Development of an improved membrane for a vapor diffusion water recovery process

Recovery of potable water from urine on manned space missions of extended duration was the objective of work aimed at the improvement of membrane performance for the vapor diffusion process (VDR). Kynar, Teflon, PVC, and polysulfone candidate membranes were evaluated from chemical, thermal, mechanical, and fabricating standpoints to determine their suitability for operation in the VDR pervaporation module. Pervaporation rates and other performance characteristics were determined in a breadboard pervaporator test rig. Kynar and Teflon membranes were demonstrated to be chemically stable at pervaporation temperatures in urine pretreated with chromic acid bactericide. The separation of the pervaporator and condenser modules, the use of a recirculating sweep gas to conduct pervaporate to the condenser, and the selection of a hollow fiber membrane configuration for pervaporator module design is recommended as a result of the investigation

Indicators of replicative damage in equine tendon fibroblast monolayers

<p>Background: Superficial digital flexor tendon (SDFT) injuries of horses usually follow cumulative matrix microdamage; it is not known why the reparative abilities of tendon fibroblasts are overwhelmed or subverted. Relevant in vitro studies of this process require fibroblasts not already responding to stresses caused by the cell culture protocols. We investigated indicators of replicative damage in SDFT fibroblast monolayers, effects of this on their reparative ability, and measures that can be taken to reduce it.</p> <p>Results: We found significant evidence of replicative stress, initially observing consistently large numbers of binucleate (BN) cells. A more variable but prominent feature was the presence of numerous gammaH2AX (γH2AX) puncta in nuclei, this being a histone protein that is phosphorylated in response to DNA double-stranded breaks (DSBs). Enrichment for injury detection and cell cycle arrest factors (p53 (ser15) and p21) occurred most frequently in BN cells; however, their numbers did not correlate with DNA damage levels and it is likely that the two processes have different causative mechanisms. Such remarkable levels of injury and binucleation are usually associated with irradiation, or treatment with cytoskeletal-disrupting agents.</p> <p>Both DSBs and BN cells were greatest in subconfluent (replicating) monolayers. The DNA-damaged cells co-expressed the replication markers TPX2/repp86 and centromere protein F. Once damaged in the early stages of culture establishment, fibroblasts continued to express DNA breaks with each replicative cycle. However, significant levels of cell death were not measured, suggesting that DNA repair was occurring. Comet assays showed that DNA repair was delayed in proportion to levels of genotoxic stress.</p> <p>Conclusions: Researchers using tendon fibroblast monolayers should assess their “health” using γH2AX labelling. Continued use of early passage cultures expressing initially high levels of γH2AX puncta should be avoided for mechanistic studies and ex-vivo therapeutic applications, as this will not be resolved with further replicative cycling. Low density cell culture should be avoided as it enriches for both DNA damage and mitotic defects (polyploidy). As monolayers differing only slightly in baseline DNA damage levels showed markedly variable responses to a further injury, studies of effects of various stressors on tendon cells must be very carefully controlled.</p&gt

Investigating Analytical and Numerical Methods to Predict Satellite Orbits Using Two-Line Element Sets

As Low Earth Orbit (LEO) contains an ever-increasing number of objects, the prediction of future object positions must be precise in order to avoid collisions. Object positions are distributed in two-line element (TLE) sets and are generated using the analytical propagator known as Simplified General Perturbations 4 (SGP4). However, a numerical integrator called Special Perturbations (SP) provides an alternative approach to TLE generation and propagation. In this thesis, TLE accuracy was determined with both models, and the length of time that a single element set can provide valid information was also established. With two-line element sets as data, comparisons were made between the numerical integrator and the analytical model for objects in LEO, particularly the Hubble Space Telescope (HST). The results obtained indicated that the propagated position error using SGP4 remained within 1 km of the expected position during a 7 day period from the epoch of the initial element set. Additionally, the majority of comparisons between SGP4 and SP resulted in a higher error while using the numerical integrator, although some comparisons showed a lower propagated position error with SP. This final result shows a potential for SP to provide more accurate position information than SGP4 in future research

A detailed description of the sequential probability ratio test for 2-IMU FDI

The sequential probability ratio test (SPRT) for 2-IMU FDI (inertial measuring unit failure detection/isolation) is described. The SPRT is a statistical technique for detecting and isolating soft IMU failures originally developed for the strapdown inertial reference unit. The flowchart of a subroutine incorporating the 2-IMU SPRT is included

Is therapy based on endoscopy results better than empiric therapy for dyspepsia?

In the initial management of dyspepsia for patients without "alarm" symptoms (weight loss, recurrent vomiting, dysphagia, anemia, evidence of bleeding, onset of dyspepsia after age 45 years), therapy based on the results of early endoscopy was not better than empiric acid suppression (antisecretory therapy) or a Helicobacter pylori "test and treat" strategy in reducing symptoms or improving quality of life (strength of recommendation [SOR]: A, based on a systematic review). Results from studies of patient satisfaction comparing early endoscopy with empiric medication therapy are conflicting (SOR: A, based on 2 randomized controlled trials [RCTs]). Though formal cost analyses are not available, a strategy using "test and treat" as opposed to early endoscopy, results in significantly fewer endoscopies, which when formally evaluated, may translate into a more cost-effective strategy of care (SOR: A, based on a systematic review). Long-term followup suggests that patients receiving "test and treat" therapy may require fewer antisecretory medication prescriptions compared with patients receiving early endoscopy (SOR: B, based on a single RCT)

Project Dawdler: a Proposal in Response to a Low Reynolds Number Station Keeping Mission

In direct response to Request for Proposals: Flight at very low Reynolds numbers - a station keeping mission, the members of Design Squad E present Project Dawdler: a remotely-piloted airplane supported by an independently controlled take-off cart. A brief introduction to Project Dawdler's overall mission and design, is given. The Dawdler is a remotely-piloted airplane designed to fly in an environmentally-controlled closed course at a Reynolds number of 10(exp 5) and at a cruise velocity of 25 ft/s. The two primary goals were to minimize the flight Reynolds number and to maximize the loiter time. With this in mind, the general design of the airplane was guided by the belief that a relatively light aircraft producing a fairly large amount of lift would be the best approach. For this reason the Dawdler utilizes a canard rather than a conventional tail for longitudinal control, primarily because the canard contributes a positive lift component. The Dawdler also has a single vertical tail mounted behind the wing for lateral stability, half of which is used as a rudder for yaw control. Due to the fact that the power required to take-off and climb to altitude is much greater than that required for cruise flight and simple turning maneuvers, it was decided that a take-off cart be used. Based on the current design, there are two unknowns which could possibly threaten the success of Project Dawdler. First, the effect of the fully-movable canard with its large appropriation of total lift on the performance of the plane, and secondly, the ability of the take-off procedure to go as planned are examined. These are questions which can only be answered by a prototype

Stress intensity factors for an infinite plate with radial cracks emanating from an internal hole and subjected to cylindrical bending Progress report

Method for stress intensity factors for infinite plate with radial cracks emanating from internal hole and subjected to cylindrical bendin