Enhancement of homology-directed repair with chromatin donor templates in cells.

A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, increases the frequency of HDR-edited clones as well as homozygous editing. In addition, transfection of chromatin results in negligible cytotoxicity. These findings suggest that a chromatin donor template should be useful for a wide range of HDR applications such as the precise insertion or replacement of DNA fragments that contain the coding regions of genes

Anomalous phase of MnP at very low field

Manganese phosphide MnP has been investigated for decades because of its rich magnetic phase diagram. It is well known that the MnP exhibits the ferromagnetic phase transition at \Tc=292 K and the helical magnetic phase below \TN=47 K at zero field. Recently, a novel magnetic phase transition was observed at $T^* = 282$ K when the magnetic field is lower than 5 Oe. However, the nature of the new phase has not been illuminated yet. In order to reveal it, we performed the AC and the DC magnetization measurements for a single crystal MnP at very low field. A divergent behavior of the real and the imaginary part of the AC susceptibility and a sharp increase of the DC magnetization was observed at $T^*$, indicating the magnetic phase transition at $T^*$. Furthermore a peculiar temperature hysteresis was observed: namely, the magnetization depends on whether cooling sample to the temperature lower than \TN or not before the measurements. This hysteresis phenomenon suggests the complicated nature of the new phase and a strong relation between the magnetic state of the new phase and the helical structure.Comment: 4 pages, 2 figure

Generation of optimal trajectories for Earth hybrid pole sitters

A pole-sitter orbit is a closed path that is constantly above one of the Earth's poles, by means of continuous low thrust. This work proposes to hybridize solar sail propulsion and solar electric propulsion (SEP) on the same spacecraft, to enable such a pole-sitter orbit. Locally-optimal control laws are found with a semi-analytical inverse method, starting from a trajectory that satisfies the pole-sitter condition in the Sun-Earth circular restricted three-body problem. These solutions are subsequently used as first guess to find optimal orbits, using a direct method based on pseudospectral transcription. The orbital dynamics of both the pure SEP case and the hybrid case are investigated and compared. It is found that the hybrid spacecraft allows savings on propellant mass fraction. Finally, it is shown that for sufficiently long missions, a hybrid pole-sitter, based on mid-term technology, enables a consistent reduction in the launch mass for a given payload, with respect to a pure SEP spacecraft

A dramatic isotope effect in the reaction of ClSiH with trimethylsilane-1-d: experimental evidence for intermediate complexes in silylene Si-H(D) insertion reactions

A kinetic isotope effect (kD/kH) of 7.4 has been found for the reaction of chlorosilylene with trimethysilane (Me3SiD vs Me3SiH). Such a value can be accounted for by theoretical modelling, but only if an internal rearrangement of the initially form complex is included in the mechanism. This provides the first concrete evidence for such complexes