Differences between the effects of water Naftussya from fields of Truskavets’ and Pomyarky on the parameters of the EEG, HRV, immunity and metabolism

Background. In previous studies involving subjects of both sexes with dysfunction of the neuroendocrine-immune complex, we found that the weekly use of Naftussya as Truskavets’ and Pomyarky deposits generally has the same effect on its parameters.The purpose of this study is to identify the differences between the effects of water of these deposits. Material and research methods. Observations were conducted with the participation of 22 volunteers with a clinical diagnosis chronic pyelonephritis with dysfunction of the neuroendocrine-immune complex and metabolism. At first, they collected a daily urine, which determined the content of electrolytes and nitrogen metabolites, and then in the morning they started taking samples of capillary and venous blood for biochemical, hormonal and immune assays, as well as registering the HRV and EEG. After the initial survey, 10 volunteers spent 7 days drinking water Naftussya from the Pomyarky (NP) deposit, while the remaining 12 from Truskavets deposit (NT) (250 ml three times a day), after which the above tests repeated. Results. The use of NP does not affect the symmetrical theta-and delta-rhythms, as well as causes the right asymmetry of the alpha-rhythm, whereas NT causes the left-hand asymmetry of all three rhythms. Increased asymmetry of alpha-rhythm and amplitude of delta-rhythm under the influence of NP decreases to normal, whereas NT causes even more deviation of these parameters from the norm. The use of NP reduces both the normal spectral power (SP) of VLF band of HRV and the increased SP of LF band without affecting the normal SP of HF band. Instead, NT increases the SP of LF and HF bands without affecting the VLF band. The described differences in neurotropic effects are accompanied by a more pronounced increase in blood levels of active T-lymphocytes and T-killers and more pronounced stimulation of NP intensity of phagocytosis by neutrophils of Staph. aureus and E. coli, as well as the activity and completeness of phagocytosis the latter's. This leads to a somewhat more pronounced reduction of bacteriuria. Conclusion. The revealed differences, apparently, are due to differences in the composition of organic substances and autochthonous microflora

Comparative investigation of immediate effects on neuro-endocrine-immune complex of bioactive water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a. Communication 1. Generic effects

Sydoruk Nadiya O, Zukow Walery. Comparative investigation of immediate effects on neuro-endocrine-immune complex of bioactive water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a. Communication 1. Generic effects. Journal of Education, Health and Sport. 2016;6(8):85-101. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.59762http://ojs.ukw.edu.pl/index.php/johs/article/view/3734   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 02.07.2016. Revised 25.07.2016. Accepted: 28.07.2016.  COMPARATIVE INVESTIGATION OF IMMEDIATE EFFECTS ON NEURO-ENDOCRINE-IMMUNE COMPLEX OF BIOACTIVE WATER NAFTUSSYA FROM LAYERS TRUSKAVETS’, POMYARKY AND SKHIDNYTS’A.Communication 1. GENERIC EFFECTS Nadiya O Sydoruk1, Walery Zukow2 1JSC “Dnipro-Beskyd”, Truskavets’, Ukraine   [email protected] of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland  [email protected] Abstracts Background. Ukraine has a number of fields Bioactive Water Type “Naftussya” focused on the Ukrainian Carpaty: Truskavets’, Skhidnytsya, Shklo, Mrazhnytsya, Opaka, Mizun’, Selyatyn, Guta etc and Podillya: Sataniv, Husyatyn, Makiv etc. Until now conducted comparative studies of their effects only on clinical symptoms as well as excretion of urine and bile. The aim of this study – compare immediate effects of Bioactive Water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a on neuro-endocrine-immune complex. Methods. The object of observation were 15 volunteers-men without clinical diagnose but with moderate disfunction of neuroendocrine-immune complex (disadaptation). In basal conditions and after drinking Control (distillated, filtered, well) and Bioactive Waters recorded EEG (“NeuroCom Standard”) and HRV (“Cardiolab+VSR”). In blood counted up Leukocytogram, on the basis of which determined Popovych’s Adaptation Index and Strain Index. In serum of venous blood determined content of principal adaptation Hormones: Cortisol, Testosterone and Triiodothyronine (ELISA) as well as Na+ and K+ (flaming photometry) for evaluation of Mineralocorticoide activity. Immune status evaluated on a set of I and II levels recommended by the WHO. Results. Observed cohort characterized disadaptation documented high frequentlyties of Disharmonious General Adaptation Reactions (53,7%) as well as Distress (11,1%), decreased Popovych’s Adaptation Index, plasma level of Triiodothyronine, Vagal tone, Completeness of Phagocytose, blood level of “Active”, Theophilline resistance and CD4+ T-Lymphocytes, CD16+ NK-Lymphocytes as well as IgA while increased Popovych’s Strain Index, plasma level of Cortisol, Mineralocorticoide activity, Bayevskiy’s Stress and Activity Regulatory Systems Indexes, Sympathetic tone, blood level of CD8+ T-Lymphocytes as well as IgM. By method od discriminant analysis it is detected 38 variables specific to Basal level and after 1,5 h after drink Control Waters (CW) and Bioactive Waters Naftusssya (BAWN). Discriminant variables divided on four clusters. First cluster reflects caused by BAWN reversion of activating trend after CW for 17 variables (changes in Euklidian Units relative to Basal Level makes -0,14±0,03 and +0,45±0,06 respectively) while second cluster reflects reversion of inhibiting trend for 12 variables (changes relative to Basal Level makes +0,38±0,09 and -0,20±0,07 respectively). Information about these 29 variables condensed in major canonical root (62,6% discriminant properties). Means of its makes for Basal Level (n=54) +0,05±0,13 Mahalanobis’ Units, after CW (n=15) -3,10±0,31 while after BAWN in total (n=39) +1,12±0,20, separately for layers Truskavets’ (n=12) +1,28±0,28, Pomyarky (n=12) +1,13±0,28 and Skhidnyts’a (n=15) +0,99±0,29. Third cluster reflects attenuation of activating trend for 4 variables (changes relative to Basal Level makes +0,47±0,08 and +0,25±0,02 respectively) while fourth cluster reflects reduction of inhibiting trend for 5 variables (changes relative to Basal Level makes -0,31±0,04 and -0,10±0,03 respectively). Information about these 9 variables condensed in minor canonical root (37,4% discriminant properties). Means of its makes for Basal Level +1,03±0,14, for CW -0,94±0,20, for layers Truskavets’ -1,03±0,30, Pomyarky -1,28±0,28 and Skhidnyts’a -0,93±0,30. Conclusion. Bioactive Water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a causes approximately equal immediate effects on 29 parameters of neuro-endocrine-immune complex different from effects of Control (distillated, filtered, well) Waters. Keywords: Bioactive Water Naftussya, EEG, HRV, Cortisol, Testosterone, Triiodothyronine, Leukocytogram, Immunity

Modulating Neuro-Endocrine Effects Of Bioactive Water Naftussya From Layers Truskavets’ And Pomyarky At Humans With Dysfunction Of Neuro-Endocrine-Immune Complex

Background. Previously we have been carry out comparative investigation long-term effects of Bioactive Water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a on some metabolic and biophysical parameters at men with dysfunction of neuro-endocrine-immune complex. The aim of this study is the investigation long-term effects on neuro-endocrine regulation at similar patients. Materials and methods. The object of observation were 20 volunteers: ten women and ten men aged 33-76 years without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism. We evaluated the trait and reactive anxiety, recorded EEG, HRV and blood pressure, determined levels of Cortisol, Aldosterone, Testosterone, Triiodothyronine and Calcitonin. After examination volunteers within 7 days used bioactive water Naftussya (250 mL three times a day) from Truskavets’ or Pomyarky layer, then repeated the tests listed. Results. Weekly drinking of Bioactive Water Naftussya normalizes initially elevated levels of testosterone in women and reduces initially normal its level in men, in both sexes normalizes initially elevated levels of triiodothyronine and activity of the autonomic regulatory systems as the parasympathetic, so sympathetic, combined with a slight increase in the normal levels of aldosterone and trend to increase (under low) reactive anxiety as well as post occlusive reactivity of vascular tone. Among the 164 registered parameters quantitative EEG revealed noticeable changes only 8. This is, above all, normalizing increase initially reduced spectral power density (SPD) α-rhythm in loci T6 by 93%, P4 by 33%, F7 by 29%, increase by 10% in the normal range modal frequency of β-rhythm and further enhance SPD θ-rhythm in locus T5 by 42%. On the other hand, showed a reduction by 28% initially normal PSD of β-rhythm in locus in O2 and further reduction its by 20% in locus P4. Moreover, there was left-sided lateralization initially symmetric α-rhythm. No significant differences between the effects of Naftussya both deposits were found. Conclusion. Bioactive water Naftussya both Truskavets’ and Pomyarky layers causes favorable normalizing effects on abnormalities neuro-endocrine parameters, which is a manifestation of its adaptogenic properties

Modulating effects of bioactive water Naftussya from layers Truskavets' and Pomyarky on neuro-endocrine-immune complex and metabolism at rats exposed to acute stress

Sydoruk Nadiya O, Gozhenko Anatoliy I, Zukow Walery. Modulating effects of bioactive water Naftussya from layers Truskavets’ and Pomyarky on neuro-endocrine-immune complex and metabolism at rats exposed to acute stress. Journal of Education, Health and Sport. 2016;6(11):715-730. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.227087 http://ojs.ukw.edu.pl/index.php/johs/article/view/4125 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015). 755 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 02.11.2016. Revised 22.11.2016. Accepted: 30.11.2016. MODULATING EFFECTS OF BIOACTIVE WATER NAFTUSSYA FROM LAYERS TRUSKAVETS’ AND POMYARKY ON NEURO-ENDOCRINE-IMMUNE COMPLEX AND METABOLISM AT RATS EXPOSED TO ACUTE STRESS Nadiya O Sydoruk1,2, Anatoliy I Gozhenko1,Walery Zukow3 1Ukrainian Scientific Research Institute of Transport Medicine Ministry of Health, Odesa [email protected] 2JSC “Dnipro-Beskyd”, Truskavets’, Ukraine [email protected] 3Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland [email protected] Abstracts Background. Previously we have been carry out integrated quantitative estimation of neuroendocrine, immune and metabolic responses to acute stress at male and female rats. The aim of this study is to compare the modulating effects of Bioactive Water Naftussya (BAWN) from layers Truskavets’ and Pomyarky on neuro-endocrine-immune complex and metabolism at rats exposed to acute stress. Materials and methods. The experiment is at white rats Wistar line. Rats were divided into intact and 5 test groups treated for seven days with tap (control) water, distilled water as well as table water "Truskavets’ka" and BAWN from Truskavets’ and Pomyarky layers. A day after the end of course animals test groups subjected to water immersion restraint stress. A day after stress determined HRV, endocrine, immune and metabolic parameters as well as gastric mucosa injuries and comparing them with parameters of intact animals. Results. BAWN both Truskavets’ and Pomyarky layers minimizes or obviates abnormal 15 parameters caused by stress, but does not affect the poststressory deviation of 7 parameters. On the other hand, BAWN causes deviation of 15 other parameters which little or do not change under stress. Distilled water and water "Truskavets’ka" affect some parameters the opposite way, other less favorable, but some more favorably than BAWN. Conclusion. BAWN both Truskavets’ and Pomyarky layers limit pathogenic effects of stress, and initiate or enhance its compensatory and sanogenic effects. Keywords: acute stress, HRV, hormones, immunity, metabolism, rats, bioactive water Naftussya

Modulating effects of bioactive water Naftussya from layers Truskavets' and Pomyarky on some metabolic and biophysic parameters at humans with dysfunction of neuro-endocrine-immune complex

Gozhenko Anatoliy I, Sydoruk Nadiya O, Babelyuk Valeriy Ye, Dubkova Galyna I, Flyunt Viktor R, Hubyts’kyi Viktor Yo, Zukow Walery, Barylyak Liliya G, Popovych Igor L. Modulating effects of bioactive water Naftussya from layers Truskavets’ and Pomyarky on some metabolic and biophysic parameters at humans with dysfunction of neuro-endocrine-immune complex. Journal of Education, Health and Sport. 2016;6(12):826-842. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.238225 http://ojs.ukw.edu.pl/index.php/johs/article/view/4162 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 754 (09.12.2016). 754 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Author (s) 2016; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.12.2016. Revised 20.12.2016. Accepted: 25.12.2016. MODULATING EFFECTS OF BIOACTIVE WATER NAFTUSSYA FROM LAYERS TRUSKAVETS’ AND POMYARKY ON SOME METABOLIC AND BIOPHYSIC PARAMETERS AT HUMANS WITH DYSFUNCTION OF NEURO-ENDOCRINE-IMMUNE COMPLEX Anatoliy I Gozhenko1, Nadiya O Sydoruk1,2, Valeriy Ye Babelyuk3, Galyna I Dubkova3, Viktor R Flyunt2, Viktor Yo Hubyts’kyi3, Walery Zukow4, Liliya G Barylyak5, Igor L Popovych5 1Ukrainian Scientific Research Institute of Transport Medicine Ministry of Health, Odesa [email protected] 2JSC “Dnipro-Beskyd”, Truskavets’, Ukraine [email protected] [email protected] 3Clinical sanatorium „Моldova”, Truskavets’, Ukraine [email protected] 4Faculty of Physical Education, Health and Tourism, Kazimierz Wielki University, Bydgoszcz, Poland [email protected] 5Laboratory of Experimental Balneology, OO Bogomoletz Institute of Physiology NAS, Kyiv, Ukraine [email protected] Abstracts Background. Previously we have been carry out comparative investigation immediate effects of Bioactive Water Naftussya from layers Truskavets’, Pomyarky and Skhidnyts’a on neuro-endocrine-immune complex at men with its dysfunction. The aim of this study is the influence of the use of the course of Bioactive Water Naftussya from layers Truskavets’ and Pomyarky on some metabolic and biophysical parameters at similar patients. Materials and methods. The object of observation were 20 volunteers: ten women and ten men aged 33-76 years without clinical diagnose but with dysfunction of neuro-endocrine-immune complex and metabolism. In daily urine and venous blood we determined the content of electrolytes, nitrogenous metabolites and lipids, recorded conductivity of acupuncture points, rate of electronegative nuclei of buccal epithelium as well as parameters of gas discharge vizualisation (GDV). After examination volunteers within 7 days used bioactive water Naftussya (250 mL three times a day) from Truskavets’ or Pomyarky layer, then repeated the tests listed. Results. Weekly use of Bioactive Water Naftussya increases in the normal level of plasma chloride and sodium, normalizes low level of bicarbonate and decreases within the normal levels of potassium and phosphate. Urinary excretion of sodium and chloride increases while excretion and concentration of uric acid decreases, as the urine concentration of phosphates. The index lithogenicity urine decreased from 112% to 103% norm standard. Initially reduced level of plasma triacylglycerides increases, while decreases in the normal level of cholesterol in low-density lipoprotein composition. Among the biophysical parameters detected increase in the normal conductivity acupuncture points Pg (ND) at right side, which represent the nervous system, and left shift the ratio between the conductivity of acupuncture points MC (AVL), which represents the immune system. Increases electrokinetic index of epithelial cells of cheek to 1,7±0,3%, that indicating the "rejuvenation" of the organism to 1,4±0,3 years. We first discovered changes in parameters of GDV, namely elimination of asymmetries of virtual chakras (first, second and third) as well as reducing the excess energy of the first chakra. No significant differences between the effects of Bioactive Water Naftussya both fields generally not found. Conclusion. Bioactive water Naftussya both Truskavets’ and Pomyarky layers causes favorable normalizing effects on abnormalities parameters of metabolism and gas discharge visualization, which is a manifestation of its adaptogenic properties. Keywords: bioactive water Naftussya, electrolytes, nitrogenous metabolites, lipides, electroskin conductivity, gas discharge vizualisation