Quantitative Analysis of Candida Cell Wall Components by Flow Cytometrywith Triple-Fluorescence Staining

This work was supported by the European Commission within the FP7 Framework Programme [Fungitect-Grant No 602125]. We also thank Thomas Sauer, Vienna Biocenter Campus (VBC), Austria, for technical support at the FACS facility of the MFPL, Karl Kuchler, MFPL-Department of Medical Biochemistry, Medical University of Vienna, Max F. Perutz Laboratories, Campus Vienna Biocenter, Vienna, Austria and Ernst Thuer, Centre for Genomic Regulation, Barcelona, Spain, for advice on statistical approaches. Neil Gow acknowledges the support of the Wellcome Trust and the MRC Centre for Medical MycologyPeer reviewedPublisher PD

On the total curvatures of a tame function

Given a definable function f, enough differentiable, we study the continuity of the total curvature function t --> K(t), total curvature of the level {f=t}, and the total absolute curvature function t-->|K| (t), total absolute curvature of the level {f=t}. We show they admits at most finitely many discontinuities

Computing the local pressure in molecular dynamics simulations

Computer simulations of inhomogeneous soft matter systems often require accurate methods for computing the local pressure. We present a simple derivation, based on the virial relation, of two equivalent expressions for the local (atomistic) pressure in a molecular dynamics simulation. One of these expressions, previously derived by other authors via a different route, involves summation over interactions between particles within the region of interest; the other involves summation over interactions across the boundary of the region of interest. We illustrate our derivation using simulations of a simple osmotic system; both expressions produce accurate results even when the region of interest over which the pressure is measured is very small.Comment: 11 pages, 4 figure

Trace Metals Bioaccumulation Potentials of Three Indigenous Grasses Grown on Polluted Soils Collected Around Mining Areas in Pretoria, South Africa

The rapid increase in the number of industries may have increased the levels of trace metals in the soil. Phytoremediation of these polluted soils using indigenous grasses is now considered an alternative method in remediating these polluted soils. The present study investigated and compared the ability of three indigenous grasses as bioaccumulators of trace metals from polluted soils. Seeds of these grasses were introduced into pots containing polluted soil samples after the addition of organic manure. The seeds of the grasses were allowed to germinate and grow to maturity before harvesting. The harvested grasses were later separated into shoots and roots and the trace metal contents were determined using ICP –MS. From all the grasses, the concentrations of trace metals in the roots were more than those recorded in the shoot with a significant difference (P < 0.05). The transfer factor (TF) showed that Zn was the most bioaccumulated trace metals by all the grasses followed by Pb, Mn, and Cu respectively. Chromium concentration from the shoot of the grasses was in the order Urochlora moasambicensis > Themeda trianda > Cynodon dactylon. The study concluded that the three grasses used were all able to bioaccumulate trace metals in a similar proportion from the polluted soils. However, since livestock feed on these grasses, they should not be allowed to feed on the grasses used in this study especially when harvested from a polluted soil due to their bioaccumulative potentials

Material Modelling of the Photopolymers for Additive Manufacturing Processes

Ultraviolet (UV) curing of polymers is a key phenomenon for several additive manufacturing technologies. This contribution presents a model relating the process parameters of UV light intensity and temperature to the thermal and mechanical properties of the polymer and the experimental results used to calibrate the model. Moreover, photo-differential scanning calorimetry (photo-DSC) measurements are performed to investigate the crosslinking reaction and to model the degree of cure as a function of the light intensity and temperature. The viscoelastic properties are measured by UV rheometry and it is shown that the classical time-cure superposition principle can equally be applied to the experimental results. Complete curing and mechanical model equations are provided to describe the material behavior as a result of our experimental findings.Mechanical Engineerin

Non-classical Photon Statistics For Two-mode Optical Fields

The non-classical property of subpoissonian photon statistics is extended from one to two-mode electromagnetic fields, incorporating the physically motivated property of invariance under passive unitary transformations. Applications to squeezed coherent states, squeezed thermal states, and superposition of coherent states are given. Dependences of extent of non-classical behaviour on the independent squeezing parameters are graphically displayed.Comment: 15 pages, RevTex, 5 figures, available by sending email to [email protected]

N-RAS (neuroblastoma RAS viral oncogene homolog)

Review on N-RAS (neuroblastoma RAS viral oncogene homolog), with data on DNA, on the protein encoded, and where the gene is implicated

KRAS (Kirsten rat sarcoma viral oncogene homolog)

Review on KRAS (Kirsten rat sarcoma viral oncogene homolog), with data on DNA, on the protein encoded, and where the gene is implicated

HRAS (Harvey rat sarcoma viral oncogene homolog)

Review on HRAS (Harvey rat sarcoma viral oncogene homolog), with data on DNA, on the protein encoded, and where the gene is implicated

RAS family

Deep insight on RAS family