Antibiotic susceptibility of Staphylococcus aureus and Escherichia coli isolated from dairy goats in selected farms in Selangor, Malaysia

Antimicrobial resistance (AMR) has become a major problem worldwide with significance public health impact in both animal and human populations. Staphylococcus aureus (S. aureus) is a common pathogen in domestic livestock and Escherichia coli (E. coli) is a normal flora in the gut and these organisms are considered as effective indicators for AMR surveillance. Increased in antimicrobial resistance of these bacteria in veterinary medicine leads to difficulties in choosing effective antibiotics to treat diseases they caused. This study aimed to evaluate the susceptibility of S. aureus and E. coli isolated from dairy goats in selected farms located in Selangor, Malaysia against different antibiotics. Milk and faecal samples were collected from 36 dairy goats and samples were prepared for the isolation of S. aureus and E. coli isolates respectively. The isolates were subjected to antibiotic susceptibility test. All 11 (100%) E. coli isolates showed resistance to amoxicillin and penicillin while 3 (27%) of the 11 S. aureus isolates, showed resistance towards the same class of antibiotic. Both S. aureus and E. coli isolates showed high susceptibility to four antibiotics, namely trimethoprim-sulfamethazole, neomycin, tetracycline and enrofloxacin. This study provided information regarding the antibiotic resistance of S. aureus and E. coli in relation to the antimicrobial usage practice in selected dairy goat farms located in Selangor, Malaysia

The Impact of Lameness on Dairy Cattle Welfare: Growing Need for Objective Methods of Detecting Lame Cows and Assessment of Associated Pain

Dairy cows are the major animals reared for milk production worldwide. Lameness is a manifestation of painful condition due to injury or disease in the foot, regarded as a major welfare problem in dairy cows. An effective lameness management requires prompt identification of lame cows. The objectives of this systematic review were to discuss the various techniques of detecting lameness, assessment of the associated pain, and the impact of lameness on dairy cow welfare. Results from the literature search yielded 534 papers, with 102 papers meeting the inclusion criteria. The eligible studies were discussed in two sections which were; (1) lameness detection systems and their application in pain assessment using four methods: gait and behavioral variables, physiological parameters, pressure nociceptive threshold and blood biomarkers; (2) impact of lameness on animal-based welfare measures. Despite the limitations in the use of automated locomotion scoring systems, results showed the technique remains a promising tool for the prompt detection of lame cows compared with manual systems (MLSS). More investigation of such systems could aid the validation of pain in cows with various degree of lameness. Further studies are required for early lameness detection and minimizing the welfare implications in dairy herds

Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model

The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblast cells were suspended in autologous fibrin polymerized separately to form a tissue-engineered respiratory epithelial construct (TEREC). A 3 × 2 cm2 tracheal mucosal defect was created, and implanted with TEREC and titanium mesh as a temporary scaffold. The control groups were divided into 2 groups: polymerized autologous fibrin devoid of cells (group 1), and no construct implanted (group 2). All sheep were euthanized at 4 weeks of implantation. Gross observation of the trachea showed minimal luminal stenosis formation in the experimental group compared to the control groups. Macroscopic evaluation revealed significant mucosal fibrosis in control group 1 (71.8%) as compared to the experimental group (7%). Hematoxylin and eosin staining revealed the presence of minimal overgrowth of fibrous connective tissue covered by respiratory epithelium. A positive red fluorescence staining of PKH26 on engineered tissue 4 weeks after implantation confirmed the presence of cultured nasal respiratory epithelial cells intercalated with native tracheal epithelial cells. Scanning electron microscopy showed the presence of short microvilli representing immature cilia on the surface of the epithelium. Our study showed that TEREC was a good replacement for a tracheal mucosal defect and was able to promote natural regenesis of the tracheal epithelium with minimal fibrosis. This study highlighted a new technique in the treatment of tracheal stenosis

Dairy farmers perceptions of and actions in relation to lameness management

Lameness continues to be a welfare and economic issue for dairy cows. However, the consequences of lameness seem to be better understood by veterinarians and related personnel in comparison to dairy farmers. Prompt detection and treatment of lame cows is essential in reducing its negative impact on milk processing systems. To that end, understanding farmers’ perceptions regarding the significance of lameness to dairy cows is vital. One fundamental aspect is the underestimation of lameness prevalence by dairy farmers, which is as a result of different understanding of the problem. The same applies to their decision to treat lame cows and to adopt various detection and management practices. All of these shortcomings contribute to poor cattle welfare and economic losses in dairy production. This review summarizes the results of studies that have investigated dairy farmers’ perceptions of lameness and the associated implications on the wellbeing and productivity of dairy cows. Factors associated with farmers’ attitudes toward claw health and lameness management are also presented. Additionally, economic observations relating to lameness prevention, treatment and the adoption of lameness detection systems are also highlighted. To strengthen these points, interventional programmes requiring farmers’ participation are discussed as a promising approach in answering some of these challenges. A review of the literature indicates both the opportunities and barriers inherent in the tackling the lameness issue from the farmers’ perspectives. Such knowledge is crucial in identifying measures on how to motivate dairy farmers towards proper lameness management

Prevalence of Toxoplasma gondii in pet and stray cats in Klang Valley, Malaysia

Toxoplasma gondii, a zoonotic protozoan that has a worldwide distribution, is known to infect many warm-blooded vertebrates. The feline species including domestic cats are the definitive hosts for Toxoplama gondii and shed the infective oocyst. There is lack of information on the prevalence of Toxoplasma gondii in cats in Malaysia. The objective of this study was to determine both the seroprevalence of T. gondii and the prevalence of T. gondii DNA in cats’ feces in Klang Valley, Malaysia. 198 blood and 201 fecal samples were collected from pet and stray cats from the local council, Dewan Bandaraya Kuala Lumpur (DBKL) and University Veterinary Hospital, Universiti Putra Malaysia respectively. The overall seroprevalence of Toxoplasma gondii in cats in the Klang Valley was found to be 5.5%. There was a high prevalence (10.5%) of T. gondii DNA detected in the cat fecal samples in both pet and stray cats suggestive of T. gondii oocyst shedding. Stray cats showed a higher seroprevalence and molecular prevalence of T. gondii than the pet cats. However, comparative analysis using Chi-square test showed no significant difference between both groups (P>0.05). Higher prevalence (10.5%) of cats shedding T. gondii DNA as compared to the seroprevalence (5.5%) was found in the cat population in the Klang Valley. The high prevalence of cats shedding T. gondii DNA is alarming as this may directly reflect the number of oocysts excreted into the environment posing a significant public health hazard

A longitudinal study of lameness incidence and association with animal-based welfare measures in dairy cows

The objective of this study was to investigate time to lameness event, prevalence of claw lesions, and their associations with animal-based welfare measures (ABWMs) in dairy cows. A total of 120 non-lame lactating cows (n = 30 each from four intensive dairy farms) having no claw lesion were enrolled in the study. The cow-level enrollment criteria included good body condition score (BCS), normal hock condition score, absence of body injuries, and normal claw length. Information on cows’ parity, milk yield, and previous lameness event (PLE) were recorded. The cows were observed twice monthly (every 2 weeks) for locomotion scores (LS), and ABWMs, whereas claw lesions were recorded upon onset of lameness and at the end of the observation period. Cows were considered lame when 2 consecutive LS = 3, or any assessment with score 4. Cox regression models were used to investigate lameness incidence and the association with ABWMs, while association between the latter and claw horn lesions (CHL) prevalence were analyzed using logistic regression models. Twenty-four percent (29/120) of the cows were lame during the study period. Lameness risk was associated with PLE (Hazard ratio; HR = 7.4; 95% CI 2.4-23.0), presence of overgrown claw (HR = 3.7; 95% CI 1.1-12.6) and low BCS pre-lame (HR = 4.5; 95% CI 1.3-16.6). Amongst the cows affected with claw lesions, 68.4% (37/44) were lame and CHL were predominant (75.4%) compared to infectious claw lesions (24.7%). Cows with lower BCS (< 3.0) (OR = 5.7; 95% CI 1.6- 20.4) and those with PLE (OR = 7.2; 95% CI = 2.1-24.7) were more likely to have CHL. Management practices such as maintaining cows’ body condition, improved care for those with history of lameness, and proper claw trimming could assist farmers to reduce lameness incidence in the studied herds

Cow- and herd-level factors associated with lameness in dairy farms in Peninsular Malaysia

Lameness is a major welfare issue in dairy cows. This study was aimed at investigating the cow- and herd –level factors associated with lameness in dairy farms from four states in Peninsular Malaysia. The study population was 1001 lactating cows from 28 dairy farms located in Selangor (n = 9), Perak (n = 8), Negeri Sembilan (n = 6) and Johor (n = 5). Lameness was assessed by locomotion scoring. Individual cow characteristics such as breeds, parity, body condition score (BCS), hock condition, leg hygiene, presence of claw lesion and claw overgrowth were recorded. Data on herd characteristics, management practices and housing design were collected by on-farm inspection and farmers’ interview. Mixed-eff ;ects logistic regressions were used to model the data and to assess the factors associated with lameness. Cow-level lameness prevalence was 34.2 % (95 % CI 22.2–50.0 %), with all the farms having at least one case of lameness. Claw lesions were recorded in 470 cows (46.9 %; CI 33.3–63.3 %) of which 296 (62.9 %) of them were lame. Of these, 78.9 % of the lesions were present on the rear feet and 25.5% of the cows had more than one foot affected. The proportion of cows having non-infectious and infectious claw lesions were 81.9 % and 18.1 %, and the predominant claw lesions were sole ulcers (24.9 %), white line disease (19.6 %), sole haemorrhage (10.2%), swelling of coronet (9.6 %), toe ulcers (8.4 %), and digital dermatitis (5.6%). Cows at third or more parities had higher odds of lameness (OR = 2.2; 95 % CI 1.2–4.1) compared to primiparous cows. Low BCS (< 2.5) increased the odds of lameness (OR = 4.8; 95 % CI 2.9–7.9) relative to cows with moderate BCS, and cows with hair loss around the hock (OR = 1.4; 95 % CI 1.1–1.9) relative to those with normal hock condition. Greater odds of being lame was observed in cows having claw lesion (OR = 15.2; 95 % CI 10.4–19.2) and those with overgrown claw (OR = 3.3; 95 % CI 2.4–4.5). There was increased odds of lameness in farms with high stocking density (OR = 1.8; 95 % CI 1.1−3.1), concrete floored walkways (OR = 1.9; 95 % CI 1.0–3.6), dirty floors (OR = 2.3; 95 % CI 1.9–3.7), and practicing preventive claw trimming (OR = 2.3; 95 % CI 1.9–4.6). Based on the high lameness prevalence, these findings could assist dairy farmers to make informed decisions on areas to implement on-farm changes to reduce lameness in the studied herds

Anterior cruciate ligament resection and medial meniscectomy result in multifocal cartilage degenerations

Damage to the meniscus and the anterior cruciate ligament alters the pattern of loading in the knee joint and frequently leads to cartilage degeneration and osteoarthritis. This study aimed to evaluate the area of greatest impact after traumatic osteoarthritic induction at the knee joint in sheep model. Osteoarthritis was induced unilaterally at the right hind knee of six sheep by surgical resection of the anterior cruciate ligament and medial meniscus. After 3 weeks recovery period, sheep were exercised once daily by running a 100 meters distance on a hard surface for 3 weeks. Gross and histological assessments were done. Six regions examined were; the patella, patella femoral groove, medial femoral condyle, lateral femoral condyle, medial tibia plateau and lateral tibia plateau. Their mean International Cartilage Repair Society grading was: 2.5±0.42, 3.3±0.17, 2.25±0.38, 1.33±0.25, 2±0.37 and 1.5±0.22 respectively. The control from all samples scored zero at the above regions. The lesions at various regions were significantly higher compared to the control (p < 0.05). Patella femoral groove was significantly higher compare to all other regions except for Patella. Lateral femoral condyle was the least (p < 0.05). Histological staining revealed defined osteoarthritic changes. Total excision of medial meniscus and anterior cruciate ligament followed by exercise regime induced osteoarthritis in the affected knee joint. The changes in pattern of degeneration, affected patella femoral groove the most; followed by the patella and medial femoral condyle, then medial tibia plateau, lateral tibia plateau and lastly the lateral femoral condyle

Handling practices and food safety measures about umai delights among Bintulu locals, Borneo Sarawak

Umai is a popular traditional dish among the Melanau ethnic group indigenous to Sarawak. The local people believed that the acidity of the citrus juice can slightly cook the fish and remove the fishy smell. The aim of the study was to investigate the umai preparation process and to study the food safety awareness among the locals in Bintulu. A purposive sample of 100 umai makers divided into two equal groups; the professionals and non-professionals, participated in the study. The most recommended fish for making umai in the professional and non-professional group were Spanish Mackerel (62%; 32/50) and Empirang (82%; 41/50), respectively. Majority of the respondents in both groups (>60%) determined the freshness of the fish by observing the condition of the gills. Both groups were using calamansi juice as their main ingredients, as well as vinegar, lemon juice, asam paya and salt. Only 12% of the professional and 14% of the non-professional respondents agreed on freezing the raw fish chunks before processing them into umai. Most of the respondents indicated, that soon after marinating the raw fish in calamansi juice with other ingredients, the umai dish is ready to eat. A higher proportion of the non-professional (60%) compared to the professional respondents (50%) affirmed umai need to be chilled for 30 min before serving. Overall, the respondents showed little understanding of food safety measures. Hence, this study revealed that, the handling and processing of umai, and the food safety measures need to be improved among the Bintulu population

Buffalo in Borneo, Sarawak: A Review of the Current Status of the Indigenous Buffalo Industry

The Malaysian water buffalo, Bubalus bubalis, has traditionally been important livestock for the local people of Sarawak. Buffalo, other ruminants, and non-ruminants are essential for the livestock industry as they supply the largest source of protein for the entire nation. Cattle provide the largest production in the livestock industry at 0.2%, followed by other ruminants, including goats (0.1%), sheep (0.04%) and buffalo (0.03%). Water buffalo, or 'kerbau sawah' as it is locally known, is an overlooked animal compared to other livestock. Amidst the growing demand for beef within the domestic consumer market, the buffalo population in Sarawak has risen over the past 5 years from 5,396 to 6,205 heads in 2019. However, the self-sufficiency level (SSL) of cattle and buffalo beef has dropped from 26% in 2013 to 21.6% in 2019 despite the rising demand for local produce. Malaysia continues to import frozen buffalo beef from India and live bovine from other countries in order to meet domestic demands and support the growth of the local buffalo industry. Due to this, the Department of Veterinary Services (DVS) Sarawak introduced a buffalo yard program ('Natad Kerbau') to assist the state smallholder farmers in managing their buffaloes better. This paper focuses on describing the current status of buffalo production in Sarawak, its important functions (including provision for food security) and future prospects of B. bubalis in the livestock industry of Malaysia and Sarawak in particular