44 research outputs found
Effects of Surface Roughness on the Electrochemical Reduction of CO₂ over Cu
We have investigated the role of surface roughening on the CO₂ reduction reaction (CO₂RR) over Cu. The activity and product selectivity of Cu surfaces roughened by plasma pretreatment in Ar, O₂, or N₂ were compared with that of electrochemically polished Cu samples. Differences in total and product current densities, the ratio of current densities for HER (the hydrogen evolution reaction) to CO₂RR, and the ratio of current densities for C₂₊ to C₁ products depend on the electrochemically active surface and are nearly independent of plasma composition. Theoretical analysis of an electropolished and roughened Cu surface reveals a higher fraction of undercoordinated Cu sites on the roughened surface, sites that bind CO preferentially. Roughened surfaces also contain square sites similar to those on a Cu(100) surface but with neighboring step sites, which adsorb OC–COH, a precursor to C₂₊ products. These findings explain the increases in the formation of oxygenates and hydrocarbons relative to CO and the ratio of oxygenates to hydrocarbons observed with increasing surface roughness
Effects of Surface Roughness on the Electrochemical Reduction of CO₂ over Cu
We have investigated the role of surface roughening on the CO₂ reduction reaction (CO₂RR) over Cu. The activity and product selectivity of Cu surfaces roughened by plasma pretreatment in Ar, O₂, or N₂ were compared with that of electrochemically polished Cu samples. Differences in total and product current densities, the ratio of current densities for HER (the hydrogen evolution reaction) to CO₂RR, and the ratio of current densities for C₂₊ to C₁ products depend on the electrochemically active surface and are nearly independent of plasma composition. Theoretical analysis of an electropolished and roughened Cu surface reveals a higher fraction of undercoordinated Cu sites on the roughened surface, sites that bind CO preferentially. Roughened surfaces also contain square sites similar to those on a Cu(100) surface but with neighboring step sites, which adsorb OC–COH, a precursor to C₂₊ products. These findings explain the increases in the formation of oxygenates and hydrocarbons relative to CO and the ratio of oxygenates to hydrocarbons observed with increasing surface roughness
Small RNA interference-mediated gene silencing of heparanase abolishes the invasion, metastasis and angiogenesis of gastric cancer cells
<p>Abstract</p> <p>Background</p> <p>Heparanase facilitates the invasion and metastasis of cancer cells, and is over-expressed in many kinds of malignancies. Our studies indicated that heparanase was frequently expressed in advanced gastric cancers. The aim of this study is to determine whether silencing of heparanase expression can abolish the malignant characteristics of gastric cancer cells.</p> <p>Methods</p> <p>Three heparanase-specific small interfering RNA (siRNAs) were designed, synthesized, and transfected into cultured gastric cancer cell line SGC-7901. Heparanase expression was measured by RT-PCR, real-time quantitative PCR and Western blot. Cell proliferation was detected by MTT colorimetry and colony formation assay. The <it>in vitro </it>invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenesis capabilities of cancer cells were measured by tube formation of endothelial cells.</p> <p>Results</p> <p>Transfection of siRNA against 1496-1514 bp of encoding regions resulted in reduced expression of heparanase, which started at 24 hrs and lasted for 120 hrs post-transfection. The siRNA-mediated silencing of heparanase suppressed the cellular proliferation of SGC-7901 cells. In addition, the <it>in vitro </it>invasion and metastasis of cancer cells were attenuated after knock-down of heparanase. Moreover, transfection of heparanase-specific siRNA attenuated the <it>in vitro </it>angiogenesis of cancer cells in a dose-dependent manner.</p> <p>Conclusions</p> <p>These results demonstrated that gene silencing of heparanase can efficiently abolish the proliferation, invasion, metastasis and angiogenesis of human gastric cancer cells <it>in vitro</it>, suggesting that heparanase-specific siRNA is of potential values as a novel therapeutic agent for human gastric cancer.</p
Small RNAs Targeting Transcription Start Site Induce Heparanase Silencing through Interference with Transcription Initiation in Human Cancer Cells
Heparanase (HPA), an endo-h-D-glucuronidase that cleaves the heparan sulfate chain of heparan sulfate proteoglycans, is overexpressed in majority of human cancers. Recent evidence suggests that small interfering RNA (siRNA) induces transcriptional gene silencing (TGS) in human cells. In this study, transfection of siRNA against −9/+10 bp (siH3), but not −174/−155 bp (siH1) or −134/−115 bp (siH2) region relative to transcription start site (TSS) locating at 101 bp upstream of the translation start site, resulted in TGS of heparanase in human prostate cancer, bladder cancer, and gastric cancer cells in a sequence-specific manner. Methylation-specific PCR and bisulfite sequencing revealed no DNA methylation of CpG islands within heparanase promoter in siH3-transfected cells. The TGS of heparanase did not involve changes of epigenetic markers histone H3 lysine 9 dimethylation (H3K9me2), histone H3 lysine 27 trimethylation (H3K27me3) or active chromatin marker acetylated histone H3 (AcH3). The regulation of alternative splicing was not involved in siH3-mediated TGS. Instead, siH3 interfered with transcription initiation via decreasing the binding of both RNA polymerase II and transcription factor II B (TFIIB), but not the binding of transcription factors Sp1 or early growth response 1, on the heparanase promoter. Moreover, Argonaute 1 and Argonaute 2 facilitated the decreased binding of RNA polymerase II and TFIIB on heparanase promoter, and were necessary in siH3-induced TGS of heparanase. Stable transfection of the short hairpin RNA construct targeting heparanase TSS (−9/+10 bp) into cancer cells, resulted in decreased proliferation, invasion, metastasis and angiogenesis of cancer cells in vitro and in athymic mice models. These results suggest that small RNAs targeting TSS can induce TGS of heparanase via interference with transcription initiation, and significantly suppress the tumor growth, invasion, metastasis and angiogenesis of cancer cells
In Vivo Fluorescence Imaging in the Second Near-Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor Uptake
Cancer imaging requires selective high accumulation of
contrast
agents in the tumor region and correspondingly low uptake in healthy
tissues. Here, by making use of a novel synthetic polymer to solubilize
single-walled carbon nanotubes (SWNTs), we prepared a well-functionalized
SWNT formulation with long blood circulation (half-life of ∼30
h) in vivo to achieve ultrahigh accumulation of ∼30% injected
dose (ID)/g in 4T1 murine breast tumors in Balb/c mice. Functionalization
dependent blood circulation and tumor uptake were investigated through
comparisons with phospholipid-PEG solubilized SWNTs. For the first
time, we performed video-rate imaging of tumors based on the intrinsic
fluorescence of SWNTs in the second near-infrared (NIR-II, 1.1–1.4
μm) window. We carried out dynamic contrast imaging through
principal component analysis (PCA) to immediately pinpoint the tumor
within ∼20 s after injection. Imaging over time revealed increasing
tumor contrast up to 72 h after injection, allowing for its unambiguous
identification. The 3D reconstruction of the SWNTs distribution based
on their stable photoluminescence inside the tumor revealed a high
degree of colocalization of SWNTs and blood vessels, suggesting enhanced
permeability and retention (EPR) effect as the main cause of high
passive tumor uptake of the nanotubes
In Vivo Fluorescence Imaging in the Second Near-Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor Uptake
Cancer imaging requires selective high accumulation of
contrast
agents in the tumor region and correspondingly low uptake in healthy
tissues. Here, by making use of a novel synthetic polymer to solubilize
single-walled carbon nanotubes (SWNTs), we prepared a well-functionalized
SWNT formulation with long blood circulation (half-life of ∼30
h) in vivo to achieve ultrahigh accumulation of ∼30% injected
dose (ID)/g in 4T1 murine breast tumors in Balb/c mice. Functionalization
dependent blood circulation and tumor uptake were investigated through
comparisons with phospholipid-PEG solubilized SWNTs. For the first
time, we performed video-rate imaging of tumors based on the intrinsic
fluorescence of SWNTs in the second near-infrared (NIR-II, 1.1–1.4
μm) window. We carried out dynamic contrast imaging through
principal component analysis (PCA) to immediately pinpoint the tumor
within ∼20 s after injection. Imaging over time revealed increasing
tumor contrast up to 72 h after injection, allowing for its unambiguous
identification. The 3D reconstruction of the SWNTs distribution based
on their stable photoluminescence inside the tumor revealed a high
degree of colocalization of SWNTs and blood vessels, suggesting enhanced
permeability and retention (EPR) effect as the main cause of high
passive tumor uptake of the nanotubes
In Vivo Fluorescence Imaging in the Second Near-Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor Uptake
Cancer imaging requires selective high accumulation of
contrast
agents in the tumor region and correspondingly low uptake in healthy
tissues. Here, by making use of a novel synthetic polymer to solubilize
single-walled carbon nanotubes (SWNTs), we prepared a well-functionalized
SWNT formulation with long blood circulation (half-life of ∼30
h) in vivo to achieve ultrahigh accumulation of ∼30% injected
dose (ID)/g in 4T1 murine breast tumors in Balb/c mice. Functionalization
dependent blood circulation and tumor uptake were investigated through
comparisons with phospholipid-PEG solubilized SWNTs. For the first
time, we performed video-rate imaging of tumors based on the intrinsic
fluorescence of SWNTs in the second near-infrared (NIR-II, 1.1–1.4
μm) window. We carried out dynamic contrast imaging through
principal component analysis (PCA) to immediately pinpoint the tumor
within ∼20 s after injection. Imaging over time revealed increasing
tumor contrast up to 72 h after injection, allowing for its unambiguous
identification. The 3D reconstruction of the SWNTs distribution based
on their stable photoluminescence inside the tumor revealed a high
degree of colocalization of SWNTs and blood vessels, suggesting enhanced
permeability and retention (EPR) effect as the main cause of high
passive tumor uptake of the nanotubes