332 research outputs found
Amylase release from streptolysin O-permeabilized pancreatic acinar cells. Effects of Ca2+, guanosine 5'-[gamma-thio]triphosphate, cyclic AMP, tetanus toxin and botulinum A toxin
The molecular requirements for amylase release and the intracellular effects of botulinum A toxin and tetanus toxin on amylase release were investigated using rat pancreatic acinar cells permeabilized with streptolysin O. Micromolar concentrations of free Ca2+ evoked amylase release from these cells. Maximal release was observed in the presence of 30 microM free Ca2+. Ca(2+)-stimulated, but not basal, amylase release was enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[S]) (3-4 fold) or cyclic AMP (1.5-2 fold). Neither the two-chain forms of botulinum A toxin and tetanus toxin, under reducing conditions, nor the light chains of tetanus toxin, inhibited amylase release triggered by Ca2+, or combinations of Ca2+ + GTP[S] or Ca2+ + cAMP. The lack of inhibition was not due to inactivation of botulinum A toxin or tetanus toxin by pancreatic acinar cell proteolytic enzymes, as toxins previously incubated with permeabilized pancreatic acinar cells inhibited Ca(2+)-stimulated [3H]noradrenaline release from streptolysin O-permeabilized adrenal chromaffin cells. These data imply that clostridial neurotoxins inhibit a Ca(2+)-dependent mechanism which promotes exocytosis in neural and endocrine cells, but not in exocrine cells
Applying weighted network measures to microarray distance matrices
In recent work we presented a new approach to the analysis of weighted
networks, by providing a straightforward generalization of any network measure
defined on unweighted networks. This approach is based on the translation of a
weighted network into an ensemble of edges, and is particularly suited to the
analysis of fully connected weighted networks. Here we apply our method to
several such networks including distance matrices, and show that the clustering
coefficient, constructed by using the ensemble approach, provides meaningful
insights into the systems studied. In the particular case of two data sets from
microarray experiments the clustering coefficient identifies a number of
biologically significant genes, outperforming existing identification
approaches.Comment: Accepted for publication in J. Phys.
An ensemble approach to the analysis of weighted networks
We present a new approach to the calculation of measures in weighted
networks, based on the translation of a weighted network into an ensemble of
edges. This leads to a straightforward generalization of any measure defined on
unweighted networks, such as the average degree of the nearest neighbours, the
clustering coefficient, the `betweenness', the distance between two nodes and
the diameter of a network. All these measures are well established for
unweighted networks but have hitherto proven difficult to define for weighted
networks. Further to introducing this approach we demonstrate its advantages by
applying the clustering coefficient constructed in this way to two real-world
weighted networks.Comment: 4 pages 3 figure
General implementation of all possible positive-operator-value measurements of single photon polarization states
Positive Operator Value Measures (POVMs) are the most general class of
quantum measurements. We propose a setup in which all possible POVMs of a
single photon polarization state (corresponding to all possible sets of
two-dimensional Kraus operators) can be implemented easily using linear optics
elements. This method makes it possible to experimentally realize any
projective orthogonal, projective non-orthogonal or non-projective sets of any
number of POVM operators. Furthermore our implementation only requires vacuum
ancillas, and is deterministic rather than probabilistic. Thus it realizes
every POVM with the correct set of output states. We give the settings required
to implement two different well-known non-orthogonal projective POVMs.Comment: 5 pages, newer version with minor addition
Nondeterministic self-assembly with asymmetric interactions.
We investigate general properties of nondeterministic self-assembly with asymmetric interactions, using a computational model and DNA tile assembly experiments. By contrasting symmetric and asymmetric interactions we show that the latter can lead to self-limiting cluster growth. Furthermore, by adjusting the relative abundance of self-assembly particles in a two-particle mixture, we are able to tune the final sizes of these clusters. We show that this is a fundamental property of asymmetric interactions, which has potential applications in bioengineering, and provides insights into the study of diseases caused by protein aggregation.Winton Programme for the Physics of Sustainability, Gates Cambridge, Oppenheimer PhD studentship, NanoDTC Cambridge (Grant ID: EP/L015978/1), Engineering and Physical Sciences Research Council (Grant ID: EP/L504920/1), Royal SocietyThis is the author accepted manuscript. The final version is available from the American Physical Society via http://dx.doi.org/10.1103/PhysRevE.94.02240
Introduction of Macromolecules into Bovine Adrenal Medullary Chromaffin Cells and Rat Pheochromocytoma Cells (PC12) by Permeabilization with Streptolysin O: Inhibitory Effect of Tetanus Toxin on Catecholamine Secretion
Conditions are described for controlled plasma membrane permeabilization of rat pheochromocytoma cells (PC12) and cultured bovine adrenal chromaffin cells by Streptolysin O (SLO). The transmembrane pores created by SLO invoke rapid efflux of intracellular 86Rb+ and ATP, and also permit passive diffusion of proteins, including immunoglobulins, into the cells. SLO-permeabilized PC12 cells release [3H]dopamine in response to micromolar concentrations of free Ca2+. Permeabilized adrenal chromaffin cells present a similar exocytotic response to Ca2+ in the presence of Mg2+/ ATP. Permeabilized PC12 cells accumulate antibodies against synaptophysin and calmodulin, but neither antibody reduces the Ca2+-dependent secretory response. Reduced tetanus toxin, although ineffective when applied to intact chromaffin cells, inhibits Ca2+-induced exocytosis by both types of permeabilized cells studied. Omission of dithiothreitol, toxin inactivation by boiling, or preincubation with neutralizing antibodies abolishes the inhibitory effect. The data indicate that plasma membrane permeabilization by Streptolysin O is a useful tool to probe and define cellular components that are involved in the final steps of exocytosis
Gradient structures for flows of concentrated suspensions
In this work we investigate a two-phase model for concentrated suspensions. We construct a PDE formulation using a gradient flow structure featuring dissipative coupling between fluid and solid phase as well as different driving forces. Our construction is based on the concept of flow maps that also allows it to account for flows in moving domains with free boundaries. The major difference compared to similar existing approaches is the incorporation of a non-smooth two-homogeneous term to the dissipation potential, which creates a normal pressure even for pure shear flows
Low-temperature behaviour of social and economic networks
Real-world social and economic networks typically display a number of particular topological properties, such as a giant connected component, a broad degree distribution, the small-world property and the presence of communities of densely interconnected nodes. Several models, including ensembles of networks, also known in social science as Exponential Random Graphs, have been proposed with the aim of reproducing each of these properties in isolation. Here, we define a generalized ensemble of graphs by introducing the concept of graph temperature, controlling the degree of topological optimization of a network. We consider the temperature-dependent version of both existing and novel models and show that all the aforementioned topological properties can be simultaneously understood as the natural outcomes of an optimized, low-temperature topology. We also show that seemingly different graph models, as well as techniques used to extract information from real networks are all found to be particular low-temperature cases of the same generalized formalism. One such technique allows us to extend our approach to real weighted networks. Our results suggest that a low graph temperature might be a ubiquitous property of real socio-economic networks, placing conditions on the diffusion of information across these systems. © 2013 by the authors
Further Characterization of Dopamine Release by Permeabilized PC 12 Cells
Rat pheochromocytoma cells (PC 12) permeabilized with staphylococcal α-toxin release [3H]dopamine after addition of micromolar Ca2+. This does not require additional Mg2+-ATP (in contrast to bovine adrenal medullary chromaffin cells). We also observed Ca2+-dependent [3H]-dopamine release from digitonin-permeabilized PC 12 cells. Permeabilization with α-toxin or digitonin and stimulation of the cells were done consecutively to wash out endogenous Mg2+-ATP. During permeabilization, ATP was removed effectively from the cytoplasm by both agents but the cells released [3H]dopamine in response to micromolar Ca2+ alone. Replacement by chloride of glutamate, which could sustain mitochondrial ATP production in permeabilized cells, does not significantly alter catecholamine release induced by Ca2+. However, Mg2+ without ATP augments the Ca2+-induced release. The release was unaltered by thiol-, hydroxyl-, or calmodulin-interfering substances. Thus Mg2+-ATP, calmodulin, or proteins containing -SH or -OH groups are not necessary for exocytosis in permeabilized PC 12 cells
Synchronization of Sound Sources
Sound generation and -interaction is highly complex, nonlinear and
self-organized. Already 150 years ago Lord Rayleigh raised the following
problem: Two nearby organ pipes of different fundamental frequencies sound
together almost inaudibly with identical pitch. This effect is now understood
qualitatively by modern synchronization theory (M. Abel et al., J. Acoust. Soc.
Am., 119(4), 2006). For a detailed, quantitative investigation, we substituted
one pipe by an electric speaker. We observe that even minute driving signals
force the pipe to synchronization, thus yielding three decades of
synchronization -- the largest range ever measured to our knowledge.
Furthermore, a mutual silencing of the pipe is found, which can be explained by
self-organized oscillations, of use for novel methods of noise abatement.
Finally, we develop a specific nonlinear reconstruction method which yields a
perfect quantitative match of experiment and theory.Comment: 5 pages, 4 figure
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