PENINGKATAN PARAMETER HEMORHEOLOGI DAN C-REACTIVE PROTEIN BERKORELASI DENGAN BERBAGAI DERAJAT STENOSIS JANTUNG KORONER

Background : Endotel dysfunction plays important role in the atherosclerosis formation before the inflammation process occurs. This endotel dysfunction is strongly related to the increment of blood viscosity (BV) thorough shear stress changes. C-reactive protein (CRP) is a biomarker inflammation and plays as a key in endotel dysfunction, formation and atherosclerosis plaque's progression. Objective : To know about the relation among BV and CRP with various degree of cardiac coronary stenosis. Method : There were twenty four patients with coronary heart disease who were taken angiography. BV levels were checked using Oswald viscometer and CRP by turbidimetric imunoassay. Angiography examination had been used to determine degree of coronary stenosis. The data had been analysed by using the Spearman correlation index. Result : The subject of this research consist of 17 men and 7 women with age in average of 55,96±7,29 years. It is found by angiography examination that there were 2 subjects (8,3%) non-significant stenosis, 22 others (91,7%) significant stenosis, and no one of them had normal stenosis. The correlation between BV and degree of coronary stenosis was moderate (r = 0,549; p = 0,005). The correlation between CRP and degree of coronary stenosis was moderate as well (r = 0,481; p = 0,017). Conclusion : There is an moderate positive correlation between BV and the degree of cardiac coronary stenosis. The similar correlation occurs between CRP and those degrees as well. Keywords : coronary heart disease, endotel dysfunction, C-reactive protein (CRP), blood viscosity, atherosclerosis, shear stress

A trombocita aggregáció és teljes vér viszkozitás mérésének jelentősége az alapkutatásban és a klinika gyakorlatban

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Haemorheologiai és haemostaselogiai tényezők szerepe ischaemiás cerebrovascularis kórképekben

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Experimental investigation of the ophthalmological effects of the pituitary cyclase activating polypeptide (PACAP)

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BLOOD VISCOSITY AS A DETERMINING FACTOR OF ISCHEMIC STROKE OUTCOMES EVALUATED WITH NIHSS AND MRS ON DAY 7 AND 30 POST-THROMBOLYSIS

Objective: This study aimed to analyze blood viscosity as a determining factor of ischemic stroke outcomes evaluated with National Institutes of Health Stroke Scale (NIHSS) and modified Rankin Scale (mRS) on day 7 and 30 post-thrombolysis. Methods: This study was a 4-months cohort study taking place in Cipto Mangunkusumo General Hospital from January to April 2017. Subjects were collected at the Emergency Department or Neurology Outpatient Department. Eligible patients gave informed consent. Patients underwent numerous examinations, including blood viscosity test using digital microcapillary (DM) instrument. Outcomes of patients were identified on day 7 and day 30 post-thrombolysis using NIHSS and mRS, respectively. Results: Most acute ischemic stroke patients (88.6%) had blood hyperviscosity. 9.1% patients had poorer neurologic deficit on day 7 evaluated with NIHSS and 18.2% patients had poor outcome on day 30 evaluated with mRS. All patients with normal blood viscosity did not have a poorer neurologic deficit on day-7-evaluation. Conclusion: Blood viscosity determines the outcomes of acute ischemic stroke patients on day 7 and day 30 post-thrombolysis

Genome-wide association and linkage analyses of hemostatic factors and hematological phenotypes in the Framingham Heart Study

<p>Abstract</p> <p>Background</p> <p>Increased circulating levels of hemostatic factors as well as anemia have been associated with increased risk of cardiovascular disease (CVD). Known associations between hemostatic factors and sequence variants at genes encoding these factors explain only a small proportion of total phenotypic variation. We sought to confirm known putative loci and identify novel loci that may influence either trait in genome-wide association and linkage analyses using the Affymetrix GeneChip 100K single nucleotide polymorphism (SNP) set.</p> <p>Methods</p> <p>Plasma levels of circulating hemostatic factors (fibrinogen, factor VII, plasminogen activator inhibitor-1, von Willebrand factor, tissue plasminogen activator, D-dimer) and hematological phenotypes (platelet aggregation, viscosity, hemoglobin, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration) were obtained in approximately 1000 Framingham Heart Study (FHS) participants from 310 families. Population-based association analyses using the generalized estimating equations (GEE), family-based association test (FBAT), and multipoint variance components linkage analyses were performed on the multivariable adjusted residuals of hemostatic and hematological phenotypes.</p> <p>Results</p> <p>In association analysis, the lowest GEE p-value for hemostatic factors was p = 4.5*10^-16for factor VII at SNP rs561241, a variant located near the <it>F7 </it>gene and in complete linkage disequilibrium (LD) (r²= 1) with the Arg353Gln <it>F7 </it>SNP previously shown to account for 9% of total phenotypic variance. The lowest GEE p-value for hematological phenotypes was 7*10^-8at SNP rs2412522 on chromosome 4 for mean corpuscular hemoglobin concentration. We presented top 25 most significant GEE results with p-values in the range of 10^-6to 10^-5for hemostatic or hematological phenotypes. In relating 100K SNPs to known candidate genes, we identified two SNPs (rs1582055, rs4897475) in erythrocyte membrane protein band 4.1-like 2 (<it>EPB41L2</it>) associated with hematological phenotypes (GEE p < 10^-3). In linkage analyses, the highest linkage LOD score for hemostatic factors was 3.3 for factor VII on chromosome 10 around 15 Mb, and for hematological phenotypes, LOD 3.4 for hemoglobin on chromosome 4 around 55 Mb. All GEE and FBAT association and variance components linkage results can be found at <url>http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?id=phs000007</url></p> <p>Conclusion</p> <p>Using genome-wide association methodology, we have successfully identified a SNP in complete LD with a sequence variant previously shown to be strongly associated with factor VII, providing proof of principle for this approach. Further study of additional strongly associated SNPs and linked regions may identify novel variants that influence the inter-individual variability in hemostatic factors and hematological phenotypes.</p

Болезнь мелких сосудов: патогенетические подтипы, возможные лечебные стратегии.

В обзоре рассмотрены клинические и нейровизуализационные критерии состояний, объединяемых в группу болезней мелких сосудов головного мозга, и отличия терапевтических возможностей их лечения для профилактики прогрессирования неврологических симптомов, когнитивного снижения от иных причин острой и хронической ишемии головного мозга. Авторы рассматривают потенциал новой формы выпуска - диспергируемые таблетки винпоцетина (Кавинтон Комфорте) как препарата, способного оказать воздействие на разные патогенетические факторы, содействующие прогрессированию болезни мелких сосудов