A sulfated glycoprotein synthesized by Sertoli cells and by epididymal cells is a component of the sperm membrane

We report here the purification, partial characterization and immunofluorescent localization of a dimeric acidic glycoprotein (DAG-protein) secreted by cultures of Sertoli cells of rat testes. Partially purified protein was obtained after chromatography over Sepharose 4B under conditions which favored a soluble, nonaggregated form of the protein. Rechromatography over the same column under reducing conditions yielded very pure monomers of 41,000 daltons and 29,000 daltons. Antibodies were prepared against the mixed monomers and used to immunoprecipitate proteins in spent medium from cultures incubated with [35S] methionine, 35SO4 = or tunicamycin. DAG-protein and another protein (Band 4, 70,000 daltons) were coprecipitated by the antiserum and all contained 35SO4 = in their structures. It was shown by Western blotting that the antiserum cross-reacted very weakly with Band 4 protein. The DAG-protein polypeptides secreted in the presence of tunicamycin were assumed to lack N-glycosylation and exhibited apparent molecular weights of 27,000 and 21,000 daltons. Immunoprecipitations of media from organ cultures of testis and epididymis yielded DAG-protein of slightly lower molecular weight than the protein secreted in Sertoli cell cultures. Indirect immunofluorescence of DAG-protein in paraffin sections of testis and epididymis revealed that the protein was concentrated in the cytoplasm of Sertoli cells, on the stereocilia of epididymal principal cells, in the cytoplasm of epididymal halo cells, and was associated with late spermatids and mature sperm. Sperm were specifically labeled on the acrosome, at the neck, and on the endpiece of the tail. No enzymatic or structural function has been ascribed to DAG-protein as yet, but the protein must play a pivotal role in spermatogenesis because it is secreted by both the testis and epididymis and becomes an integral component of sperm

Discovery of Reflection Nebulosity Around Five Vega-like Stars

Coronagraphic optical observations of six Vega-like stars reveal reflection nebulosities, five of which were previously unknown. The nebulosities illuminated by HD 4881, HD 23362, HD 23680, HD 26676, and HD 49662 resemble that of the Pleiades, indicating an interstellar origin for dust grains. The reflection nebulosity around HD 123160 has a double-arm morphology, but no disk-like feature is seen as close as 2.5 arcsec from the star in K-band adaptive optics data. We demonstrate that uniform density dust clouds surrounding HD 23362, HD 23680 and HD 123160 can account for the observed 12-100 micron spectral energy distributions. For HD 4881, HD 26676, and HD 49662 an additional emission source, such as from a circumstellar disk or non-equilibrium grain heating, is required to fit the 12-25 micron data. These results indicate that in some cases, particularly for Vega-like stars located beyond the Local Bubble (>100 pc), the dust responsible for excess thermal emission may originate from the interstellar medium rather than from a planetary debris system.Comment: The Astrophysical Journal, in press for March, 2002 (32 pages, 13 figures

Population-scale organization of cerebellar granule neuron signaling during a visuomotor behavior.

Granule cells at the input layer of the cerebellum comprise over half the neurons in the human brain and are thought to be critical for learning. However, little is known about granule neuron signaling at the population scale during behavior. We used calcium imaging in awake zebrafish during optokinetic behavior to record transgenically identified granule neurons throughout a cerebellar population. A significant fraction of the population was responsive at any given time. In contrast to core precerebellar populations, granule neuron responses were relatively heterogeneous, with variation in the degree of rectification and the balance of positive versus negative changes in activity. Functional correlations were strongest for nearby cells, with weak spatial gradients in the degree of rectification and the average sign of response. These data open a new window upon cerebellar function and suggest granule layer signals represent elementary building blocks under-represented in core sensorimotor pathways, thereby enabling the construction of novel patterns of activity for learning

A Resolved Molecular Gas Disk around the Nearby A Star 49 Ceti

The A star 49 Ceti, at a distance of 61 pc, is unusual in retaining a substantial quantity of molecular gas while exhibiting dust properties similar to those of a debris disk. We present resolved observations of the disk around 49 Ceti from the Submillimeter Array in the J=2-1 rotational transition of CO with a resolution of 1.0x1.2 arcsec. The observed emission reveals an extended rotating structure viewed approximately edge-on and clear of detectable CO emission out to a distance of ~90 AU from the star. No 1.3 millimeter continuum emission is detected at a 3-sigma sensitivity of 2.1 mJy/beam. Models of disk structure and chemistry indicate that the inner disk is devoid of molecular gas, while the outer gas disk between 40 and 200 AU from the star is dominated by photochemistry from stellar and interstellar radiation. We determine parameters for a model that reproduces the basic features of the spatially resolved CO J=2-1 emission, the spectral energy distribution, and the unresolved CO J=3-2 spectrum. We investigate variations in disk chemistry and observable properties for a range of structural parameters. 49 Ceti appears to be a rare example of a system in a late stage of transition between a gas-rich protoplanetary disk and a tenuous, virtually gas-free debris disk.Comment: 11 pages, 6 figures, accepted for publication in Ap

The politics of linking educational research, policy, and practice: The case of improving educational quality in Ghana, Guatemala and Mali

This paper examines the political dimension of the educational research undertaken by Ghanian,\ud Guatemalan, and Malian teams as part of the 1991 to 1996, USAID-funded “Improving Educational\ud Quality” (IEQ) project. The following questions are addressed: (a) why were (or were not) aspects\ud of the educational reforms studied by the researchers; (b) why were (or were not) research findings\ud used in decision-makingabout the educationalpolicies and practices associatedwith the reforms; and\ud (c) why were particular institutional arrangements and funding levels constituted for the research and\ud dialogue activity. In offering answers to these questions, attentionis paid to local, national, and global\ud power relations and resource distributions

The Papoose Flat Pluton of eastern California: a reassessment of its emplacement history in the light of new microstructural and crystallographic fabric observations

One of the most outstanding apparent examples in N America of a forcibly emplaced pluton is the Papoose Flat Pluton of eastern California. Sideways expansion of this granitic pluton, during emplacement into a series of Cambrian shelf strata, has been regarded by early workers as resulting in the observed intense crystal plastic deformation of the pluton's mylonitic border facies and surrounding country rocks. This deformation is evidenced by up to 90% thinning of individual stratigraphic layers within the pluton's metamorphic aureole, although such intense penetrative deformation of the country rocks is not observed outside the aureole. Previously published quartz c-axis fabrics associated with this deformation (and presented on projection planes oriented perpendicular to lineation) were interpreted as being symmetrical with respect to foliation and lineation, implying almost coaxial deformation histories. Such fabrics could be interpreted as indicating that the pluton evolved by "ballooning” as a result of new magma being intruded into its core during emplacement. However, a major problem with applying the strict ballooning model to the Papoose Flat Pluton is that while oblate strains would be expected to develop in association with a ballooning mechanism, the mylonitic rocks of this elongate WNW-ESE-trending pluton and its aureole are characterised by both a strongly developed foliation, which is concordant with the pluton's margin, and an intense, NW-SE trending, shallow plunging stretching lineation. Previously published fabrics from the Papoose Flat Pluton and its metamorphic aureole have been rotated on to a projection plane oriented parallel to lineation and perpendicular to foliation. Examination of the fabrics in this projection plane has revealed that they are in fact dominantly asymmetric, and that a constant sense of asymmetry is detected across the pluton, suggesting a consistent (top-to-the-SE) shear-sense. This new interpretation is strongly supported by microstructural and petrofabric analysis of additional L-S tectonites collected, during recent fieldwork, from both the aureole and quartz veins within the pluton's gneissic border facies. Thus mylonite formation around the Papoose Flat Pluton could have involved large-scale consistently oriented translation and associated shearing, rather than passive "blister-like” coaxial deformation associated with pluton ballooning. It should be noted that mylonitic deformation is restricted to the western half of the pluton, features indicative of a more "permitted” emplacement mechanism being found in the eastern portion of the pluton. The detected top-to-the-SE shear-sense could be interpreted as indicating that the granitic material forming the western part of the pluton was forcibly intruded in a northwestward direction from the pluton source as a nearly solidified wedge beneath a static cover of sedimentary rocks. Alternatively, the detected shear sense could also be interpreted as indicating SE-directed thrusting of the cover rocks over the underlying pluton, the western margin of the pluton suffering intense mylonitic deformation, while the eastern margin was located in a "stress-shadow” region. If this alternative interpretation is correct, then the deformation temperatures indicated by the pattern of quartz c-axis fabrics dictate that thrusting must either be synchronous with pluton emplacement, or at least have commenced during the early stages of pluton coolin

Influence of gas flow rate on liquid distribution in trickle-beds using perforated plates as liquid distributors

Two wire mesh tomography devices and a liquid collector were used to study the influence of the gas flow rate on liquid distribution when fluids distribution on top of the reactor is ensured by a perforated plate. In opposition to most of the studies realized by other authors, conditions in which the gas has a negative impact in liquid distribution were evidenced. Indeed, the obtained results show that the influence of gas flow rate depends on the quality of the initial distribution, as the gas forces the liquid to "respect" the distribution imposed at the top of the reactor. Finally, a comparison between the two measuring techniques shows the limitations of the liquid collector and the improper conclusions to which its use could lead

A Keplerian Disk around the Herbig Ae star HD169142

We present Submillimeter Array observations of the Herbig Ae star HD169142 in 1.3 millimeter continuum emission and 12CO J=2-1 line emission at 1.5 arcsecond resolution that reveal a circumstellar disk. The continuum emission is centered on the star position and resolved, and provides a mass estimate of about 0.02 solar masses for the disk. The CO images show patterns in position and velocity that are well matched by a disk in Keplerian rotation with low inclination to the line-of-sight. We use radiative transfer calculations based on a flared, passive disk model to constrain the disk parameters by comparison to the spectral line emission. The derived disk radius is 235 AU, and the inclination is 13 degrees. The model also necessitates modest depletion of the CO molecules, similar to that found in Keplerian disks around T Tauri stars.Comment: 10 pages, 2 figures, accepted by A

A double-lined spectroscopic orbit for the young star HD 34700

We report high-resolution spectroscopic observations of the young star HD 34700, which confirm it to be a double-lined spectroscopic binary. We derive an accurate orbital solution with a period of 23.4877 +/- 0.0013 days and an eccentricity of e = 0.2501 +/- 0.0068. The stars are found to be of similar mass (M2/M1 = 0.987 +/- 0.014) and luminosity. We derive also the effective temperatures (5900 K and 5800 K) and projected rotational velocities (28 km/s and 22 km/s) of the components. These values of v sin i are much higher than expected for main-sequence stars of similar spectral type (G0), and are not due to tidal synchronization. We discuss also the indicators of youth available for the object. Although there is considerable evidence that the system is young --strong infrared excess, X-ray emission, Li I 6708 absorption (0.17 Angstroms equivalent width), H alpha emission (0.6 Angstroms), rapid rotation-- the precise age cannot yet be established because the distance is unknown.Comment: 17 pages, including 2 figures and 2 tables. Accepted for publication in AJ, to appear in February 200