced fission cross section of 238Pu was determined using the surrogate ratio method. The (n,f) cross section over an equivalent neutron energy range 5–20 MeV was deduced from inelastic α-induced fission reactions on 239Pu, with 235U(α,α′f) and 236U(α,α′f) used as references. These reference reactions reflect 234U(n,f) and 235U(n,f) yields, respectively. The deduced 238Pu(n,f) cross section agrees well with standard data libraries up to ~10 MeV, although larger values are seen at higher energies. The difference at higher energies is less than 20%

Inheritance of Telomere Length in a Bird

Telomere dynamics are intensively studied in human ageing research and epidemiology, with many correlations reported between telomere length and age-related diseases, cancer and death. While telomere length is influenced by environmental factors there is also good evidence for a strong heritable component. In human, the mode of telomere length inheritance appears to be paternal and telomere length differs between sexes, with females having longer telomeres than males. Genetic factors, e.g. sex chromosomal inactivation, and non-genetic factors, e.g. antioxidant properties of oestrogen, have been suggested as possible explanations for these sex-specific telomere inheritance and telomere length differences. To test the influence of sex chromosomes on telomere length, we investigated inheritance and sex-specificity of telomere length in a bird species, the kakapo (Strigops habroptilus), in which females are the heterogametic sex (ZW) and males are the homogametic (ZZ) sex. We found that, contrary to findings in humans, telomere length was maternally inherited and also longer in males. These results argue against an effect of sex hormones on telomere length and suggest that factors associated with heterogamy may play a role in telomere inheritance and sex-specific differences in telomere length

On the genetic involvement of apoptosis-related genes in Crohn's disease as revealed by an extended association screen using 245 markers: no evidence for new predisposing factors

Crohn's disease (CD) presents as an inflammatory barrier disease with characteristic destructive processes in the intestinal wall. Although the pathomechanisms of CD are still not exactly understood, there is evidence that, in addition to e.g. bacterial colonisation, genetic predisposition contributes to the development of CD. In order to search for predisposing genetic factors we scrutinised 245 microsatellite markers in a population-based linkage mapping study. These microsatellites cover gene loci the encoded protein of which take part in the regulation of apoptosis and (innate) immune processes. Respective loci contribute to the activation/suppression of apoptosis, are involved in signal transduction and cell cycle regulators or they belong to the tumor necrosis factor superfamily, caspase related genes or the BCL2 family. Furthermore, several cytokines as well as chemokines were included. The approach is based on three steps: analyzing pooled DNAs of patients and controls, verification of significantly differing microsatellite markers by genotyping individual DNA samples and, finally, additional reinvestigation of the respective gene in the region covered by the associated microsatellite by analysing single-nucleotide polymorphisms (SNPs). Using this step-wise process we were unable to demonstrate evidence for genetic predisposition of the chosen apoptosis- and immunity-related genes with respect to susceptibility for CD

Collaborative Action of Brca1 and CtIP in Elimination of Covalent Modifications from Double-Strand Breaks to Facilitate Subsequent Break Repair

Topoisomerase inhibitors such as camptothecin and etoposide are used as anti-cancer drugs and induce double-strand breaks (DSBs) in genomic DNA in cycling cells. These DSBs are often covalently bound with polypeptides at the 3′ and 5′ ends. Such modifications must be eliminated before DSB repair can take place, but it remains elusive which nucleases are involved in this process. Previous studies show that CtIP plays a critical role in the generation of 3′ single-strand overhang at “clean” DSBs, thus initiating homologous recombination (HR)–dependent DSB repair. To analyze the function of CtIP in detail, we conditionally disrupted the CtIP gene in the chicken DT40 cell line. We found that CtIP is essential for cellular proliferation as well as for the formation of 3′ single-strand overhang, similar to what is observed in DT40 cells deficient in the Mre11/Rad50/Nbs1 complex. We also generated DT40 cell line harboring CtIP with an alanine substitution at residue Ser332, which is required for interaction with BRCA1. Although the resulting CtIPS332A/−/− cells exhibited accumulation of RPA and Rad51 upon DNA damage, and were proficient in HR, they showed a marked hypersensitivity to camptothecin and etoposide in comparison with CtIP+/−/− cells. Finally, CtIPS332A/−/−BRCA1−/− and CtIP+/−/−BRCA1−/− showed similar sensitivities to these reagents. Taken together, our data indicate that, in addition to its function in HR, CtIP plays a role in cellular tolerance to topoisomerase inhibitors. We propose that the BRCA1-CtIP complex plays a role in the nuclease-mediated elimination of oligonucleotides covalently bound to polypeptides from DSBs, thereby facilitating subsequent DSB repair

Photoemission analysis of chemically modified TlBr surfaces for improved radiation detectors

Abstract not provide