42 research outputs found
Polyphenols Sensitization Potentiates Susceptibility of MCF-7 and MDA MB-231 Cells to Centchroman
Polyphenols as âsensitizersâ together with cytotoxic drugs as âinducersâ cooperate to trigger apoptosis in various cancer cells. Hence, their combination having similar mode of mechanism may be a novel approach to enhance the efficacy of inducers. Additionally, this will also enable to achieve the physiological concentrations facilitating significant increase in the activity at concentrations which the compound can individually provide. Here we propose that polyphenols (Resveratrol (RES) and Curcumin (CUR)) pre-treatment may sensitize MCF-7/MDA MB-231 (Human Breast Cancer Cells, HBCCs) to Centchroman (CC, antineoplastic agent). 6 h pre-treated cells with 10 ”M RES/CUR and 100 ”M RES/30 ”M CUR doses, followed by 10 ”M CC for 18 h were investigated for Ser-167 ER-phosphorylation, cell cycle arrest, redox homeostasis, stress activated protein kinase (SAPKs: JNK and p38 MAPK) pathways and downstream apoptosis effectors. Low dose RES/CUR enhances the CC action through ROS mediated JNK/p38 as well as mitochondrial pathway in MCF-7 cells. However, RES/CUR sensitization enhanced apoptosis in p53 mutant MDA MB-231 cells without/with involvement of ROS mediated JNK/p38 adjunct to Caspase-9. Contrarily, through high dose sensitization in CC treated cells, the parameters remained unaltered as in polyphenols alone. We conclude that differential sensitization of HBCCs with low dose polyphenol augments apoptotic efficacy of CC. This may offer a novel approach to achieve enhanced action of CC with concomitant reduction of side effects enabling improved management of hormone-dependent breast cancer
Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions
A simple, rapid, and sensitive fluorimetric method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 nm and 508 nm, respectively. The calibration graph was linear from 0.1 to 0.6 ”g/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 ”g/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.Um mĂ©todo fluorimĂ©trico simples, rĂĄpido e sensĂvel foi desenvolvido e validado para quantificação da curcumina em suspensĂ”es de nanocĂĄpsulas lipĂdicas e polimĂ©ricas, usando acetonitrila como solvente. Os comprimentos de onda de excitação e emissĂŁo foram 397 nm e 508 nm, respectivamente. Nas condiçÔes testadas, a curva de calibração demonstrou-se linear na faixa de 0,1 a 0,6 ”g/mL, exibindo coeficiente de correlação de 0,9982. Os limites de detecção e quantificação foram 0,03 e 0,10 ”g/mL, respectivamente. Os resultados da validação confirmaram que o mĂ©todo desenvolvido Ă© especĂfico, linear, exato e preciso para o uso proposto. O presente mĂ©todo foi aplicado com sucesso para a avaliação do teor de curcumina nas suspensĂ”es de nanocĂĄpsulas lipĂdicas e polimĂ©ricas durante o estĂĄgio inicial do desenvolvimento da formulação