DNA methylation clock DNAmFitAge shows regular exercise is associated with slower aging and systemic adaptation

DNAmPhenoAge, DNAmGrimAge, and the newly developed DNAmFitAge are DNA methylation (DNAm)-based biomarkers that reflect the individual aging process. Here, we examine the relationship between physical fitness and DNAm-based biomarkers in adults aged 33–88 with a wide range of physical fitness (including athletes with long-term training history). Higher levels of VO 2 max ( ρ = 0.2, p = 6.4E − 4, r = 0.19, p = 1.2E − 3), Jumpmax ( p = 0.11, p = 5.5E − 2, r = 0.13, p = 2.8E − 2), Gripmax ( ρ = 0.17, p = 3.5E − 3, r = 0.16, p = 5.6E − 3), and HDL levels ( ρ = 0.18, p = 1.95E − 3, r = 0.19, p = 1.1E − 3) are associated with better verbal short-term memory. In addition, verbal short-term memory is associated with decelerated aging assessed with the new DNAm biomarker FitAgeAcceleration ( ρ : − 0.18, p = 0.0017). DNAmFitAge can distinguish high-fitness individuals from low/medium-fitness individuals better than existing DNAm biomarkers and estimates a younger biological age in the high-fit males and females (1.5 and 2.0 years younger, respectively). Our research shows that regular physical exercise contributes to observable physiological and methylation differences which are beneficial to the aging process. DNAmFitAge has now emerged as a new biological marker of quality of life

Tisztítószergyártás során keletkezett elfolyóvizek vizsgálata és kezelési lehetőségei mezokozmosz kísérlet alapján

A RO koncentrátum és tisztított szennyvíz különböző arányú keveréke közvetlenül nem vezethető be a Kettős-Körös vizébe. Azt előzetes célszerű egy erre a célra kialakított vizes élőhelyen, a csapadék vízzel hígítva biológiai öntisztulásnak alávetni. A gyártelep területéről összefolyó csapadék víz mennyisége elégséges az RO koncentrátum és tisztított szennyvíz oly mértékű hígításához, hogy az megfelelő levegőztetés mellett biológiai tisztuláson menjen keresztül.MscHidrobiológusg

Színes bogyós gyümölcsök antioxidáns paramétereinek, in vitro teszt és sejtkultúrás jellemzőinek vizsgálata

Különböző színes bogyós gyümölcsök antioxidáns kémiai analitikai vizsgálata, majd a kiválasztott minták tesztelése NCM460 normál vastagbélnyálkahártya és Caco-2 vastagbél adenokarcinóma sejtvonalakon.MscBiológusg

Tisztítószergyártás során keletkezett elfolyóvizek vizsgálata és kezelési lehetőségei mezokozmosz kísérlet alapján

A RO koncentrátum és tisztított szennyvíz különböző arányú keveréke közvetlenül nem vezethető be a Kettős-Körös vizébe. Azt előzetes célszerű egy erre a célra kialakított vizes élőhelyen, a csapadék vízzel hígítva biológiai öntisztulásnak alávetni. A gyártelep területéről összefolyó csapadék víz mennyisége elégséges az RO koncentrátum és tisztított szennyvíz oly mértékű hígításához, hogy az megfelelő levegőztetés mellett biológiai tisztuláson menjen keresztül.MscHidrobiológusg

The Flavonoid Rich Black Currant (<i>Ribes nigrum</i>) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum <i>TNF-α</i> Level: Pilot Study

Many plant-derived flavonoids are known for their anti-neuroinflammatory and anti-neurodegenerative effects. The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits. The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds. It provides details about the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties. The reported BC-GTE was found to contain approximately 133 phytonutrients, making it unique in its composition. Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol. Drosophila melanogaster-based tests revealed no cytotoxic but nutritive effects. We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident. Moreover, no elevated levels of serum-specific TNF-α were observed under the LPS-induced neuroinflammatory condition. The analyzed BC-GTE’s specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties. This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach

Effects of Long-Term Moderate Intensity Exercise on Cognitive Behaviors and Cholinergic Forebrain in the Aging Rat

Physical exercise is now generally considered as a strategy to maintain cognitive abilities and to prevent age-related cognitive decline. In the present study, Wistar rats were subjected to moderate intensity treadmill exercise for 6 months prior to sacrifice at 12-, 24- and 32-month of age. This chronic physical intervention was tested on motility in the Open field (OF). Cognitive functions were measured in the Morris water maze (MWM) for spatial learning and in the Novel object recognition (NOR) tests. Since learning and memory are closely associated with cholinergic forebrain function ChAT fiber density after exercise training was assessed in hippocampus, and motor- and somatosensory cortical areas. Furthermore, quantification of ChAT-positive fiber aberrations as a neuropathological marker was also carried out in these brain areas. Our results show that in OF chronic exercise maintained horizontal locomotor activity in all age groups. Rearing activity, MWM and notably NOR performance were improved only in the 32-months old animals. Regarding cholinergic neuronal innervation, apart from a general age-related decline, exercise increased ChAT fiber density in the hippocampus CA1 area and in the motor cortex notably in the 32-months group. Massive ChAT fiber aberrations in all investigated areas which developed in senescence were clearly attenuated by exercise. The results suggest that moderate intensity chronic exercise in the rat is especially beneficial in advanced age. In conclusion, chronic exercise attenuates the age-related decline in cognitive and motor behaviors as well as age-related cholinergic fiber reduction, reduces malformations of cholinergic forebrain innervation. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved