Preliminary search for a νirus in Dacus oleae Gmel. populations in Northern Greece

Στην περίοδο από Ιούλιο έως Δεκέμβριο 1984 συλλέχΟησαν 4.5 g ακμαίων, 8.5 g υγιών προνυμφών και 0.45 g νεκρών προνυμφών του εντόμου Dacus oleae Gmel. από περιοχές της Βόρειας Ελλάδας, που χαρακτηρίζονταν από Βαριά προσβολή των ελαιοδένδρων από δάκο, στα οποία δεν εφαρμόστηκε χημική καταπολέμηση. Τα δείγματα εξετάσθηκαν για εγκλεισμένους και μη εγκλεισμένους ιούς με τη χρησιμοποίηση φυγοκεντρήσεων, οπτικού και ηλεκτρονικού μικροσκοπίου, ανάλυσης νουκλεϊ νικών οξέων KUI πειράματα μολυσματικότητας. Στα δείγματα των νεκρών προνυμφών, και σε αντίθεση με εκείνα των υγιών προνυμφών και των ακμαίων, εντοπίσθηκαν και απομονώθηκαν ιόμορφα σωμάτια. Τα σωμάτια αυτά είχαν διάμετρο περίπου 35 nm και μερικά ήταν άδεια, όπως φάνηκε από τη διείσδυση της χρωστικής κατά την αρνητική χρώση. Δεν κατέστη δυνατός ο παραπέρα χαρακτηρισμός των «ιοσωματίων» για το λόγο έλλειψης αρκετής ποσότητας δείγματος, ενώ προσπάθειες πολλαπλασιασμούτους σε προνύμφες του λεπιδόπτερου Galleria mellonella και σε καλλιέργειες κυττάρων Drosophila melanogastcr αποδείχθησαν ανεπιτυχείς. Αν και τα μικρά ιόμορφα σωμάτια ήταν το μοναδικό πιθανό παθογόνο αίτιο που αναγνωρίστηκε στις νεκρές προνύμφες, φαίνεται κάπως απίθανο να αποτελούν και το μοναδικό αίτιο του θανάτου για το λόγο του σχετικά μικρού αριθμού τους. Πάντως αν καταστεί δυνατό να πολλαπλασιασθούν οι «ιοί» αυτοί σε εκτροφές του δάκου της ελιάς ή σε καλλιέργειες κυττάρων ιστών του ίδιου εντόμου, ίσως να αποτελέσουν στο μέλλον ένα βιολογικό μέσο καταπολέμησης του.A large number of larvae of Dacus oleae were collected from infested olives in Northern Greece, and a small proportion of these were found to be dead. Adult flies were caught in McPhail traps at the same locations. The larvae and adults were fractionated by a series of steps designed to identify occluded and nonoccluded viruses. Virus-like particles were identified in small amounts only in the dead larvae

A Case of Emmonsiosis in an HIV-Infected Child

Opportunistic fungal infections can cause significant morbidity and mortality in immunocompromised patients. We describe a paediatric case of an unusual disseminated fungal infection. A three-year-old HIV-infected child with severe immunosuppression (CD4+ T-cell count 12 × 106/L) was admitted to hospital with pneumonia, gastroenteritis and herpes gingivostomatitis. Despite antibacterial and antiviral therapy, he experienced high fevers and developed an erythematous maculopapular rash and abdominal tenderness. The child’s condition progressively worsened during the admission. A thermally dimorphic fungus was cultured from bone marrow and identified as an Emmonsia species on DNA sequencing. The patient made a good recovery on amphotericin B deoxycholate and antiretroviral therapy. Itraconazole was continued for a minimum of 12 months, allowing for immune reconstitution to occur. This case is the first documented description of disseminated disease caused by a novel Emmonsia species in an HIV-infected child in South Africa

Prognostic significance of serum inflammatory markers in esophageal cancer

Background The aim of this study was to assess the relative prognostic value of biomarkers to measure the systemic inflammatory response (SIR) and potentially improve prognostic modeling in patients undergoing potentially curative surgery for esophageal adenocarcinoma (EC). Methods Consecutive 330 patients undergoing surgery for EC between 2004 and 2018 within a regional UK cancer network were identified. Serum measurements of haemoglobin, C-reactive protein, albumin, modified Glasgow Prognostic Score (mGPS), and differential neutrophil to lymphocyte ratio (NLR) were obtained before surgery, and correlated with histopathological factors and outcomes. Primary outcome measures were disease-free (DFS) and overall survival (OS). Results Of 330 OC patients, 294 underwent potentially curative esophagectomy. Univariable DFS analysis revealed pT, pN, pTNM stage (all p < 0.001), poor differentiation (p = 0.001), vascular invasion (p < 0.001), R1 status (p < 0.001), perioperative chemotherapy (p = 0.009), CRP (p = 0.010), mGPS (p = 0.011), and NLR (p < 0.001), were all associated with poor survival. Multivariable Cox regression analysis of DFS revealed only NLR [Hazard Ratio (HR) 3.63, 95% Confidence Interval (CI) 2.11–6.24, p < 0.001] retained significance. Multivariable Cox regression analysis of OS revealed similar findings: NLR [HR 2.66, (95% CI 1.58–4.50), p < 0.001]. Conclusion NLR is an important SIR prognostic biomarker associated with DFS and OS in EC

Twins Early Development Study: A Genetically Sensitive Investigation into Behavioral and Cognitive Development from Infancy to Emerging Adulthood

The Twins Early Development Study (TEDS) is a longitudinal twin study that recruited over 16,000 twin-pairs born between 1994 and 1996 in England and Wales through national birth records. More than 10,000 of these families are still engaged in the study. TEDS was and still is a representative sample of the population in England and Wales. Rich cognitive and emotional/behavioral data have been collected from the twins from infancy to emerging adulthood, with data collection at first contact and at ages 2, 3, 4, 7, 8, 9, 10, 12, 14, 16, 18 and 21, enabling longitudinal genetically sensitive analyses. Data have been collected from the twins themselves, from their parents and teachers, and from the UK National Pupil Database. Genotyped DNA data are available for 10,346 individuals (who are unrelated except for 3320 dizygotic co-twins). TEDS data have contributed to over 400 scientific papers involving more than 140 researchers in 50 research institutions. TEDS offers an outstanding resource for investigating cognitive and behavioral development across childhood and early adulthood and actively fosters scientific collaborations

Physiological performance and inflammatory markers as indicators of complications after oesophageal cancer surgery

Background The extent to which physiological factors influence outcome following oesophageal cancer surgery is poorly understood. This study aimed to evaluate the extent to which cardiorespiratory fitness and selected metabolic factors predicted complications after surgery for carcinoma. Methods Two hundred and twenty‐five consecutive patients underwent preoperative cardiopulmonary exercise testing to determine peak oxygen uptake (urn:x-wiley:24749842:media:bjs550328:bjs550328-math-0001o2peak), anaerobic threshold and the ventilatory equivalent for carbon dioxide (urn:x-wiley:24749842:media:bjs550328:bjs550328-math-0002e/urn:x-wiley:24749842:media:bjs550328:bjs550328-math-0003co2). Cephalic venous blood was assayed for serum C‐reactive protein (CRP) and albumin levels, and a full blood count was done. The primary outcome measure was the Morbidity Severity Score (MSS). Results One hundred and ninety‐eight patients had anatomical resection. A high MSS (Clavien–Dindo grade III or above) was found in 48 patients (24·2 per cent) and was related to an increased CRP concentration (area under the receiver operating characteristic (ROC) curve (AUC) 0·62, P = 0·001) and lower urn:x-wiley:24749842:media:bjs550328:bjs550328-math-0004o2peak (AUC 0·36, P = 0·003). Dichotomization of CRP levels (above 10 mg/l) and urn:x-wiley:24749842:media:bjs550328:bjs550328-math-0005o2peak (below 18·6 ml per kg per min) yielded adjusted odds ratios (ORs) for a high MSS of 2·86 (P = 0·025) and 2·92 (P = 0·002) respectively. Compared with a cohort with a low Combined Inflammatory and Physiology Score (CIPS), the OR was 1·70 (95 per cent c.i. 0·85 to 3·39) for intermediate and 27·47 (3·12 to 241·69) for high CIPS (P < 0·001). Conclusion CRP and urn:x-wiley:24749842:media:bjs550328:bjs550328-math-0006o2peak were independently associated with major complications after potentially curative oesophagectomy for cancer. A composite risk score identified a group of patients with a high risk of developing complications

Complex microwave conductivity of Na-DNA powders

We report the complex microwave conductivity, $\sigma=\sigma_1-i\sigma_2$, of Na-DNA powders, which was measured from 80 K to 300 K by using a microwave cavity perturbation technique. We found that the magnitude of $\sigma_1$ near room temperature was much larger than the contribution of the surrounding water molecules, and that the decrease of $\sigma_1$ with decreasing temperature was sufficiently stronger than that of the conduction of counterions. These results clearly suggest that the electrical conduction of Na-DNA is intrinsically semiconductive.Comment: 16 pages, 7 figure

Fluctuation-Facilitated Charge Migration along DNA

We propose a model Hamiltonian for charge transfer along the DNA double helix with temperature driven fluctuations in the base pair positions acting as the rate limiting factor for charge transfer between neighboring base pairs. We compare the predictions of the model with the recent work of J.K. Barton and A.H. Zewail (Proc.Natl.Acad.Sci.USA, {\bf 96}, 6014 (1999)) on the unusual two-stage charge transfer of DNA.Comment: 4 pages, 2 figure