Aged (Black) versus Raw Garlic against Ischemia/Reperfusion-Induced Cardiac Complications

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bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues

High molecular weight glutenin subunits (HMW GS) represent an important fraction of endosperm-specific seed-storage proteins that provide elasticity to bread dough. Previously, the second cis-regulatory module (CRM2) was found to be one of the most conserved part of HMW GS promoters, which indicated its pre-eminent role in their gene regulation. Here, we observed that deletion of CRM2 from the promoters of the Bx7 and By8 HMW GS genes increased the leakage of their transient expression in wheat leaf tissue. The effect of a VP1, an Myb and an antisense bZIP transcription factor (TF)-binding site, potentially involved in endosperm-specific regulation within CRM2, was then studied. The activity of a Bx7 gene promoter containing a mutant CRM2 with altered VP1 and Myb TF-binding sites, but an intact bZIP TF-binding site, was similarly low to that of the wild type in leaves. Transactivation analysis and EMSA indicated the binding of TFs TabZIP34 and TabZIP115 to the Skn-1 motif GTCAT in CRM2 and the repression of Bx7 and By8 HMW GS gene promoter activity in leaves. TabZIP34 and TabZIP115 may be involved in the downregulation of HMW GS gene expression in vegetative tissues and early-stage endosperm as well its modulation during seed maturation

Dataset on structure, stability and myocardial effects of a new hybrid aspirin containing nitrogen monoxide-releasing molsidomine moiety

Herein 1H and 13C NMR spectra of ERJ-500, a new hybrid aspirin derivative, covalently conjugated to nitrogen monoxide donor linsidomine are presented as well as NMR spectra of its synthetic intermediate compounds. HPLC-MS measurements data are also included, demonstrating the stability of the linsidomine-aspirin hybrid in oxidation reactions. This data article also concerns miscellaneous myocardial parameters of isolated rat hearts as a complementation of the tables shown in the paper entitled “A new, vasoactive hybrid aspirin containing nitrogen monoxidereleasing molsidomine moiety” Szoke et al., 2019. Column tables represent data of aorta flow, aortic pressure, derivated aortic pressure and cardiac output

A NEW, VASOACTIVE HYBRID ASPIRIN CONTAINING NITROGEN MONOXIDE-RELEASING MOLSIDOMINE MOIETY

Ischemic heart conditions are among the main causes of sudden cardiac death worldwide. One of the strategies for avoiding myocardial infarction is the low-dose, prophylactic use of acetylsalicylic acid (ASA), an inhibitor of platelet aggregation. To avoid the gastrointestinal damage, ASA prodrugs bearing nitric oxide (NO)-donating moiety covalently conjugated to ASA have been synthesized and evaluated extensively worldwide. Herein the synthesis of a new hybrid ASA ester covalently attached to the NO donor linsidomine, an active metabolite of molsidomine (MOL) is reported. Cell viability assay and hemolysis tests were performed in H9c2 cells and rat erythrocytes, respectively. Our new compound, the ERJ-500 not affected negatively the viability of living cells in the concentration range of 100 nM to 100 µM. Using the ex vivo Langendorff method on hearts originated from female rats, compound ERJ-500 displayed a dose-dependent, outwashable vasodilative effect in coronary arteries. Vasodilation was observed on isolated working heart model as well, with elevated stroke volume in hearts treated with ERJ-500. Furthermore, a decreased infarct size was also noticed in ERJ-500 treated hearts after ischemia/reperfusion. Based on these observations it can be expected that our new hybrid ASA may contribute to new pharmacological tool in the therapy of ischemic heart conditions and associated syndromes

Az angiotenzin-II hatása az autofágiás folyamatokra H9c2 sejtvonalon

Kísérleteinket H9c2 kardiomioblaszt sejtvonalon végeztük. Angiotenzin-II kezeléssel kívántuk a hipertrófiát kiváltani, ugyanis ez a vazoaktív molekula központi szerepet játszik a hipertrófia patogenezisében. Munkánk során vizsgálni kívántuk az angiotenzin-II kezelés hatását a H9c2 sejtek méretbeli változására, továbbá tanulmányoztuk a kezelés hatását az autofágia és az apoptózis folyamatára.egységes, osztatlangyógyszerésztudományimagyarnappalig

A közlekedési bűncselekmények főbb jellemzői

Szakdolgozatom témájaként a közlekedési bűncselekményeket választottam, mivel véleményem szerint a mai felgyorsult világban egyre nagyobb szerephez jut a közlekedés, ami a mindennapi életünk egyik legnagyobb veszélyforrását jelenti. Nem telik el úgy nap, hogy ne hallanánk a televízióban, rádióban; ne olvasnánk az interneten, folyóiratokban közlekedési bűncselekményekről, balesetekről.Igaz, hogy a közlekedési járművek technikailag egyre fejlettebbek, egyre biztonságosabbak, de ennek ellenére manapság is történhet baleset műszaki hiba miatt.egységes, osztatlanjogás